We narrowed to 15,009 results for: flag

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NAT-TET-OFF -LEU- 4xFLAG

Plasmid

#182527

Purpose

Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

Depositor

Rob Mitra

Publication

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

7 Copies of TetO Box

Tags

4XFLAG

Expression

Yeast

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV-Drosha (NLS-P426)

Plasmid

#171538

Purpose

Encodes mutant human Drosha protein

Depositor

Yan Zeng

Publication

Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DROSHA (DROSHA Human)

Expression

Mammalian

Mutation

an SV40 NLS sequence (PKKKRKVGI) insertion into t…

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 E144K

Plasmid

#167193

Purpose

puromycin resistant lentiviral vector for expression of PAK5 E144K

Depositor

Christin Burd

Publication

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 E144K

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo G-Linker nsp1

Plasmid

#175426

Purpose

Expresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Publication

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

G-linker nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

G-linker in place of the RNase destabilization do…

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-MPP8-112–858_Flag2-Blast

Plasmid

#175039

Purpose

Mammalian expression of mMPP8 (E112 to Q858) with C-terminal Flag2 tag

Depositor

Kristian Helin

Publication

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Mutation

Expression of MPP8 protein from amino acid positi…

Promoter

CAG

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-2xFLAG-2xSTREP-CCND3-Puro

Plasmid

#172622

Purpose

Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Publication

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND3 (CCND3 Human)

Use

Retroviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

Plasmid

#169166

Purpose

Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

Depositor

John Diffley

Publication

Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

Insert

3xFlag-6His-nsp15 (ORF1ab Synthetic, SARS-CoV-2)

Tags

3xFlag-6His

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 M173I

Plasmid

#167192

Purpose

puromycin resistant lentiviral vector for expression of PAK5 M173I

Depositor

Christin Burd

Publication

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 M173I

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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p8141 MSCV-P C-FlagHA 18E7 D10K

Plasmid

#163306

Purpose

Expresses HPV18 E7 D10K

Depositor

Elizabeth White

Publication

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV18 E7 D10K

Use

Retroviral

Tags

FlagHA

Mutation

D10K

Promoter

MSCV LTR

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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p8126 MSCV-P C-FlagHA 16E7 R77S

Plasmid

#163305

Purpose

Expresses HPV16 E7 R77S

Depositor

Elizabeth White

Publication

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV16 E7 R77S

Use

Retroviral

Tags

FlagHA

Mutation

R77S

Promoter

MSCV LTR

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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p7612 MSCV-P C-FlagHA 16E7 E10K

Plasmid

#163303

Purpose

Expresses HPV16 E7 E10K

Depositor

Elizabeth White

Publication

Hatterschide et al Proc Natl Acad Sci U S A. 2019 Apr 2;116

Insert

HPV16 E7 E10K

Use

Retroviral

Tags

FlagHA

Mutation

E10K

Promoter

MSCV LTR

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-R1868*-3xFLAG

Plasmid

#130679

Purpose

Expresses human SMCHD1 R1868* with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Publication

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 R1868* (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

R1868_V2005delinsX

Promoter

CMV

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-TetOneZeo-ORF18(E36A_L37A)-3xFLAG

Plasmid

#129758

Purpose

Dox inducible expression of KSHV ORF18-3xFLAG (E36A_L37A) in mammalian cells

Depositor

Britt Glaunsinger

Publication

Castaneda et al J Virol. 2018 Oct 10. pii: JVI.01488-18.

Insert

ORF18 (ORF18 Kaposi's sarcoma-associated herpesvirus (HHV-8))

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Glutamic acid 36 to Alanine and Leucine 3…

Promoter

TRE3Gs and Tet inducible promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon37-48-3xFLAG

Plasmid

#130688

Purpose

Expresses human SMCHD1 Exon37-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Publication

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon37-48 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

1M_T1522delinsM

Promoter

CMV

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-36-3xFLAG

Plasmid

#130681

Purpose

Expresses human SMCHD1 Exon1-36 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Publication

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-36 (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D1523_V2005delinsX

Promoter

CMV

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.13/GUG, AAA(30)-FFLuc-3XFLAG

Plasmid

#127335

Purpose

Express 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon (AUG at codon 30 mutated to AAA)

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

SV40

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1APC20-WPRE

Plasmid

#127866

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1APC20 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Publication

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

C-terminal 20 amino acids only

Promoter

hSyn

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG

Plasmid

#127308

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS

Plasmid

#106353

Purpose

CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

Depositor

Stephen Tapscott

Publication

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Insert

D4Z4 gRNA-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSLIK 3XFLAG-MKK7-EE-NLS hygro

Plasmid

#87780

Purpose

Inducible expression of constitutively active mutant MKK7 with a C-terminal NLS sequence

Depositor

Kevin Janes

Publication

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK7 (Map2k7 Mouse)

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only