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Showing: 801 - 820 of 2754 results

  9. pX030

    Plasmid
    #167154
    Purpose
    MoClo-compatible Level 1 (position 5) vector encoding Neonothopanus nambi caffeoylpiruvate hydrolase nnCPH under control of 0.4 kb 35S promoter, for expression in plants
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    p35s-nnCPH-ocsT
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    p35s
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167154-plasmid-map-sequence-id-335275

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  10. pX026

    Plasmid
    #167153
    Purpose
    MoClo-compatible Level 1 (position 4) vector encoding Neonothopanus nambi luciferase nnLuz with C-terminal His-tag under control of 0.4 kb 35S promoter, for expression in plants
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    p35s-nnLuz-ocsT
    Use
    Luciferase
    Tags
    His6-tag
    Expression
    Plant
    Mutation
    Promoter
    p35s
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167153-plasmid-map-sequence-id-335274

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  12. pX022

    Plasmid
    #167148
    Purpose
    MoClo-compatible Level 0-CDS2 promoterless vector encoding C-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    C-part of fungal hispidin synthase, nnHispS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167148-plasmid-map-sequence-id-335231

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  13. pX021

    Plasmid
    #167147
    Purpose
    MoClo-compatible Level 0-SP promoterless vector encoding N-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    N-part of fungal hispidin synthase, nnHispS
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167147-plasmid-map-sequence-id-335230

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  14. CIRTS-control

    Plasmid
    #213788
    Purpose
    CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:
    Insert
    CIRTS-Calm3-U6-control gRNA
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Syn1, U6
    Available since
    Feb. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    213788-plasmid-map-sequence-id-424349

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  16. CIRTS-Gas5

    Plasmid
    #213172
    Purpose
    CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and Gas5 guide RNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Timothy Bredy
    Article
    Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:
    Insert
    CIRTS-Calm3-U6-Gas5 gRNA
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Syn1, U6
    Available since
    Feb. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    213172-plasmid-map-sequence-id-424348

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  17. pmEos2-N1 GBP (GBP-mEos2)

    Plasmid
    #162876
    Purpose
    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy
    Depositor
    Frederic Meunier
    Article
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Insert
    GFP Binding Protein tagged with mEos2
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162876-plasmid-map-sequence-id-321989

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  18. pHybr_r2a>mG2b-srt-his

    Plasmid
    #124804
    Purpose
    HDR template to knock-in mouse IgG2b Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2b producing cell lines.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2b
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124804-plasmid-map-sequence-id-244314

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  19. pHybr_r2a>mG2a-srt-his

    Plasmid
    #124803
    Purpose
    HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Murine IgG2a
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124803-plasmid-map-sequence-id-244318

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  20. PX459-gR2A_Hinge

    Plasmid
    #124811
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Depositor
    Martijn Verdoes, Ferenc Scheeren
    Article
    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:
    Insert
    Cas9 – gRNA_R2A_hinge
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Bacterial and Mammalian
    Mutation
    R166H in PuroR (please see depositors comment bel…
    Promoter
    pUC
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    124811-plasmid-map-sequence-id-243700

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Showing: 801 - 820 of 2754 results