Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 28,294 results for: cmv promoter

Clear keywords
Showing: 8321 - 8340 of 28294 results

  3. PSB-CMV-Puro ArfGAP1_ALPS1_ALPS2-mKate2-3NLS-P2A-EGFP-Sec61B

    Plasmid
    #250913
    Purpose
    Dual color expression of mKate2-3NLS tagged human ARFGAP1 ALPS1-2 and EGFP tagged human Sec 61B in mammalian cell lines
    Depositor
    Philipp Niethammer
    Article
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-3xNLS-P2A-EGFP-Sec61B (SEC61B, ARFGAP1 Human)
    Tags
    EGFP on Sec61b and mKate2-3xNLS on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 192–304 only
    Promoter
    CMV
    Available Since
    Feb. 17, 2026
    Availability
    Academic Institutions and Nonprofits only
    250913-plasmid-map-sequence-id-498650

    Enlarge

  4. PSB-CMV-Puro ArfGAP1_ALPS1-mkate2-3NLS-P2A-EGFP-Sec61B

    Plasmid
    #250912
    Purpose
    Dual color expression of mKate2-3NLS tagged rat ARFGAP1 ALPS1 and EGFP tagged human Sec 61B in mammalian cell lines
    Depositor
    Philipp Niethammer
    Article
    Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/
    Insert
    ArfGAP1-mKate2-3xNLS-P2A-EGFP-Sec61B (Arfgap1, SEC61B Rat, Human)
    Tags
    EGFP on Sec61B and mKate2-3xNLS on ArfGAP1
    Expression
    Mammalian
    Mutation
    ARFGAP1 amino acids 196–234 only
    Promoter
    CMV
    Available Since
    Jan. 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250912-plasmid-map-sequence-id-498488

    Enlarge

  5. pCMV-T7-BPNLS-CE-ABE8e-SpCas9(D10A)-BPNLS-P2A-EGFP (NK350)

    Plasmid
    #208292
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized CE-ABE8e-SpCas9 A-to-G base editor and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Insert
    pCMV-T7-BPNLS-CE-ABE8e-SpCas9-BPNLS-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA inlaid into nSpCas9(D10A)
    Promoter
    CMV and T7
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    208292-plasmid-map-sequence-id-410847

    Enlarge

  8. pCMV-T7-nSpRY(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES395)

    Plasmid
    #185489
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpRY(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpRY(H840A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    H840A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N13…
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185489-plasmid-map-sequence-id-362674

    Enlarge

  9. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

    Plasmid
    #185490
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    H840A
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185490-plasmid-map-sequence-id-362676

    Enlarge

  10. pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)

    Plasmid
    #185491
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185491-plasmid-map-sequence-id-362675

    Enlarge

  11. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-M-MLV(PE2) (JO586)

    Plasmid
    #217800
    Purpose
    Variant CE1 construct with engineered M-MLV reverse transcriptase (RT; found in PE2), expressed from CMV or T7 promoters
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-M-MLV(PE2)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); PE2 M-MLV(D200N/L603W/T330P/T306K…
    Promoter
    CMV and T7
    Available Since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217800-plasmid-map-sequence-id-432875

    Enlarge

  17. pCMV CHMP2Bchimera-L4D,F5D-CHMP6α2-3XHA

    Plasmid
    #242419
    Purpose
    Expression of a CHMP2B membrane binding mutant (L4D F5D) with the second alpha helix (residues 55-96) replaced by that from CHMP6 (residues 57-98) and a 3xHA tag.
    Depositor
    Franz-Ulrich Hartl
    Article
    Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e
    Inserts
    CHMP2B (CHMP2B Human)
    CHMP6 (CHMP6 Human)
    Tags
    3xHA
    Expression
    Mammalian
    Mutation
    L4D F5D; Helix 2 (residues 55-96) replaced with r…
    Promoter
    CMV
    Available Since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    242419-plasmid-map-sequence-id-500686

    Enlarge

  18. Lenti-CMV-EGFP-MTHFD1 K56R-WPRE

    Plasmid
    #250371
    Purpose
    Lentiviral expression of human MTHFD1 carrying K56R mutation with N-terminal EGFP and puromycin resistance cassette under PGK promoter. Mutation was generated by Q5 site-directed mutagenesis.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    MTHFD1 (MTHFD1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    K56R
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250371-plasmid-map-sequence-id-498985

    Enlarge

  19. Lenti-CMV-EGFP-MTHFD1 K384E-WPRE

    Plasmid
    #250372
    Purpose
    Lentiviral expression of human MTHFD1 carrying K384E mutation with N-terminal EGFP and puromycin resistance cassette under PGK promoter. Mutation was generated by Q5 site-directed mutagenesis.
    Depositor
    Stefan Kubicek
    Article
    Nguyen et al Science. 2025 Nov 6:eadv4257. doi: 10.11
    Insert
    MTHFD1 (MTHFD1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    K384E
    Promoter
    CMV
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    250372-plasmid-map-sequence-id-498984

    Enlarge

Showing: 8321 - 8340 of 28294 results