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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,357 results for: BASE

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Showing: 8241 - 8260 of 13357 results

  3. pUC19 (8nt-gap) (NN48)

    Plasmid
    #257526
    Purpose
    To create an 8nt single-stranded gap in a pUC19-based plasmid by nicking with Nb.BbvCI & Nb.BsmI
    Depositor
    Michael O'Donnell
    Insert
    Nb.BbvCI and Nb.BsmI nicking sites separated by 8 nt
    Use
    Not for gene expression. a dna template for bioch…
    Available Since
    June 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    257526-plasmid-map-sequence-id-512427

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  4. pUC19 (4nt-gap) (NN44)

    Plasmid
    #257524
    Purpose
    To create a 4nt single-stranded gap in a pUC19-based plasmid by nicking with Nb.BbvCI & Nb.BsmI
    Depositor
    Michael O'Donnell
    Insert
    Nb.BbvCI and Nb.BsmI nicking sites separated by 4 nt
    Use
    Not for gene expression. a dna template for bioch…
    Available Since
    June 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    257524-plasmid-map-sequence-id-512370

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  5. pLenti-nSpCas9-APOBEC1

    Plasmid
    #209039
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC1-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209039-plasmid-map-sequence-id-413619

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  9. pFP34∆RBS

    Plasmid
    #247213
    Purpose
    Expresses superfolder GFP (sfGFP) and a Pepper RNA aptamer (tDF30ppr) from a T7 promoter but lacks a ribosomal binding site. Suitable for cell-free expression.
    Depositor
    Elizabeth Strychalski
    Article
    Piorino et al ACS Synth Biol. 2026 Jan 16;15(1):243-26
    Inserts
    superfolder GFP
    Dimeric Pepper RNA aptamer (with tRNA and F30 scaffolds)
    Use
    Synthetic Biology; E. coli-based cell-free expres…
    Tags
    Streptavidin tag
    Expression
    Bacterial
    Mutation
    Two mutations were made to the second Pepper unit…
    Promoter
    T7
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    247213-plasmid-map-sequence-id-507799

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  10. pFP35∆RBS

    Plasmid
    #247214
    Purpose
    Expresses superfolder GFP (sfGFP) and a Pepper RNA aptamer (DF30ppr) from a T7 promoter but lacks a ribosomal binding site. Suitable for cell-free expression.
    Depositor
    Elizabeth Strychalski
    Article
    Piorino et al ACS Synth Biol. 2026 Jan 16;15(1):243-26
    Inserts
    superfolder GFP
    Dimeric Pepper RNA aptamer (with F30 scaffold)
    Use
    Synthetic Biology; E. coli-based cell-free expres…
    Tags
    Streptavidin tag
    Expression
    Bacterial
    Mutation
    Two mutations were made to the second Pepper unit…
    Promoter
    T7
    Available Since
    April 27, 2026
    Availability
    Academic Institutions and Nonprofits only
    247214-plasmid-map-sequence-id-507801

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  14. mCherry-PhoCl AND A-SpyTag

    Plasmid
    #246950
    Purpose
    Expresses multifunctional construct for AND-gated release of mCherry from materials in response to 405 nm light AND sortase eSrtA(2A9) treatment. Contains a SpyTag for material tethering.
    Depositor
    Cole DeForest
    Article
    Ross et al Angew Chem Int Ed Engl. 2025 Nov 10:e199
    Insert
    Please see depositor comments below
    Tags
    6x His tag, SsrA
    Expression
    Bacterial
    Available Since
    Nov. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    246950-plasmid-map-sequence-id-489551

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  15. pTARGEX-ER

    Plasmid
    #242449
    Purpose
    Targets high levels of recombinant protein for retention in ER. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    ER-retrieval motif (KDEL) and Signal peptide deri…
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242449-plasmid-map-sequence-id-489545

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Showing: 8241 - 8260 of 13357 results