We narrowed to 8,939 results for: pcDNA3

Showing: 8341 - 8360 of 8939 results

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AcrVA1_cpGR2(GGS5)-S103ΔELS

Plasmid

#246050

Purpose

Expression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS; enables chemogenetic control of MbCas12a

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrVA1_cpGR2-S103ΔELS

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔELS

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_AcrIIA4_cpGR2(GGS5)-ΔN64_Q65_E66

Plasmid

#246052

Purpose

Expression of AcrIIA4-cpGR2 hybrid in mammalian cells after Bubeck et al Nat Methods 2018; enables chemogenetic control of SpyCas9

Depositor

Dominik Niopek

Article

Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

Insert

AcrIIA4_cpGR2-ΔN64/Q65/E66

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Mutation

ΔN64/Q65/E66

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHCFnc/V5

Plasmid

#247003

Purpose

Expresses non cleavable HCF-1 with carboxy terminal V5 tag under control of the CMV promoter

Depositor

Thomas Kristie

Article

Vogel et al Proc Natl Acad Sci U S A. 2006 May 2;103

Insert

HCF-1 (HCFC1 Human)

Tags

V5 tag

Expression

Mammalian

Mutation

Glu to Ala change at amino acid positions 1019,10…

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-T453D

Plasmid

#235209

Purpose

Expresses GRP78 with T453D mutation in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Change Threonine 453 to Aspartic Acid

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-R197H

Plasmid

#235208

Purpose

Expresses GRP78 with R197H mutation in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Change Arginine 197 to Histidine

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-G227D

Plasmid

#235207

Purpose

Expresses GRP78 with G227D mutation in mammalian cells

Depositor

Amy Shiu Lee

Article

Tsai et al J Biol Chem. 2015 Mar 27;290(13):8049-64

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Change Glycine 227 to Aspartic Acid

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-NLS-Mut

Plasmid

#235206

Purpose

Expresses GRP78 with mutated NLS in mammalian cells

Depositor

Amy Shiu Lee

Article

Liu et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Mutate the NLS sequence

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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mCGN-Myc-His

Plasmid

#236510

Purpose

For expression of Cingulin (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGN (Cgn Mouse)

Tags

Myc-His

Expression

Mammalian

Mutation

WT

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGN-Myc

Plasmid

#236509

Purpose

For expression of Cingulin (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGN (Cgn Mouse)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGNL1

Plasmid

#236519

Purpose

For expression of Paracingulin (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGNL1 (Cgnl1 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-cCGN-Myc

Plasmid

#236506

Purpose

For expression of CGN (dog) in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGN (CGN Canis lupus familiaris (dog))

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCGN-mCGNL1-Rod1-Myc

Plasmid

#236526

Purpose

For expression of chimeric CGN containing the Rod1 domain from CGNL1 (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Flinois et al J Cell Sci. 2024 Mar 1;137(5):jcs260745.

Insert

CGN-CGNL1 (Cgn Mouse)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(1-573)

Plasmid

#219683

Purpose

Mammalian expression of CLIPf-KIF5A(1-573)

Depositor

Alison Twelvetrees

Article

Smith et al iScience. 2026 Feb 2;29(3):114875. doi:

Insert

KIF5A (Kif5a Mouse)

Tags

CLIPf

Expression

Mammalian

Mutation

1-573

Promoter

CMV & T7

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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hA3A*-eIscBn-D

Plasmid

#227147

Purpose

Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant and HMG-D domain was inserted between hA3A* and eIscBn driven by CMV promoter

Depositor

Dali Li

Article

Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e

Insert

bpNLS-hA3A*-linker-HMG-D-linker-nOgeuIscB-v3-nucleoplasmin NLS-2XUGI-bpNLS

Mutation

D96R/E84R/V159R/H339A

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

Plasmid

#191572

Purpose

Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

SARS-CoV-2 Spike Protein (S Synthetic)

Tags

3x FLAG

Expression

Mammalian

Mutation

D614G and F486S (confers reduced binding affinity…

Promoter

CMV

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Kv2.1 K+ channel scFv [D4/154]

Plasmid

#206787

Purpose

Mammalian Expression of Kv2.1 K+ channel scFV. Derived from hybridoma D4/154 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv2.1 K+ channel recombinant scFV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-Δ220-234-Flag

Plasmid

#210341

Purpose

Expresses SARS-CoV-2 NSP4-Δ220-234 fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

Flag

Expression

Mammalian

Mutation

Δ220-234

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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NSP4-H120N/F121L-Flag

Plasmid

#210340

Purpose

Expresses SARS-CoV-2 NSP4-H120N/F121L fused with Flag in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)

Tags

Flag

Expression

Mammalian

Mutation

H120N/F121L

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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CCM2_d410-444_eGFP

Plasmid

#208877

Purpose

Express eGFP-CCM2 lacking amino acids 410-444 in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

CCM2 (CCM2 Human)

Tags

eGFP

Expression

Mammalian

Mutation

amino acids deletion : 410-444

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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KCC2 scFv [N1/12]

Plasmid

#190520

Purpose

Mammalian Expression of KCC2 scFV. Derived from hybridoma N1/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

KCC2 (Rattus norvegicus) recombinant scFV (Slc12a5 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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