We narrowed to 15,726 results for: NOL

1. pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

   Plasmid

   #164611

   Purpose

   Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

   Insert

   Endocannabinoid sensor GRAB_eCB2.0
   

   Expression

   Mammalian

   Mutation

   S383T

   Promoter

   CMV

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA

   Plasmid

   #205317

   Purpose

   All-in-one AAV vector expressing ISYmu1 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   humanized ISYmu1 TnpB
   

   Use

   AAV

   Tags

   3 x HA

   Expression

   Mammalian

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL1777 (pEffector)

   Plasmid

   #160731

   Purpose

   Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins and crRNA
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA Flag ERbeta

   Plasmid

   #35562

   Depositor

   Harish Srinivas

   Article

   Zhang et al Mol Endocrinol. 2010 Sep;24(9):1737-47.

   Insert

   Estrogen Receptor beta (ESR2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   March 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNCS-Antares

   Plasmid

   #74279

   Purpose

   Constitutive expression of Antares in bacteria

   Depositor

   Michael Lin

   Article

   Chu et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   Antares
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)

   Plasmid

   #139446

   Purpose

   Lentiviral vector genome to ectopically express Firefly luciferase

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag Firefly luciferase
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYFAC-CH2-ubi-M-pyrG

   Plasmid

   #221132

   Purpose

   Expression of heterologous genes under alcohol induction optimised with ubi-M-pyrG marker. Promoters PalcA-T1-PalcS/M-T2-PaldA expression cassette; unique restriction sites: PacI, NotI, AsiSI, AscI.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al Microb Biotechnol. 2024 Sep;17(9):e70010

   Type

   Empty backbone

   Use

   Synthetic Biology; Expression in aspergillus and …

   Promoter

   PalcA-T1-PalcS/M-T2-PaldA expression cassette (al…

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9-TadCBEd-ivt-template

   Plasmid

   #193843

   Purpose

   Template for in vitro transcription of TadCBEd (with SpCas9)

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadCBEd with SpCas9
   

   Use

   In vitro transcription template

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX601-AAV-CMV-NLS-ISDge10-NLS-3xHA-bGHpA;U6-BsaI-reRNA

   Plasmid

   #205318

   Purpose

   All-in-one AAV vector expressing ISDge10 TnpB and its reRNA

   Depositor

   Haoyi Wang

   Article

   Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

   Insert

   humanized ISDge10 TnpB
   

   Use

   AAV

   Tags

   3 x HA

   Expression

   Mammalian

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPRi_Mxi1_yl

    Plasmid

    #91248

    Purpose

    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. YE1-BE4max-NG

    Plasmid

    #138159

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A + …

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDestTol2pA2-9xGRE

    Plasmid

    #74736

    Purpose

    GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

    Depositor

    Luisa Dalla Valle

    Article

    Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

    Insert

    EGFP
    

    Use

    Zebrafish expression

    Promoter

    nine GC-responsive elements (9xGRE) from the rat …

    Available Since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1-Puro CAG FUCCI

    Plasmid

    #136934

    Purpose

    donor plasmid for constitutive FUCCI expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

    Insert

    Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)
    

    Use

    Synthetic Biology

    Tags

    Clover and mKO2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

    Plasmid

    #115684

    Purpose

    NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.

    Depositor

    David Suter

    Article

    Mandic et al Methods. 2017 May 1;120:65-75. doi: 10.1

    Insert

    Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro
    

    Use

    Lentiviral

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 IKKE K38A flag

    Plasmid

    #26202

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    I-kappa-B kinase epsilon K38A (IKBKE Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Catalytic Lysine changed to Alanine (K38A)

    Available Since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDB_182

    Plasmid

    #216103

    Purpose

    CRISPRi, EF1a-driven dCas9-KRAB (ZIM3) (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSC50

    Plasmid

    #104824

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Glyma.08g081600, Glyma.05g126600 (Hen1ab). Also expresses Cas9 from Gmubi promoter.

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Insert

    Glyma.08g081600, Glyma.05g126600
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. SpCas9-NG-SsAPOBEC3B

    Plasmid

    #198536

    Purpose

    Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-SsAPOBEC3B
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Promoter

    EFS

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CROPseq-iT7

    Plasmid

    #211699

    Purpose

    NIS-Seq-compatible lentiviral vector for expressing sgRNAs

    Depositor

    Jonathan Schmid-Burgk

    Article

    Fandrey et al Nat Biotechnol. 2024 Dec 19. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpCas9 TadDE

    Plasmid

    #193837

    Purpose

    Express TadDE (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-DualEditor-Cas9D10A-2xUGI
    

    Expression

    Mammalian

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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