We narrowed to 59,448 results for: cloning

1. KTK_046

   Plasmid

   #180544

   Purpose

   Entry vector containing RBS 250 a.u. designed strength

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   sRBS 250 a.u. designed strength
   

   Expression

   Bacterial

   Available Since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. KTK_042

   Plasmid

   #180530

   Purpose

   Entry vector containing J23100 anderson promoter

   Depositor

   Tom Ellis

   Article

   Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

   Insert

   J23100 anderson promoter
   

   Expression

   Bacterial

   Available Since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR221 CARD8 ZUC

   Plasmid

   #169964

   Purpose

   pDONR221 for Gateway shuttling. Contains the CARD8 FIIND and CARD (L162M-L537, no stop codon)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBlastR-MCS-LexA-VP16-MiniWhite

   Plasmid

   #165900

   Purpose

   Blasticidin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable Empty Enhancer Driver
   

   Use

   Synthetic Biology

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221 CARD8 FIIND L370G

   Plasmid

   #169970

   Purpose

   pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F370G)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Gateway cloning vector

   Mutation

   L370G

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. E1_pENTR_L1-L4_NI_G1333-DddtoxA-C

   Plasmid

   #171724

   Purpose

   ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

   Insert

   C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
   

   Use

   Multisite gateway entry vector

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. E1_pENTR_L1-L4_NN_G1333-DddtoxA-C

   Plasmid

   #171728

   Purpose

   ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

   Insert

   C- and N-termini of TALEN, half of a cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
   

   Use

   Multisite gateway entry vector

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. E3_pENTR_L3-L2_NG_G1333-DddtoxA-C

   Plasmid

   #171732

   Purpose

   ptpTALECD vector assembly, Entry clone 1 with attL2 and L3

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

   Insert

   C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
   

   Use

   Multisite gateway entry vector

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. E1_pENTR_L1-L4_NI_G1333-DddtoxA-N

   Plasmid

   #171725

   Purpose

   ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

   Depositor

   Shin-ichi Arimura

   Article

   Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

   Insert

   C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
   

   Use

   Multisite gateway entry vector

   Available Since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. E1_pENTR_L1-L4_NN_G1333-DddtoxA-N

    Plasmid

    #171729

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL1 and L4

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR221 CARD8 FIIND L368G

    Plasmid

    #169969

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (L368G)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Mutation

    L368G

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. E3_pENTR_L3-L2_NG_G1333-DddtoxA-N

    Plasmid

    #171733

    Purpose

    ptpTALECD vector assembly, Entry clone 1 with attL2 and L3

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    C- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
    

    Use

    Multisite gateway entry vector

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221 CARD8 PAM

    Plasmid

    #169975

    Purpose

    pDONR221 for Gateway shuttling. Contains CARD8 (no stop codon) with sgRNA binding site mutations

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Mutation

    PAM

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221 CARD8 FIIND R394E

    Plasmid

    #169971

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (R394E)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Mutation

    R394E

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221 CARD8 FIIND F405G

    Plasmid

    #169973

    Purpose

    pDONR221 for Gateway shuttling. Contains the CARD8 FIIND (L162M-P434, no stop codon) with an interface III mutation (F405G)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Mutation

    F405G

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDONR221 CARD8 FIIND S297A

    Plasmid

    #169966

    Purpose

    pDONR221 for Gateway shuttling. Contains the autoprocessing-deficient CARD8 FIIND (L162M-P434, no stop codon)

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Gateway cloning vector

    Mutation

    S297A

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZA12

    Plasmid

    #158490

    Purpose

    Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V/L17E without STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1 (ZAR1 Mustard Weed)
    

    Use

    Golden gate entry vector

    Mutation

    L17E, D489V, no STOP codon

    Available Since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pYPQ290-D977A

    Plasmid

    #129674

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AacCas12b
    

    Use

    CRISPR; Gateway compatible aaccas12b entry clone

    Expression

    Plant

    Mutation

    D977A; AacCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pYPQ290-E848A

    Plasmid

    #129675

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AacCas12b
    

    Use

    CRISPR; Gateway compatible aaccas12b entry clone

    Tags

    E848A; AacCas12b is rice codon optimized

    Expression

    Plant

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYPQ291-D573A

    Plasmid

    #129676

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    BthCas12b
    

    Use

    CRISPR; Gateway compatible bthcas12b entry clone

    Expression

    Plant

    Mutation

    D573A; BthCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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