We narrowed to 7,725 results for: NAP

1. pcDNA5-FRT-TetO-fSNAPc

   Plasmid

   #106986

   Purpose

   open reading frame of fSNAP inserted into pcDNA5-FRT-TetO using the HindIII and XhoI restriction sites

   Depositor

   Melissa Moore

   Article

   Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

   Insert

   fSNAP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-Synapsin-mTurquoise2-C1-WPRE

   Plasmid

   #159953

   Purpose

   untargeted marker

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   mTurquoise2-C1
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1

   Plasmid

   #98976

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 1 and Cas9(N863A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A

   Plasmid

   #106185

   Purpose

   Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Has Service

   AAV1

   Insert

   SF-Venus-iGluSnFR.S72A
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: S72A

   Promoter

   hSynapsin

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-PGK-SNAP-MD-SOX2

   Plasmid

   #115687

   Purpose

   Constitutive expression of a SOX2 protein tagged with SNAP-tag and a mitotic degron (MD)

   Depositor

   David Suter

   Article

   Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

   Insert

   SNAP-MD-SOX2
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184V

   Plasmid

   #106184

   Purpose

   Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

   Insert

   SF-Venus-iGluSnFR.A184V
   

   Use

   AAV

   Tags

   Expression

   Mutation

   SFGFP: T203Y, F46L, T65G, S72A GltI: A184V

   Promoter

   hSynapsin

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.hSynap.ChETA(E123T/H134R)-eYFP.WPRE.hGH

   Plasmid

   #100049

   Purpose

   AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by hSynap promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Has Service

   AAV9

   Insert

   hChR2(E123T/H134R)
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   humanized, E123T/H134R

   Promoter

   hSynap

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCit-Snap25b D179K A199R

   Plasmid

   #53259

   Purpose

   SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   SNAP-25B (Snap25 Rat)
   

   Use

   Tags

   4aa-linker and mCitrine

   Expression

   Mammalian

   Mutation

   D179K and A199R

   Promoter

   CMV

   Available since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

9. Anti-Synaptotagmin-10 [N269/73R]

   Plasmid

   #114558

   Purpose

   Mammalian Expression Plasmid of anti-Synaptotagmin-10 (Mouse). Derived from hybridoma N269/73.

   Depositor

   James Trimmer

   Article

   Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

   Insert

   anti-Synaptotagmin-10 (Mus musculus) recombinant mouse monoclonal antibody (Syt10 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   dual CMV

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. ZM (pyrFEKO-HYG TetR-T7RNAP)

    Plasmid

    #31455

    Depositor

    George Cross

    Insert

    
    

    Use

    Floxed targeting vector for trypanosome

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pDEST17-His10-ccdB-SNAP

    Plasmid

    #166146

    Purpose

    Gateway destination vector for generation of N-terminal His10-tagged and C-terminal SNAP-tagged proteins in E. coli.

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Type

    Empty backbone

    Use

    Tags

    His10 and SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST17-His10-DDX5(kf)-SNAP

    Plasmid

    #166152

    Purpose

    Expression of N. furzeri DDX5 in E. coli

    Depositor

    Daniel Jarosz

    Article

    Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

    Insert

    DDX5
    

    Use

    Tags

    His10 and SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLV-PGK-SNAP-MD-OCT4

    Plasmid

    #115691

    Purpose

    Constitutive expression of an OCT4 (Pou5f1) protein tagged with SNAP-tag and a mitotic degron (MD)

    Depositor

    David Suter

    Article

    Friman et al eLife. 2019 Dec 3;8. pii: 50087. doi: 10

    Insert

    SNAP-MD-OCT4
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP humanAPOBEC1 L173A/G227A

    Plasmid

    #167171

    Purpose

    Expresses human APOBEC1 L173A/G227A in mammalian cells

    Depositor

    Silvestro Conticello

    Article

    Chieca et al bioRxiv 2021

    Insert

    humanAPOBEC1 (APOBEC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Leucine 173 to Alanine; changed Glycine 2…

    Promoter

    CMV promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJEP220-AAV-0.5Synapsin-GFP-pA

    Plasmid

    #62920

    Purpose

    AAV vector backbone designed to express GFP from a 0.5Synapsin promoter. It also contains a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    0.5synapsin

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJEP219-AAV-1.1Synapsin-GFP-pA

    Plasmid

    #62921

    Purpose

    AAV vector backbone designed to express GFP from a 1.1Synapsin promoter. It also contains a poly-Adenylation Signal (pA)

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    1.1Synapsin

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-Synapsin-mScarlet-C1-WPRE

    Plasmid

    #159955

    Purpose

    untargeted marker

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    mScarlet-C1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc-Cdc3-Cdc10-SNAP

    Plasmid

    #131161

    Purpose

    Expression of Septin rods

    Depositor

    Nils Johnsson

    Article

    Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

    Insert

    Cdc3 + Cdc10-SNAP
    

    Use

    Bicistronic

    Tags

    Cdc10: SNAP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGS-21a his6-GST-WTPhi29DNAP

    Plasmid

    #166863

    Purpose

    To produce a dual affinity-tagged Phi29 DNA polymerase

    Depositor

    Paul Blainey, Stephen Quake

    Article

    Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

    Insert

    Phi29 Polymerase codon-optimized for overexpression in E. coli
    

    Use

    Tags

    GST and his6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. #1.4 post-R-SynapShot-Lyn-miRFP670

    Plasmid

    #214718

    Purpose

    Expresses red #1.4 post-SynapShot and Lyn-miRFP670

    Depositor

    Won Do Heo

    Article

    Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

    Insert

    #1.4 post-R-SynapShot
    

    Use

    Tags

    Lyn-miRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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