We narrowed to 30,836 results for: Ide

1. pXD70LacZ1-promoterless-LacZ

   Plasmid

   #191617

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), promoterless-lacZ

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Expression

   Bacterial

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pdCas9-bacteria

   Plasmid

   #44249

   Purpose

   aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dCas9 (bacteria)
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   pLtetO-1

   Available Since

   April 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCKmatBC

   Plasmid

   #138587

   Purpose

   matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat

   Depositor

   Markus Jeschek

   Article

   Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

   Insert

   bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   matB contains native GUG start codon

   Promoter

   Plac promoter

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEBFP2-Nuc

   Plasmid

   #14893

   Purpose

   Mammalian expression of EBFP2 blue fluorescent protein with NLS

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Insert

   pEBFP2-Nuc
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Replace EYFP of the vector pEYFP-Nuc from Clontec…

   Available Since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. LentiV2-EF1α-nCas9/RT

   Plasmid

   #210188

   Purpose

   Expression of nCas9/RT-P2A-Puro

   Depositor

   Yin Shen

   Article

   Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

   Insert

   nCas9/RT-P2A-Puro
   

   Use

   Lentiviral

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-EF1-FHC

   Plasmid

   #64874

   Purpose

   lentiviral vector with Flag, HA tags

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pP3-GFP

   Plasmid

   #53436

   Purpose

   Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

   Depositor

   Cynthia Collins

   Article

   Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

   Insert

   P3 promoter
   

   Use

   Synthetic Biology

   Tags

   none

   Expression

   Bacterial

   Mutation

   none

   Promoter

   P3

   Available Since

   June 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330-LMNA-gRNA1

   Plasmid

   #122507

   Purpose

   expresses WT spCas9 and a chimeric gRNA targeting human LMNA

   Depositor

   Graham Dellaire

   Article

   Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

   Insert

   LMNA-gRNA1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMH-SFB

   Plasmid

   #99391

   Purpose

   Gateway-compatible mammalian expression destination vector for creating SFB N-terminal fusions (S protein tag, Flag tag, Streptavidin binding peptide)

   Depositor

   Michael Huen

   Article

   An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Type

   Empty backbone

   Tags

   SFB (S protein-FLAG-Streptavidin binding peptide)

   Expression

   Mammalian

   Available Since

   Sept. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-Talin head-SpyCatcher003

    Plasmid

    #133566

    Purpose

    Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    EGFP-Talin head-SpyCatcher003
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICH-chl-roGFP2-synORP1

    Plasmid

    #191701

    Purpose

    Ti plasmid pICH86988 harbouring chl-roGFP2-synORP1 for H2O2 detection in the plastids of plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    chl-roGFP2-synORP1
    

    Use

    Binary agrobacterium ti plasmid

    Expression

    Plant

    Promoter

    CaMV 35S promoter

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRIPZ-shNTC

    Plasmid

    #217638

    Purpose

    Dox inducible shRNA non targeting control shRNA

    Depositor

    Constanze Bonifer

    Article

    Potluri et al Cell Rep. 2021 Apr 20;35(3):109010. doi:

    Insert

    shNTC
    

    Use

    RNAi

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. HcKCR2_mCherry_pcDNA3.1

    Plasmid

    #177337

    Purpose

    Expresses KCR2 in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

    Insert

    HcKCR2
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    mammalian codon-adapted

    Available Since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJM253 (miniCTX1-rhaSR-PrhaBAD)

    Plasmid

    #110571

    Purpose

    Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    rhaSR-PrhaBAD
    

    Expression

    Bacterial

    Promoter

    rhaSR-PrhaBAD

    Available Since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Hsp90 HA

    Plasmid

    #22487

    Depositor

    William Sessa

    Article

    Garcia-Cardena et al Nature. 1998 Apr 23. 392(6678):821-4.

    Insert

    Hsp90 HA (HSP90AB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    May 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmTurquoise2-C1

    Plasmid

    #60560

    Purpose

    Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    mTurquoise2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

    Plasmid

    #138152

    Purpose

    Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    eGFP-MCS
    

    Use

    Lentiviral

    Tags

    2PEST AND NLS

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTi4.0_SpdCas9_pmcDA1

    Plasmid

    #204613

    Purpose

    CRISPR cytosine deaminase integrative plasmid for Mycoplasma gallisepticum

    Depositor

    Pascal Sirand-Pugnet

    Article

    Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. PiggyBac dCas9-VPR

    Plasmid

    #191269

    Purpose

    piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    VPR-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJAK112

    Plasmid

    #167280

    Purpose

    CodA-based mutagenesis vector for C. difficile

    Depositor

    Robert Fagan

    Article

    Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

    Type

    Empty backbone

    Use

    E. coli - c. difficile shuttle vector

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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