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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 17,433 results for: tes

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Showing: 8181 - 8200 of 17433 results

  1. pCAGGS-RaichuEV-Rac1(T17N)

    Plasmid
    #164902
    Purpose
    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain
    Depositor
    Takeshi Imai
    Publication
    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RaichuEV-Rac1 (RAC1 Human)
    Expression
    Mammalian
    Mutation
    T17N mutation in the Rac1 domain which make the s…
    Available since
    Sept. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    164902-plasmid-map-sequence-id-340899

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  2. pcDNA3-eGFP-NLS-RNF168 delta RING

    Plasmid
    #133983
    Purpose
    mammalian expression vector of eGFP tagged RNF168 where the N-terminus of RNF168 has been deleted (including RING domain)
    Depositor
    Daniel Durocher
    Publication
    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RNF168 (RNF168 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    deletion of RING; siRNA resistant sequence: GAGGA…
    Promoter
    CMV
    Available since
    Nov. 4, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    133983-plasmid-map-sequence-id-261913

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  3. mApple-proα2(I)-G610C

    Plasmid
    #119841
    Purpose
    Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and mApple between signal sequence and exon 6
    Depositor
    Sergey Leikin
    Publication
    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Type I procollagen α2 chain (Col1a2 Mouse)
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Col1a2 exons 2-5 replaced by fluorescent tag, Gly…
    Promoter
    CMV
    Available since
    Jan. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119841-plasmid-map-sequence-id-233868

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  4. BMR1_02g00320-bio-His

    Plasmid
    #108028
    Purpose
    Expresses enzymatically monobiotinylated full-length BMR1_02g00320 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    BMR1_02g00320
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    108028-plasmid-map-sequence-id-213147

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  5. pGL3-p107 1*2*

    Plasmid
    #32387
    Depositor
    Julien Sage
    Publication
    Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    p107 promoter (Rbl1 Mouse)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    1st and 2nd E2F binding sites mutated: GCG-->A…
    Available since
    Sept. 27, 2011
    Availability
    Academic Institutions and Nonprofits only

  7. pCAG-Bmpr2ΔKinase gRNA resistant

    Plasmid
    #163411
    Purpose
    Expresses BMPR-2ΔKinase resistant to the gRNAs(#163388-90) in mammalian cells
    Depositor
    Takeshi Imai
    Publication
    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Bmpr2 (Bmpr2 Mouse)
    Expression
    Mammalian
    Mutation
    The kinase domain (202-500 aa) is deleted. gRNA-t…
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    163411-plasmid-map-sequence-id-338987

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  8. pCI-TPI_WT-4MS2-xrRNA-4H

    Plasmid
    #108371
    Purpose
    Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites upstream of xrRNA and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Publication
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Wild type TPI reporter with 4MS2 binding sites upstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108371-plasmid-map-sequence-id-209436

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  9. pCI-TPI_WT-xrRNA-4MS2-4H

    Plasmid
    #108370
    Purpose
    Expresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites downstream of xrRNA and allows detection via northern blot (4H binding sites)
    Depositor
    Niels Gehring
    Publication
    Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Wild type TPI reporter with 4MS2 binding sites downstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    108370-plasmid-map-sequence-id-209434

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  10. YFP(1-158)-beta-2 in pcDNAI/Amp

    Plasmid
    #54469
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-2. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Publication
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP(1-158)/beta-2 (GNB2 Aequorea victoria, Human)
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    YFP (1-158) includes a substitution of Met for Gl…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    54469-plasmid-map-sequence-id-114883

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  15. pUC-ODN2

    Plasmid
    #208643
    Purpose
    Empty vector for long single strand DNA (up to 1.5k) preparation. Further remove BsaI and BsmBI site in pUC-ODN (Addgene #208642).
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    Long single strand dna production
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208643-plasmid-map-sequence-id-418356

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  17. nCas9-UGI control for Target-AID (pRZ1047)

    Plasmid
    #131299
    Purpose
    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    131299-plasmid-map-sequence-id-254648

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  18. pET28-H12TEV

    Plasmid
    #208635
    Purpose
    for E. coli expression of large protein, insert 6xhis + TEV site using NdeI and BamHI site in pET28a(+), note that it is 6xHis-Thrombin-6xHis-TEV, not continuous 12xHis. BamHI in-frame with tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    6xhis
    Expression
    Bacterial
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208635-plasmid-map-sequence-id-433640

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  20. pET28-5'TwStrep

    Plasmid
    #208620
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Tags
    Twin Strep
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208620-plasmid-map-sequence-id-415919

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Showing: 8181 - 8200 of 17433 results