We narrowed to 3,214 results for: ER;

1. pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

   Plasmid

   #185678

   Purpose

   Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERmembrane-HaloTag-KKMP
   

   Expression

   Mammalian

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. EcMBP165-cpGFP.PPYF.T203V.pRSET

   Plasmid

   #33373

   Purpose

   a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

   Depositor

   Loren Looger

   Article

   Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

   Insert

   Improved MBP based Maltose Biosensor
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   cpGFP146 variant from GCaMP2 inserted between AA1…

   Promoter

   T7

   Available Since

   Dec. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-INU-FLAG-FAM134Bs

   Plasmid

   #128261

   Purpose

   Expresses FAM134Bs in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

   Insert

   FAM134Bs (RETREG1 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFSynW Syt1 WT IRES mRuby3

   Plasmid

   #170639

   Purpose

   Expression of syt1 in cultured neurons by lentiviral transduction

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Use

   Lentiviral

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFSynW Syt1 D303G IRES mRuby3

   Plasmid

   #170640

   Purpose

   Expression of syt1 in cultured neurons by lentiviral transduction

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Use

   Lentiviral

   Mutation

   D303G

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFSynW Syt1 D365E IRES mRuby3

   Plasmid

   #170641

   Purpose

   Expression of syt1 in cultured neurons by lentiviral transduction

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Use

   Lentiviral

   Mutation

   D365E

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX Syt1 C2AB (96-421) D303G

   Plasmid

   #170646

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Mutation

   D303G

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX Syt1 C2AB (96-421) D365E

   Plasmid

   #170649

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Mutation

   D365E

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX Syt1 C2AB (96-421) I367T

   Plasmid

   #170652

   Purpose

   Expression of syt1 for purification in E. Coli

   Depositor

   Edwin Chapman

   Article

   Bradberry et al Neuron. 2020 Jul 8;107(1):52-64.e7. doi:

   Insert

   SYT1 (Syt1 Rat)
   

   Expression

   Bacterial

   Mutation

   I367T

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Rtn2b-mNeon

    Plasmid

    #186598

    Purpose

    Encodes human full-length Reticulon 2 isoform B fused to mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Human Reticulon 2 isoform B (RTN2 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. EGFP-Miro1v4

    Plasmid

    #174111

    Purpose

    Expresses the peroxisomal variant (splice variant 4) of Miro with an N-terminal EGFP.

    Depositor

    Pietro De Camilli

    Article

    Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    Miro1 (var4, peroxisomal splice variant)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEM705 Gso (Cys)

    Plasmid

    #109375

    Purpose

    GsX chimera with Gαo C-terminus (Pertussis toxin sensitive)

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    Gso chimera
    

    Tags

    None

    Expression

    Mammalian

    Mutation

    Cys at position 391 (Pertussis Toxin Sensitive)

    Promoter

    CAG

    Available Since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. Cenp-B INCENP GFP

    Plasmid

    #45238

    Purpose

    Target INCENP to centromeres via full length CENP-B

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    Cenp-B, INCENP-Δcen, GFP
    

    Tags

    GFP and full length CENP-B

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-cGAS g3 Cas9-T2A-mCherry

    Plasmid

    #164254

    Purpose

    Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgcGAS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMT-Diaphanous-GFP

    Plasmid

    #24287

    Depositor

    Ron Vale

    Article

    Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

    Insert

    Diaphanous (dia Fly)
    

    Tags

    GFP

    Expression

    Insect

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pRN3P_TIR1

    Plasmid

    #119766

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    TIR1
    

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-p53 gRNA-Cas9-T2A-mCherry

    Plasmid

    #164256

    Purpose

    Transcription of p53 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sg p53
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. LacI INCENP

    Plasmid

    #45236

    Purpose

    Target INCENP to lac operator sequence

    Depositor

    Michael Lampson

    Article

    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

    Insert

    LacI, INCENP-Δcen, mCherry (lacI )
    

    Tags

    LacI (lac repressor protein) and mCherry

    Expression

    Mammalian

    Mutation

    CEN box deleted from INCENP (aa1-46)

    Promoter

    t7

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT2-Tbait-UAS-Positron-ST

    Plasmid

    #129266

    Purpose

    UAS-driven expression of Positron in zebrafish

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

    Insert

    Positron-ST
    

    Use

    Zebrafish expression

    Mutation

    D92N, E199V

    Available Since

    Aug. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDX_init containing Sb-loop

    Plasmid

    #132697

    Purpose

    pDX_init plasmid containing a loop Sybody for the generation of a qPCR standard curve

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    Sb_loop
    

    Use

    M13 phagmid vector

    Promoter

    lac

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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