We narrowed to 1,090 results for: FIB

1. pRCA377 - pBA904 Puro-T2A-GFP CD55 CRISPRa guide 1 (pRCA360 backbone)

   Plasmid

   #238168

   Purpose

   Lentiviral CRISPR guide vector expressing CD55 targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

   Depositor

   Thomas Norman

   Article

   Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

   Insert

   CD55 sgRNA CRISPRa (CD55 Human, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRCA810 - pBA904 Puro-T2A-GFP HES7 g2 CRISPRa guide (pRCA360 backbone) 674

   Plasmid

   #238177

   Purpose

   Lentiviral CRISPR guide vector expressing a HES7 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

   Depositor

   Thomas Norman

   Article

   Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

   Insert

   HES7 sgRNA CRISPRa (HES7 Human, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRE

   Plasmid

   #229544

   Purpose

   Lentiviral construct to express synthetic miRNA and OCT4-mNeonGreen fusion protein

   Depositor

   Domitilla Del Vecchio

   Article

   Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

   Insert

   Oct4(co)-mNeonGreen (POU5F1 Human)
   

   Use

   Lentiviral

   Tags

   Oct4-mNeonGreen

   Expression

   Mammalian

   Mutation

   codon-optimized OCT4

   Promoter

   TRE12

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. ATX3-D1

   Plasmid

   #184246

   Purpose

   Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)

   Depositor

   Sandra Macedo Ribeiro

   Article

   Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

   Insert

   Ataxin-3 Josephin domain and UIM1 (ATXN3 Human)
   

   Tags

   6x His-Tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Premature stop codon N263X

   Promoter

   T7 promoter

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pnCS_SEPT7_SEPT9_i1-i5-TEV-Strep

   Plasmid

   #180314

   Purpose

   bacterial co-expression of human SEPT7 and of human SEPT9_i1-i5

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Inserts

   SEPTIN7 (SEPTIN7 Human)
   SEPTIN9 (SEPTIN9 )
   

   Tags

   TEV-Strep

   Expression

   Bacterial

   Mutation

   SEPT9_i1-i5

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_SEPT9_i1 S22A S23A-msfGFP

   Plasmid

   #180338

   Purpose

   mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 S22A S23A

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_SEPT9_i1 R10A R15A-msfGFP

   Plasmid

   #180336

   Purpose

   mammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 R10A R15A

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_SEPT9_i1 S12A S13A-msfGFP

   Plasmid

   #180337

   Purpose

   mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

   Insert

   SEPTIN9_v1 (SEPTIN9 Human)
   

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   SEPT9_i1 S12A S13A

   Promoter

   CMV

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP Kind2 S159/181/666E

   Plasmid

   #105314

   Purpose

   To study altered downstream functions of phospholylation-site mimetic kindlin2

   Depositor

   Kenneth Yamada

   Article

   Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

   Insert

   Kindlin2 (FERMT2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S159/181/666E

   Available Since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4-AARE-luc2P-Hygro

    Plasmid

    #101787

    Purpose

    Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    3xAARE
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Promoter

    minimal TATA-box promoter with low basal activity

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTight-9-124-BclxL

    Plasmid

    #60857

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses a synthetic cluster of miR-9/9* and miR-124 fused to Bcl-xL under a doxycycline-inducible promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    miR-9/9*-miR-124-BCLxL (BCL2L1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    pTight

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-28a(+)-FGF2-G3

    Plasmid

    #135521

    Purpose

    Engineered form of fibroblast growth factor 2 with improved thermostability

    Depositor

    Paul Burridge

    Article

    Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

    Insert

    fibroblast growth factor 2 (FGF2 Human)
    

    Tags

    6x His tag and Agg Thrombin Tag

    Expression

    Bacterial

    Mutation

    Codon optimized for E. Coli production

    Promoter

    T7 Promoter

    Available Since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid

    #99278

    Purpose

    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance

    Depositor

    Jordan Miller

    Article

    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

    Inserts

    H2B-mVenus-IRES-mCherry
    Puro
    

    Use

    Lentiviral

    Tags

    H2B, IRES, PGK, mCherry, and mVenus

    Promoter

    EF1 alpha-human and PGK-mouse

    Available Since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSAM2_mCherry_Gata6

    Plasmid

    #72694

    Purpose

    Doxycyline regulated expression of Gata6 in mammalian cells

    Depositor

    Timothy Kamp

    Article

    Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

    Insert

    Gata 6 (Gata6 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV-Doxycycline

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3. Flag-CSL WT

    Plasmid

    #167004

    Purpose

    Mammalian expression of CSL WT

    Depositor

    Gian-Paolo Dotto

    Article

    Bottoni et al Nat Commun. 2019 Aug 29;10(1):3884. doi:

    Insert

    CSL (RBPJ Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-CMV-Blast-DNTGFBR2-HA

    Plasmid

    #130888

    Purpose

    Expresses dominant negative mutant human TGFBR2

    Depositor

    Gian-Paolo Dotto

    Article

    Bordignon et al Cell Rep. 2019 Aug 27;28(9):2358-2372.e6

    Insert

    TGFBR2 (TGFBR2 Human)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Dominant Negative receptor lacking cytosolic cata…

    Promoter

    CMV

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCX-HO1-2A-EGFP

    Plasmid

    #74672

    Purpose

    Mammalian expression of HO1 and EGFP

    Depositor

    Roberto Giovannoni

    Article

    Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

    Insert

    HO1 (HMOX1 Human)
    

    Tags

    2A-EGFP

    Expression

    Mammalian

    Promoter

    pCAGGS

    Available Since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-IDO1-p2a-eGFP

    Plasmid

    #187026

    Purpose

    Expresses IDO1 and GFP (seperately) in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Maassen et al Free Radic Biol Med. 2022 Aug 1;188:287-

    Insert

    Indoleamine 2,3-dioxygenase 1 (IDO1 Human)
    

    Tags

    P2A-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. ATX3-13Q

    Plasmid

    #184248

    Purpose

    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)

    Depositor

    Sandra Macedo Ribeiro

    Article

    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

    Insert

    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Human, Synthetic)
    

    Tags

    6x His-Tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    C-terminal codon optimization for protein expres…

    Promoter

    T7 promoter

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-RNF2-ngRNA+41_EF1a-puroR

    Plasmid

    #207358

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    RNF2 +41 ngRNA
    

    Use

    Lentiviral

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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