We narrowed to 1,288 results for: transfection

1. pLEX307-SARS-CoV-3CL

   Plasmid

   #160280

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-syn-FLEX-jYCaMP1

   Plasmid

   #135422

   Purpose

   Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Insert

   jYCaMP1
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSGFPnERT(#205)

   Plasmid

   #184065

   Purpose

   cyclofen-inducible GFP nuclear relocation via mammalian cell transfection or mRNA synthesis

   Depositor

   Sophie Vriz

   Article

   Sinha et al Chembiochem. 2010 Mar 22;11(5):653-63. d

   Insert

   eGFP-nls-ERT2
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   sCMV+SP6

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX307-HCoV-NL63-3CL-C144A

   Plasmid

   #160287

   Purpose

   Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease with C144A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   C144A

   Promoter

   EF1a

   Available Since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLEX307-SARS-CoV-3CL-C145A

   Plasmid

   #160281

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV 3CL protease with C145A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   C145A

   Promoter

   EF1a

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLEX307-Bat-CoV-HKU9-3CL-C144A

   Plasmid

   #160285

   Purpose

   Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease with C145A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   C144A

   Promoter

   EF1a

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLEX307-MERS-CoV-3CL-C148A

   Plasmid

   #160283

   Purpose

   Allows for constituitive mammalian expression of the MERS-CoV 3CL protease with C145A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   C148A

   Promoter

   EF1a

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLEX307-IBV-3CL-C143A

   Plasmid

   #160289

   Purpose

   Allows for constituitive mammalian expression of the IBV 3CL protease with C143A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   C143A

   Promoter

   EF1a

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLAP-BUBR1-D882N

   Plasmid

   #114062

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Jun 12;22(6):1321-9. doi:

   Insert

   BUBR1-D882N (BUB1B Human)
   

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Mutation

   C2823A and G2826A (siRNA-resistance), BUBR1-D882N

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLAP-BUBR1-K795R

    Plasmid

    #114060

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Jun 12;22(6):1321-9. doi:

    Insert

    BUBR1-K795R (BUB1B Human)
    

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    C2823A and G2826A (siRNA-resistance), BUBR1-K795R

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBOB-Gpr45-Del1&2-3xFlag

    Plasmid

    #245981

    Purpose

    Express 3xFlag C-terminal tagged mouse GPR45-Del1&2 mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

    Insert

    Gpr45 (Gpr45 Mouse)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Del1&2, deletion of R223-D237 and R247-D259

    Promoter

    CMV

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBOB-HA-Gnas

    Plasmid

    #245994

    Purpose

    Express HA N-terminal tagged mouse Galphas transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

    Depositor

    Zhao Zhang

    Article

    Xun et al Science. 2025 Jun 5;388(6751):eadp3989.

    Insert

    Gnas (Gnas Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV mTagBFP2-2A-CHMP2B-Q165X

    Plasmid

    #232001

    Purpose

    Bicistronic expression of CHMP2B Q165X mutant along with an mTagBFP2 transfection marker, connected via 2A sequences.

    Depositor

    Franz-Ulrich Hartl

    Article

    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

    Insert

    CHMP2B (CHMP2B Human)
    

    Expression

    Mammalian

    Mutation

    Q165X

    Available Since

    Aug. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-U6-sgRNA-EF1a-LibVec

    Plasmid

    #239591

    Purpose

    AAV vector for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+) EGFP-Rac1(G12C, K96H)

    Plasmid

    #224281

    Purpose

    Cell transfection and expression of Rac1 (G12C, K96H)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rac1 (RAC1 Human)
    

    Expression

    Mammalian

    Mutation

    G12C, K96H

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1(+) EGFP-Rap1A(G12C, L96F)

    Plasmid

    #224277

    Purpose

    Cell transfection and expression of Rap1A (G12C, L96F)

    Depositor

    Kevan Shokat

    Article

    Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

    Insert

    Rap1A (RAP1A Human)
    

    Expression

    Mammalian

    Mutation

    G12C, L96F

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCSmChnERT(#99)

    Plasmid

    #184063

    Purpose

    cyclofen-inducible mCherry nuclear relocation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Sinha et al Chembiochem. 2010 Mar 22;11(5):653-63. d

    Insert

    mCherry-nls-ERT2
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    sCMV+SP6

    Available Since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCSm5En2CER4(#457)

    Plasmid

    #184090

    Purpose

    cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Rampon et al Development. 2015 May 15;142(10):1840-9.

    Insert

    5myc-chkEn2(C>S)-ERT2
    

    Use

    Synthetic Biology

    Tags

    5xMyc

    Expression

    Mammalian

    Mutation

    chkEn2: C175S; ERT2: G400V, M543A, L544A, deleted…

    Promoter

    sCMV+SP6

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLAP-BUBR1-K795A

    Plasmid

    #114061

    Purpose

    transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

    Depositor

    Geert Kops

    Article

    Suijkerbuijk et al Dev Cell. 2012 Jun 12;22(6):1321-9. doi:

    Insert

    BUBR1-K795A (BUB1B Human)
    

    Tags

    YFP-TEV-S- Human LAP

    Expression

    Mammalian

    Mutation

    C2823A and G2826A (siRNA-resistance), BUBR1-K795A

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGfa2(ABDx3)-nLac

    Plasmid

    #53133

    Purpose

    expression of nuclear LacZ driven by Gfa2 promoter with 3 additional copies of the gfa28 ABD segment; has about 100 times higher activity than pGfa2-nLac in transfected cells

    Depositor

    Michael Brenner

    Article

    de Leeuw et al J Neurosci Res. 2006 Apr;83(5):744-53.

    Insert

    Gfa2 (GFAP Human)
    

    Use

    Mouse Targeting

    Tags

    LacZ, NLS, and mP1

    Expression

    Mammalian

    Mutation

    -2163 to +47, with 3 copies of 3 copies of the gf…

    Promoter

    human Gfa2

    Available Since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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