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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 15,542 results for: LEA

Showing: 8321 - 8340 of 15542 results

  1. pKP2189

    Plasmid
    #167644
    Purpose
    Express FepA T17C, I14P . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Insert
    fepA T17C, I14P
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fepA with T17C, I14P mutations (Mutations are I36…
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167644-plasmid-map-sequence-id-339582

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  2. pKP2185

    Plasmid
    #167635
    Purpose
    Express FepA D12C, I14P . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Insert
    fepA D12C, I14P
    Use
    Tags
    Expression
    Bacterial
    Mutation
    fepA with D12C, I14P mutations (Mutations are D34…
    Promoter
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    167635-plasmid-map-sequence-id-339489

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  3. pKP2188

    Plasmid
    #167642
    Purpose
    Express FepA V16C, I14P . Please note that mutation numbers are not based on start codon of insert.
    Depositor
    Kathleen Postle
    Insert
    fepA V16C, I14P
    Use
    Tags
    Expression
    Bacterial
    Mutation
    FepA with V16C, I14P mutations. (They are numbere…
    Promoter
    Available since
    July 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    167642-plasmid-map-sequence-id-337262

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  4. T2m

    Plasmid
    #121029
    Purpose
    MoClo golden gate assembly DE part for Eck120033736 (164.6x-strength natural terminator; see DOI: 10.1038/nmeth.2515) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    ECK120033736 (164.6x Voigt terminator), short attachment
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121029-plasmid-map-sequence-id-238076

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  5. T7m

    Plasmid
    #121030
    Purpose
    MoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    S. pyogenes tracrRNA terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121030-plasmid-map-sequence-id-236182

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  6. T13m

    Plasmid
    #121032
    Purpose
    MoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ECK120033737 (second strongest natural Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121032-plasmid-map-sequence-id-236342

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  7. C100m

    Plasmid
    #121004
    Purpose
    MoClo golden gate assembly CD part sfYFP-ptd3 (superfolder YFP with pdt3 degradation tag for mfLon). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121004-plasmid-map-sequence-id-236143

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  8. C41m

    Plasmid
    #120974
    Purpose
    MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    dCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120974-plasmid-map-sequence-id-236028

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  9. C61m

    Plasmid
    #120977
    Purpose
    MoClo golden gate assembly CD part for RpaR* (improved RpaR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    RpaR from Scott and Hasty 2016
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120977-plasmid-map-sequence-id-236031

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  10. C62m

    Plasmid
    #120978
    Purpose
    MoClo golden gate assembly CD part for TraR(W) (improved TraR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    TraR(W) from Scott and Hasty 2016
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120978-plasmid-map-sequence-id-236032

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  11. P12m

    Plasmid
    #120938
    Purpose
    MoClo golden gate assembly AB part for pLas-TetO (Promoter activated by LasR and repressed by TetR; encodes <3OC12 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pLasTetO
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120938-plasmid-map-sequence-id-256615

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  12. P15m

    Plasmid
    #120939
    Purpose
    MoClo golden gate assembly AB part for pCin-TetO (Promoter activated by CinR and repressed by TetR; encodes <3OC14 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pCinTetO
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120939-plasmid-map-sequence-id-238035

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  13. P16m

    Plasmid
    #120940
    Purpose
    MoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes <3OC14 AHL AND IPTG> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ohAB carb pCinLacO m
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120940-plasmid-map-sequence-id-238036

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  14. P32m

    Plasmid
    #120943
    Purpose
    MoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    PlacO (Tim Lu, Matt Bennett, etc)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120943-plasmid-map-sequence-id-236887

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  15. P33m

    Plasmid
    #120944
    Purpose
    MoClo golden gate assembly AB part (medium-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    P11 from Mutalik library (medium)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120944-plasmid-map-sequence-id-245421

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  16. P34m

    Plasmid
    #120945
    Purpose
    MoClo golden gate assembly AB part (low-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    P13 from Mutalik library (weak)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120945-plasmid-map-sequence-id-238039

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  17. pX330-Flag-HFSpCas9-H840A

    Plasmid
    #80455
    Purpose
    high fidelity SpCas9 mammalian expression vector
    Depositor
    Ervin Welker
    Insert
    HFSpCas9_H840A
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80455-plasmid-map-sequence-id-151476

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  18. 5CBD

    Plasmid
    #69970
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PVX_084705:MAC04G-C11:C228845
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Expression
    Mutation
    Promoter
    polyhedrin
    Available since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. 5C4Q

    Plasmid
    #69963
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    LdBPK_363130.1:MAC055-F03:C235029
    Use
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Available since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 8321 - 8340 of 15542 results