Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 9,247 results for: Arch

Showing: 8341 - 8360 of 9247 results

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pETM33_Nsp3c_SUD-M

Plasmid

#156470

Purpose

Bacterial expression of Sars-CoV2 Nsp3c_SUD-M protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3c_SUD-M (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4b)

Plasmid

#241044

Purpose

Expresses SARS-CoV-2 spike (B.1-4b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S494R,K529L,D614G,R683L

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4c)

Plasmid

#241045

Purpose

Expresses SARS-CoV-2 spike (B.1-4c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

N439R,T478K,E484V,D614G,K529L

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4a)

Plasmid

#241042

Purpose

Expresses SARS-CoV-2 spike (B.1-4a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,S494R,F490R,D614G,R683N

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-4d)

Plasmid

#241043

Purpose

Expresses SARS-CoV-2 spike (B.1-4d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-4d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T76I,M153S,Y473F,Q493R,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-2c)

Plasmid

#241040

Purpose

Expresses SARS-CoV-2 spike (B.1-2c) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2c) (S SARS-CoV-2)

Expression

Mammalian

Mutation

R346T,K417V,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-2d)

Plasmid

#241041

Purpose

Expresses SARS-CoV-2 spike (B.1-2d) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2d) (S SARS-CoV-2)

Expression

Mammalian

Mutation

K147N,K417V,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-2a)

Plasmid

#241038

Purpose

Expresses SARS-CoV-2 spike (B.1-2a) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2a) (S SARS-CoV-2)

Expression

Mammalian

Mutation

T478K,R346T,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1-2b)

Plasmid

#241039

Purpose

Expresses SARS-CoV-2 spike (B.1-2b) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1-2b) (S SARS-CoV-2)

Expression

Mammalian

Mutation

R346T,S494R,D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDMJ2-SARS-CoV-2-Spike (B.1)

Plasmid

#241033

Purpose

Expresses SARS-CoV-2 spike (B.1) protein for creating pseudotyped virus

Depositor

Jeremy Luban

Article

Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

Insert

Spike (B.1) (S SARS-CoV-2)

Expression

Mammalian

Mutation

D614G

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SSH-pSUMO-β′-COPI-WD40-Lys17Ala

Plasmid

#208171

Purpose

Bacterial expression plasmid for Saccharomyces cerevisiae β′-COPI-WD40-Lys17Ala mutant (residues 1-301)

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

N-terminal domain of yeast β′-COPI-WD40 subunit (1-301) with Lys17Ala substitution

Tags

His-tag, SUMO-tag, and Strep-tag

Expression

Bacterial

Mutation

Lys17Ala substitution

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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BD-pGEX5X3-BD-SARS-CoV-2-S-tail-WT

Plasmid

#218455

Purpose

Bacterial expression plasmid for the SARS-CoV-2 cytosolic tail with an N-terminal GST tag

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

Cytosolic tail of SARS-CoV-2 spike carrying an N-terminal GST-tag (S SARS-CoV-2)

Tags

GST

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 E484Q (CAA)

Plasmid

#212423

Purpose

Encodes SARS-CoV-2 spike E484Q for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike E484Q (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

E484Q

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 L981F (TTC)

Plasmid

#212486

Purpose

Encodes SARS-CoV-2 spike L981F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L981F (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L981F

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 T572I (ATC)

Plasmid

#212404

Purpose

Encodes SARS-CoV-2 spike T572I for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T572I (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T572I

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 T29I (ATA)

Plasmid

#212403

Purpose

Encodes SARS-CoV-2 spike T29I for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike T29I (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

T29I

Promoter

CAG

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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AP2u2-CMV-S_protein

Plasmid

#226456

Purpose

Mammalian expression of S protein of SARS-CoV2 under CMV promoter, tagged with 6His and released extracellular environment after expression for protein purification

Depositor

Urartu Seker

Article

Ozcelik et al ACS Pharmacol Transl Sci. 2024 Mar 21;7(

Insert

Spike Protein

Tags

6xHis Tag, Foldon Domain, and Thrombin Cleavage S…

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 V1104L (TTA)

Plasmid

#223274

Purpose

Encodes SARS-CoV-2 spike V1104L for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike V1104L (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

V1104L

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Y453F (TTC)

Plasmid

#212369

Purpose

Encodes SARS-CoV-2 spike Y453F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Y453F (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

Y453F

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 BA.5

Plasmid

#212505

Purpose

Encodes SARS-CoV-2 variant BA.5 Spike for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike BA.5 (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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