We narrowed to 9,676 results for: BLI

1. pJK-caCas9-NatMX-Neut5L-Leu2 drive

   Plasmid

   #89578

   Purpose

   caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

   Depositor

   George Church

   Article

   Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

   Insert

   Leu2 gene drive
   

   Expression

   Yeast

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

   Plasmid

   #218513

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Expression

   Bacterial

   Mutation

   deletion of the Plac_mrfp cassette

   Promoter

   PgyrA_ermBL-ermB'

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 human cofilin S3E

   Plasmid

   #50855

   Purpose

   expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3E (CFL1 Human)
   

   Expression

   Mammalian

   Mutation

   S3E psuedo-phosporylated, non-activatable

   Promoter

   CMV

   Available Since

   Feb. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pQStrep2-CRYM

   Plasmid

   #31293

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   crystallin, mu (CRYM Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQTEV-ASF1A

   Plasmid

   #31591

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   ASF1 anti-silencing function 1 homolog A (S. cerevisiae) (ASF1A Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. HaloTag-3xrGBD

   Plasmid

   #176108

   Purpose

   Visualization of active Rho

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   rhotekin (Rtkn Mouse)
   

   Expression

   Mammalian

   Promoter

   CMVdel

   Available Since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDFNKI-strepII-3C-LIC-strep

   Plasmid

   #108708

   Purpose

   Expression of a StrepII-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Tags

   StrepII-3C protease cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. SNX10-PX (1-201)

   Plasmid

   #119091

   Purpose

   Bacterial expression of human phox homology (PX) domain, SNX10-PX (1-201)

   Depositor

   Brett Collins

   Article

   Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

   Insert

   SNX10-PX (1-201) (SNX10 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28sumo-MDA5-Full-length

   Plasmid

   #210826

   Purpose

   protein expression in ecoli

   Depositor

   Anna Pyle

   Article

   Fitzgerald et al Nucleic Acids Res. 2014 Apr;42(6):3919-3

   Insert

   MDA5 (IFIH1) Full Length Protein (IFIH1 Human)
   

   Expression

   Bacterial

   Mutation

   NA

   Available Since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-HA-mRFP-pcDNA3.1-

    Plasmid

    #52510

    Purpose

    expresses mRFP in mammalian cells with FLAG-HA tag at N-terminus

    Depositor

    Adam Antebi

    Article

    Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

    Insert

    mRFP
    

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    engineered variant of tetrameric red fluorescent …

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

    Plasmid

    #183606

    Purpose

    Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    EGFP-TM
    

    Tags

    HA, Human PDGFRB transmembrane domain, Mouse IgGK…

    Expression

    Mammalian

    Promoter

    Mouse Pgk1

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDH-EF1-FHC-POLQ-DM

    Plasmid

    #64880

    Purpose

    lentiviral expression of human POLQ double mutant (in POL and HDL domains)

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Insert

    POLQ (POLQ Human)
    

    Use

    Lentiviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    K121M and D2330A,Y2331A

    Promoter

    EF1alpha

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUASp YFP Ras85D CA

    Plasmid

    #52247

    Purpose

    Drosophila expression of YFP-Ras85D constitutively active mutant

    Depositor

    Matthew Scott

    Insert

    Ras85D (Ras85D Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q61L; CA mutation

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pETNKI-strepII-3C-LIC-Amp

    Plasmid

    #108707

    Purpose

    Expression of a StrepII-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Tags

    StrepII-3C protease cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR223_HcRed

    Plasmid

    #25892

    Purpose

    Gateway entry vector containing HcRed.

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    hcRed
    

    Use

    Gateway entry vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMXs-hFOXH1

    Plasmid

    #59183

    Purpose

    Forced expression of human FOXH1

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

    Insert

    FOXH1 (FOXH1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. SNX29-PX (269-391)

    Plasmid

    #119108

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX29-PX (269-391)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX29-PX (269-391)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEC3111 (pSpy1C-Plac(3)_ffluc)

    Plasmid

    #218778

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Expression

    Bacterial

    Mutation

    deletion of mrfp cassette, mutagenesis of the RBS…

    Promoter

    Plac(3)

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. p11d-tRPA-32Ala9

    Plasmid

    #102616

    Purpose

    Expression of human RPA with an alanine substituted phosphorylation domain (with the following mutations in RPA32 :S8,11-13,23,29,33,39A,T21A).

    Depositor

    Marc Wold

    Inserts

    RPA1 (RPA1 Human)
    RPA3 (RPA3 )
    RPA2 (RPA2 )
    

    Expression

    Bacterial

    Mutation

    with an alanine substituted phosphorylation domain

    Promoter

    T7

    Available Since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFE-Selo-FI-prk

    Plasmid

    #162698

    Purpose

    Expresses S. elongatus Form IB rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    S. elongatus Form IB rubisco and S. elongatus Prk
    

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Promoter

    PLtet0-1 promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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