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pDONR223_B2M_p.M1V

Plasmid

#81281

Purpose

Gateway Donor vector containing B2M , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

B2M (B2M Human)

Use

Gateway Cloning

Mutation

M1V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RHOA_p.F106L

Plasmid

#81286

Purpose

Gateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RHOA (RHOA Human)

Use

Gateway Cloning

Mutation

F106L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RHOA_p.G62E

Plasmid

#81268

Purpose

Gateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RHOA (RHOA Human)

Use

Gateway Cloning

Mutation

G62E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HIST1H1E_p.A60D

Plasmid

#81269

Purpose

Gateway Donor vector containing HIST1H1E , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HIST1H1E (H1-4 Human)

Use

Gateway Cloning

Mutation

A60D

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_WISP2_p.R230L

Plasmid

#81271

Purpose

Gateway Donor vector containing WISP2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

WISP2 (CCN5 Human)

Use

Gateway Cloning

Mutation

R230L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_B2M_p.M1L

Plasmid

#81274

Purpose

Gateway Donor vector containing B2M , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

B2M (B2M Human)

Use

Gateway Cloning

Mutation

M1L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RHOA_p.Y42S

Plasmid

#81261

Purpose

Gateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RHOA (RHOA Human)

Use

Gateway Cloning

Mutation

Y42S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-A3Ac-puro

Plasmid

#253342

Purpose

Expresses the C-terminal fragment of a rapamycin-inducible split A3A cytosine base editor for in vivo base editing

Depositor

Junwei Shi

Article

Ren et al Nat Biotechnol. 2026 Apr 15. doi: 10.103

Insert

A3Ac

Use

Lentiviral

Tags

3xFLAG-FKBP12-A3Ac-nCas9-puro

Promoter

EFS

Available Since

April 21, 2026

Availability

Academic Institutions and Nonprofits only

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pET29a-hNABP2

Plasmid

#128307

Purpose

Express hNABP2 (a.k.a hSSB1 or SOSSB1) in E. coli with a C-terminal His-tag

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochim Biophys Acta. 2016 Feb;1860(2):3

Insert

Human Nucleic Acid Binding Protein 2 (hNABP2) (NABP2 Human)

Tags

His

Expression

Bacterial

Mutation

Y85C- please see depositor comments below

Promoter

T7

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ255-x3.7

Plasmid

#124311

Purpose

Gateway entry vector with rAPOBEC1 and pcoCas9D10A-x3.7 for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

rAPOBEC1-pcoCas9D10A_x3.7-UGI

Use

CRISPR

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ256-x3.7

Plasmid

#124313

Purpose

Gateway entry vector with PmCDA1 and pcoCas9D10A-x3.7 for C-T base editing

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Insert

pcoCas9D10A_x3.7-PmCDA1-UGI

Use

CRISPR

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR_HA-spGFP11

Plasmid

#240224

Purpose

Gateway entry clone for cloning in insert sequences by gibson assembly to create a C-terminal HA-spGFP11 tag. No ATG start codon.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Type

Empty backbone

Use

Gateway shuttle vector

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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AA197

Plasmid

#216049

Purpose

Fragmid fragment: (C' terminus) ALFA; ALFA; ALFA; ALFA; ALFA; NpNLS; 3xHA

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

ALFA_v1.3; ALFA_v1.2; ALFA_v1.1; ALFA_v1.4; ALFA_v1.5; NpNLS_v1.1; 3xHA_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA375

Plasmid

#216054

Purpose

Fragmid fragment: (C' terminus) HIV nuclear export sequence; HA tag

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

HIV-NES_v1.1; HIV-NES_v1.2; 3xHA_v1.1

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Mouse sgRNA library Brie in lentiCRISPRv2

Pooled Library

#73632

Purpose

Mouse sgRNA library in backbone lentiCRISPRv2 containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
pPB-TetON-mEGFP-HMGB1-WT

Plasmid

#194561

Purpose

Dox-inducible Mammalian Expression of mEGFP-HMGB1 WT IDR Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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ePE2

Plasmid

#202029

Purpose

Prime Editing plasmid for tag insertion

Depositor

Pengcheng Wei

Article

Li et al Plant Commun. 2023 Mar 7:100572. doi: 10

Insert

Cas9，

Use

CRISPR

Expression

Plant

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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CIBN-CAAX

Plasmid

#79574

Purpose

expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

CIBN (CIB1 Mustard Weed)

Tags

C-terminal CAAX-box

Expression

Mammalian

Mutation

K93A, R94A, K106A, K107A (please see depositor co…

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ-CBE-Cas9n-Act3.0

Plasmid

#178955

Purpose

It consists of a Cas9 nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Insert

hA3A-zCas9-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter

Use

CRISPR

Expression

Plant

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFPc1 H-RAS

Plasmid

#236449

Purpose

transient overexpression of H-RAS in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

H-RAS (HRAS Human)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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