We narrowed to 58,607 results for: plasmid
-
Viral Prep#105551-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 (#105551). In addition to the viral particles, you will also receive purified pENN.AAV.CamKII.HI.GFP-Cre.WPRE.SV40 plasmid DNA. Expression of GFP-Cre from CamKII promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIITagsGFPAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only
-
pAAV-Ef1a-DIO mScarlet (AAV5)
Viral Prep#131002-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-Ef1a-DIO mScarlet (#131002). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO mScarlet plasmid DNA. EF1a-driven, Cre-dependent mScarlet expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmScarlet (Cre-dependent)Available SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (AAV1)
Viral Prep#198511-AAV1PurposeReady-to-use AAV1 particles produced from pAAV_hSyn-DIO-PdCO-mScarlet-WPRE (#198511). In addition to the viral particles, you will also receive purified pAAV_hSyn-DIO-PdCO-mScarlet-WPRE plasmid DNA. Syn-driven Cre-dependent expression of optimized PdCO in frame with mScarlet. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmScarlet (Cre-dependent)Available SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (AAV8)
Viral Prep#84446-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (#84446). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] plasmid DNA. CAG-driven, Cre-dependent expression of Jaws-KGC-tdTomato-ER2 for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP8s-WPRE (AAV5)
Viral Prep#162374-AAV5PurposeReady-to-use AAV5 particles produced from pGP-AAV-syn-jGCaMP8s-WPRE (#162374). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8s-WPRE plasmid DNA. Syn-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCB-1
Plasmid#210387PurposeAll-in-one cumate-based inducible CRISPRi plasmid for use in StreptomycesDepositorInsertsdCas9
CymR
sgRNA cassette
UseCRISPR; Integrative vector for use in streptomycesMutationBsaI-flanked RFP cassette inserted in place of sp…PromoterSP11 and SP30Available SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE8e-Cas9NG
Plasmid#203328PurposePlasmid for bacterial purification of codon optimized ABE8e-Cas9NGDepositorInsertABE8e-Cas9NG
UseCRISPRTagsHis-tagExpressionBacterialAvailable SinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CBE4max-SpRYc-P2A-EGFP
Plasmid#208336PurposeCAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYcDepositorInserthuman codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
UseCRISPRTagsP2A-EGFPExpressionMammalianMutationSc++ (1-1119), SpRY (1111-1368), D10APromoterCAGAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PE-Max-pet21A
Plasmid#204471PurposeExpression plasmid for PE protein in bacteria.DepositorInsertPrime editor
UseCRISPRTagsHis-tagExpressionBacterialAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
FUGW-ER-TurboID
Plasmid#200965Purpose3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFPDepositorInsertER-TurboID
UseLentiviralTagsEGFPExpressionMammalianAvailable SinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1328 LV EF1a-CD20 IRES-EGFP
Plasmid#201918Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD20DepositorInsertCD20
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKIIa-jGCaMP8m-WPRE (AAV Retrograde)
Viral Prep#176751-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-CamKIIa-jGCaMP8m-WPRE (#176751). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8m-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8m. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCamKIIalphaAvailable SinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKIIa-jGCaMP8f-WPRE (AAV Retrograde)
Viral Prep#176750-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-CamKIIa-jGCaMP8f-WPRE (#176750). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8f-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8f. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCamKIIalphaAvailable SinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLexM-SLC3A2
Plasmid#197994PurposeExpresses SLC3A2 as an N-terminal His tagged construct in mammalian cellsDepositorInsertSLC3A2 (SLC3A2 Human)
TagsHis tagExpressionMammalianMutationPlease see depositor comments belowPromoterCMVAvailable SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-NRCH-PE2max-BSD
Plasmid#191103PurposeLentiviral expression plasmid of NRCH-PEmax prime editor with P2A-BSD markerDepositorInsertNRCH-PEmax
UseCRISPR and LentiviralExpressionMammalianMutationChanged R221K, N394K, H840A from SpCas9-NRCHPromoterEF-1aAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-SpCas9-NRTH-P2A-EGFP (RMD21)
Plasmid#197503PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpCas9-NRTH(D10A) and P2A-EGFPDepositorInsertpCMV-T7-BPNLS-ABE8e-nSpCas9-NRTH-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA and nSpCas9-NRTH(D10A/I32…PromoterCMV and T7Available SinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
T7-VEE-mCherry
Plasmid#193355PurposeSelf-replicating RNA vector expressing mCherry fluorescent protein + PuroRDepositorInsertmCherry
UseVenezuelan equine encephalitis (vee) virus rna re…ExpressionMammalianPromoterT7Available SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-LSSmGFP
Plasmid#186542PurposeLSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmidDepositorInsertLSSmGFP
Tags6xHisExpressionBacterialMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoteraraBAvailable SinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBAD-Lime
Plasmid#191255PurposeBacterial expression plasmids for pH sensitive fluorescent protein LimeDepositorInsertLime
TagsHis tagExpressionBacterialAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
ampl43
Plasmid#161830PurposepRS416-dCas9-Mxi1+TetR+pRPR1(TetO)-NotI-gRNA plasmid marked with His, expressing dCas9-Mxi1 under Tef1 promoter, and a tet-inducible promoter for gRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionYeastPromoterTEF1Available SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only