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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,841 results for: BLI;

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Showing: 8301 - 8320 of 11841 results

  1. pEmal10H – MBP-EWS_549-638 (RGG3)

    Plasmid
    #238370
    Purpose
    Expresses the RGG3 (549-638) region from the RNA-binding protein EWS
    Depositor
    David Libich
    Insert
    EWSR1 (EWSR1 Human)
    Tags
    10xHis-MBP
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-548
    Promoter
    T7
    Available Since
    Sept. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    238370-plasmid-map-sequence-id-478744

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  4. NanoLuc-TAF1(BD2) Fusion Vector

    Plasmid
    #237201
    Purpose
    Express NanoLuc(R)-TAF1(BD2) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    TAF1 (TAF1 Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    BD2 domain only (residue 1515-1669)
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237201-plasmid-map-sequence-id-476793

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  5. EIF2AK2(252-551)-NanoLuc Fusion Vector

    Plasmid
    #237172
    Purpose
    Express EIF2AK2(252-551)-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    EIF2AK2 (EIF2AK2 Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    Truncation of full-length protein; expressing res…
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237172-plasmid-map-sequence-id-476789

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  6. LgBiT-KRAS 4B(G12V) BiBiT-Ready Vector

    Plasmid
    #237076
    Purpose
    Express LgBiT-KRAS 4B(G12V) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    G12V
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237076-plasmid-map-sequence-id-471923

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  7. LgBiT-KRAS 4B G12C BiBiT Ready Vector

    Plasmid
    #237078
    Purpose
    Express LgBiT-KRAS 4B G12C Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    G12C
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237078-plasmid-map-sequence-id-471925

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  8. HiBiT-KRAS(G12C) Fusion Vector

    Plasmid
    #236889
    Purpose
    Express HiBiT-KRAS(G12C) in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    HiBit
    Expression
    Mammalian
    Mutation
    G12C
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236889-plasmid-map-sequence-id-472710

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  9. NanoBRET Assay Vector, HaloTag-KRAS

    Plasmid
    #236871
    Purpose
    Express HaloTag(R)-KRAS in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236871-plasmid-map-sequence-id-473394

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  10. NanoBRET Assay Vector, NanoLuc-NRAS(Q61K)

    Plasmid
    #236854
    Purpose
    Express NanoLuc(R)-NRAS(Q61K) in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    NRAS (NRAS Human)
    Tags
    HaloTag (R) and NanoLuc (R)
    Expression
    Mammalian
    Mutation
    Q61K
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236854-plasmid-map-sequence-id-477412

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  11. NanoBRET Assay Vector, HaloTag-KRAS(G13D)

    Plasmid
    #236856
    Purpose
    Express HaloTag(R)-KRAS(G13D) in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    G13D
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236856-plasmid-map-sequence-id-471852

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  12. SmBiT-KRAS4B(G12D) BiBiT-Ready Vector

    Plasmid
    #236798
    Purpose
    Express SmBiT-KRAS4B(G12D) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS4B(G12D) (KRAS Human)
    Tags
    SmBiT
    Expression
    Mammalian
    Mutation
    G12D
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236798-plasmid-map-sequence-id-473530

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  13. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid
    #239448
    Purpose
    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.
    Depositor
    Alexis Komor
    Inserts
    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    BFP: T65S, S72S, V145F, K206K, H231L
    Promoter
    EF1alpha, U6, and mU6
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239448-plasmid-map-sequence-id-476590

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  14. rTetR-GFP-p65

    Plasmid
    #232633
    Purpose
    p65 activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition
    Depositor
    Karsten Rippe
    Insert
    rTetR-GFP-p65 (RELA Human, Synthetic)
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232633-plasmid-map-sequence-id-463626

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  15. Kif3A-EGFP-SNAP

    Plasmid
    #229852
    Purpose
    Kif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experiments
    Depositor
    William Hancock
    Insert
    Kif3A (Kif3a Mouse)
    Tags
    6xHis, EGFP, and SNAP-tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229852-plasmid-map-sequence-id-460709

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  16. pLANT-2b

    Plasmid
    #231487
    Purpose
    Expresses His6-SUMO^NIT-FraB where "NIT" stands for "no internal translation", reflecting the mutation of the two Shine-Dalgarno-like sequences within the SUMO tag.
    Depositor
    Venkat Gopalan
    Inserts
    SUMO^NIT (SMT3 Budding Yeast)
    FraB
    Tags
    His6 tag and His6-SUMO^NIT
    Expression
    Bacterial
    Mutation
    Deleted amino acids 1-6 (MEPEES). and M83L, E84Q,…
    Promoter
    T7 promoter
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    231487-plasmid-map-sequence-id-460673

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  17. E547* FL USP7

    Plasmid
    #229001
    Purpose
    E547-mutant of human USP7
    Depositor
    Irina Bezsonova
    Insert
    USP7 1-546 (USP7 Human)
    Tags
    6xHis cleavable with thrombin
    Expression
    Bacterial
    Mutation
    E547 mutated to stop codon
    Promoter
    T7
    Available Since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    229001-plasmid-map-sequence-id-458328

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  18. pCAGGS-Twin-strep-TEV-DGKδ2

    Plasmid
    #223720
    Purpose
    Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    DGKD (DGKD Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    deleted start codon
    Promoter
    CAG and chicken β-actin promoter
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    223720-plasmid-map-sequence-id-457431

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  19. pCAGGS-PHOSPHO1-TEV-Twin-Strep

    Plasmid
    #222015
    Purpose
    Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell
    Depositor
    Chiaki Murakami
    Insert
    PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
    Tags
    TEV cleavable site and Twin-Strep-tag
    Expression
    Mammalian
    Mutation
    stop codon (TGA) is deleted
    Promoter
    chicken β-actin promoter
    Available Since
    Jan. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    222015-plasmid-map-sequence-id-457427

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Showing: 8301 - 8320 of 11841 results