Addgene

We narrowed to 2,402 results for: gcg

Showing: 801 - 820 of 2402 results

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lenti-ERCC1 gRNA2

Plasmid

#211631

Purpose

sgRNA-2 against ERCC1

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ERCC1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDT4

Plasmid

#199120

Purpose

scrambled promoter circular DNA template

Depositor

Jeff Gelles

Article

Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

Insert

synthetic transcription template

Use

Tags

Expression

Bacterial

Mutation

mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …

Promoter

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE010-gR085

Plasmid

#188554

Purpose

Binary vector for CRISPR/Cas9 targeted to MpIGPD in Marchantia polymorpha (for Agrobacterium-mediated genetic transformation)

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

gR085

Use

CRISPR; Entry vector

Tags

Expression

Mutation

Promoter

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-gR085

Plasmid

#188552

Purpose

Gateway entry vector for sgRNA targeted to MpIGPD.Transient expression of sgRNA targeted to MpIGPD in Marchantia polymorpha.

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

gR085

Use

CRISPR; Entry vector

Tags

Expression

Mutation

Promoter

Available since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgDcc

Plasmid

#159906

Purpose

Mutagenesis of Dcc

Depositor

Larry Zweifel

Insert

Dcc (Dcc Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_THRA_iso1_2

Plasmid

#128246

Purpose

Encodes gRNA for 3' target of human THRA_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

THRA_iso1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_ZBTB49_iso1_1

Plasmid

#128250

Purpose

Encodes gRNA for 3' target of human ZBTB49_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB49_iso1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_ZBTB49_iso1_2

Plasmid

#128251

Purpose

Encodes gRNA for 3' target of human ZBTB49_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZBTB49_iso1 gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pSLHDR_R1

Plasmid

#124209

Purpose

gRNA/Cas9 expression for 2nd editing using pSLHDR01, 02

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pWT029g

Plasmid

#96860

Purpose

guanine-agRNA (GFP activation, 18 nt blocker with bulges)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

guanine-agRNA (GFP activation, 18 nt blocker with bulges)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pWT029h

Plasmid

#96861

Purpose

(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

(d)guanine-agRNA (GFP activation,18 nt blocker with bulges)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBbA5k-AcrB/AcrD/AcrFa, ABO_0964

Plasmid

#86202

Purpose

Expresses efflux pump in E. coli to provide resistance to biofuel (medium copy)

Depositor

Mary Dunlop

Article

Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

Insert

Efflux pump

Use

Tags

Expression

Bacterial

Mutation

Promoter

ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLG-Acc

Plasmid

#33148

Depositor

Michael Rosbash

Article

Legrain et al Cell. 1989 May 19;57(4):573-83.

Insert

RP51A-synthetic minimal intron (RPS17A Budding Yeast)

Use

Tags

LacZ

Expression

Yeast

Mutation

AccI site in intron was cut/filled/ligated to cha…

Promoter

Available since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pLKO.1-blast-cfRab8b_1

Plasmid

#26708

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Oct 3. ():.

Insert

dog Rab8b RNAi

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Dog Rab8b RNAi

Promoter

Available since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only
pJB049

Plasmid

#22643

Depositor

Lynn Hudson

Article

Berndt et al J Biol Chem. 1992 Jul 25. 267(21):14730-

Insert

human genomic PLP promoter region (POU3F2 Human)

Use

Cat reporter plasmid

Tags

Expression

Mutation

Site 5: CCACC to gagCg (-238 to -234bp)

Promoter

Available since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
OA-1067B

Plasmid

#200252

Purpose

pBac-U6-gRNA(doublesex)-3xp3-tdTomato

Depositor

Omar Akbari

Article

Li et al bioRxiv. 2023 Nov 15:2023.04.18.537404.

Insert

4 gRNAs targeting Doublesex

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-Frame +0

Plasmid

#66939

Purpose

CRISPaint frame selector +0

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA frame +0

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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gCH128 (non-targeting crRNA)

Plasmid

#217339

Purpose

Non-targeting crRNA

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

crNT-3

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-Frame +1

Plasmid

#66940

Purpose

CRISPaint frame selector +1

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA frame +1

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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LC3B sgRNA

Plasmid

#207556

Purpose

pX330 expressing Cas9 and a sgRNA targeting the LC3B locus

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

CACTGAATACCAGCGCCTAG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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