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pET28 thermo.9

Plasmid

#191882

Purpose

Encodes a GFP design, thermo.9

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

thermo.9

Tags

His Tag

Expression

Bacterial

Mutation

T167V T203H E222Q

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 basic.3

Plasmid

#191881

Purpose

Encodes a GFP design, basic.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

basic.3

Tags

His Tag

Expression

Bacterial

Mutation

T65S V68A S72A Y145S L220V V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 mid.2

Plasmid

#191880

Purpose

Encodes a GFP design, mid.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.2

Tags

His Tag

Expression

Bacterial

Mutation

T108I Y145I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 bleach.6

Plasmid

#191879

Purpose

Encodes a GFP design, bleach.6

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.6

Tags

His Tag

Expression

Bacterial

Mutation

V61L S72C Y145M H181L V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 slow.3

Plasmid

#191877

Purpose

Encodes a GFP design, slow.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

slow.3

Tags

His Tag

Expression

Bacterial

Mutation

Q69L S72V Y145S T167V L220V V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 basic.2

Plasmid

#191911

Purpose

Encodes a GFP design, basic.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

basic.2

Tags

His Tag

Expression

Bacterial

Mutation

S72A V112I Y145V L220V V224I

Promoter

T7

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP3-C93A-C96A_rpoA-U

Plasmid

#190962

Purpose

Test the U-to-C editing activity of KP3:C93A/C96A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP3

Tags

His

Expression

Bacterial

Mutation

DYW-KP:C93A/C96A

Promoter

T7

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6-C93A-C96A_rpoA-C

Plasmid

#190970

Purpose

Test the C-to-U editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:C93A/C96A

Promoter

T7

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP2-C93A-C96A_rpoA-U

Plasmid

#190958

Purpose

Test the U-to-C editing activity of KP2:C93A/C96A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP2

Tags

His

Expression

Bacterial

Mutation

DYW-KP:C93A/C96A

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-PLS-KP6-C93A-C96A_rpoA-U

Plasmid

#190971

Purpose

Test the U-to-C editing activity of KP6:C93A/C96A on AtrpoA editing site, in E. coli

Depositor

Bernard Gutmann

Article

Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

Insert

PLS-KP6

Tags

His

Expression

Bacterial

Mutation

DYW-KP:C93A/C96A

Promoter

T7

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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TTR C10S E63C

Plasmid

#190009

Purpose

Expression of human TTR in E coli

Depositor

Peter Wright

Article

Sun et al J Biol Chem. 2022 Jun 18;298(8):102162.

Insert

TTR (TTR Human)

Expression

Bacterial

Mutation

C10S, E63C

Promoter

T7

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET303-ColE1-T-P110A

Plasmid

#180237

Purpose

Expresses T domain of Colicin E1 (residues 1-190) with proline to alanine mutation at residue 110

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

Colicin E1

Tags

6x His-tag

Expression

Bacterial

Mutation

Truncation of ColE1 containing T domain of ColE1 …

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-MBP-bhaB5

Plasmid

#167812

Purpose

Expresses His6- and MBP-tagged BhaB5 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

BhaB5

Tags

His6, MBP

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET-UTX(420-633)-H6

Plasmid

#183773

Purpose

Express pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.

Depositor

Peter Harte

Article

Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

Insert

UTX

Tags

6 His tag at the C-terminus

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pPOLY_new

Plasmid

#179276

Purpose

Expresses CuCBP, CcCDP and BaSP tricistronically in E. coli BL21(DE3)

Depositor

Bernd Nidetzky

Article

Schwaiger et al Microb Cell Fact. 2022 Apr 9;21(1):61. d

Inserts

cellodextrin phosphorylase
cellobiose phosphorylase
sucrose phosphorylase (C8077_RS00435 Bifidobacterium alodescentis)

Use

Synthetic Biology

Tags

6xHIS and Strep-Tag II

Expression

Bacterial

Promoter

T7 lacO

Available Since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFGC-T7RibJ-RRvT

Plasmid

#173226

Purpose

Encodes RRvT fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

RRvT

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJ414-PCY1:S495A

Plasmid

#98151

Purpose

Expresses PCY1:S495A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:S495A

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed serine 495 to alanine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:W603A

Plasmid

#98153

Purpose

Expresses PCY1:W603A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:W603A

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed tryptophan 603 to alanine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:H695Q

Plasmid

#98154

Purpose

Expresses PCY1:H695Q from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:H695Q

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed histidine 695 to glutamine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJ414-PCY1:H695A

Plasmid

#98155

Purpose

Expresses PCY1H695A from Saponaria vaccaria possesing a N-terminal His6-tag (TEV cleavable) in E. coli.

Depositor

James Naismith

Article

Ludewig et al ACS Chem Biol. 2018 Mar 16;13(3):801-811

Insert

Peptide cyclase 1:H695A

Tags

His6-TEV

Expression

Bacterial

Mutation

Changed histidine 695 to alanine

Promoter

T7

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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