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pYPQ141D

Plasmid

#69293

Purpose

Gateway entry vector; single gRNA under OsU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pUCsRNAP

Plasmid

#182746

Purpose

Swapping in small RNA promoter, 23-bp spacer sequence, & 19-bp repeats into pJC005 plasmids

Depositor

James Collins

Article

Chua et al Microbiol Spectr. 2022 Feb 2:e0242721. d

Insert

small RNA promoter, a 23-bp spacer sequence, & 19-bp repeats

Use

CRISPR

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ144

Plasmid

#69296

Purpose

Golden Gate recipient and Gateway entry vector; assembly of 4 gRNAs

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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PJQ49

Plasmid

#134657

Purpose

Contains Promoter 3 with bsa1 cut sites for inserting sgRNA

Depositor

Jason Peters, Oren Rosenberg

Article

Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Anderson BBa_J23115

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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PJQ47

Plasmid

#134646

Purpose

Contains Promoter 1 with bsa1 cut sites for inserting sgRNA

Depositor

Jason Peters, Oren Rosenberg

Article

Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Anderson BBa_J23117

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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PJQ48

Plasmid

#134647

Purpose

Contains Promoter 2 with bsa1 cut sites for inserting sgRNA

Depositor

Jason Peters, Oren Rosenberg

Article

Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

Anderson BBa_J23114

Available Since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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p2CT-Cdi

Plasmid

#83044

Purpose

Expresses Corynebacterium diphtheriae Cas9 (CdiCas9)

Depositor

Jennifer Doudna

Article

Ma et al Mol Cell. 2015 Nov 5;60(3):398-407. doi:

Insert

CdiCas9

Tags

His-MBP

Expression

Bacterial

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only
pJ2018

Plasmid

#140665

Purpose

dCas9, LuxR & Csy4

Depositor

Yolanda Schaerli

Article

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

dCas9, LuxR & Csy4

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pdCas9- AsiA_m2.1

Plasmid

#158065

Purpose

Expressing evolved CasTA of dCas9 fused AsiA variant 2.1

Depositor

Harris Wang

Article

Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

Insert

dCas9-AsiA_m2.1

Expression

Bacterial

Promoter

TetO

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-Zeo-BsmBI_entry (pRW1500)

Plasmid

#225753

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with Zeocin resistance; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pRB1017

Plasmid

#59936

Purpose

empty vector for gRNA cloning

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Type

Empty backbone

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAW014-2

Plasmid

#85612

Purpose

Multi-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

ugtP-gRNA.P395T

Expression

Bacterial

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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hCtRNA_FT

Plasmid

#186715

Purpose

Fragment template for DAP array

Depositor

Xue Gao

Article

Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

Insert

gRNA scaffold and hCtRNA

Use

CRISPR

Expression

Mammalian

Promoter

hCtRNA

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgTh

Plasmid

#159901

Purpose

Mutagenesis of Th

Depositor

Larry Zweifel

Insert

Th (Th Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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hCtRNA_VT

Plasmid

#186716

Purpose

Vector template for DAP array

Depositor

Xue Gao

Article

Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

Insert

hCtRNA and gRNA scaffold

Use

CRISPR

Expression

Mammalian

Promoter

hCtRNA

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pEG302-SunTagng-22aa

Plasmid

#115345

Purpose

Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome

Depositor

Steven Jacobsen

Insert

NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ141B

Plasmid

#69291

Purpose

Gateway entry vector; single gRNA under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA-Adrb1

Plasmid

#184294

Purpose

A single vector containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA targeting Adrb1

Depositor

Bernardo Sabatini

Insert

sgRNA-Adrb1

Use

AAV and CRISPR

Expression

Mammalian

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ141C

Plasmid

#69292

Purpose

Gateway entry vector; single gRNA under OsU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEXfrt-SaCas9-U6-sgRosa26

Plasmid

#159915

Purpose

Mutagenesis of Rosa26

Depositor

Larry Zweifel

Insert

Rosa26 (Gt(ROSA)26Sor Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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