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pBEL2060

Plasmid

#162146

Purpose

Expression plasmid for MlaE Val77Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Val77Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2061

Plasmid

#162147

Purpose

Expression plasmid for MlaE Leu78Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Leu78Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2062

Plasmid

#162148

Purpose

Expression plasmid for MlaE Leu70Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Leu70Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2063

Plasmid

#162149

Purpose

Expression plasmid for MlaE Leu99Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Leu99Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2065

Plasmid

#162150

Purpose

Expression plasmid for MlaE Trp149Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Trp149Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2066

Plasmid

#162151

Purpose

Expression plasmid for MlaE Phe209Bpa mutant for in vivo crosslinking

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE, Phe209Bpa)-MlaD-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2139

Plasmid

#162132

Purpose

Expression and Complementation plasmid for MlaD TM swap with LptC TM

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(TMdeltaLptC TM)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2138

Plasmid

#162133

Purpose

Expression and Complementation plasmid for MlaD TM swap with LetB TM

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(TMdeltaLetB TM)-MlaC-MlaB

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT083

Plasmid

#180736

Purpose

Markerless yeast integration Vector at Int.9 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT084

Plasmid

#180737

Purpose

Markerless yeast integration Vector at Int.10 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT081

Plasmid

#180734

Purpose

Markerless yeast integration Vector at Int.7 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT080

Plasmid

#180733

Purpose

Markerless yeast integration Vector at Int.6 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT079

Plasmid

#180732

Purpose

Markerless yeast integration Vector at Int.5 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT078

Plasmid

#180731

Purpose

Markerless yeast integration Vector at Int.4 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT077

Plasmid

#180730

Purpose

Markerless yeast integration Vector at Int.3 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT075

Plasmid

#180728

Purpose

Markerless yeast integration Vector at Int.1 locus to used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT022

Plasmid

#180675

Purpose

Encodes mScarlet-I as a Type 4a part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

mScarlet-I

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT019

Plasmid

#180672

Purpose

Encodes mScarlet-I as a Type 3 part to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

mScarlet-I

Use

Synthetic Biology

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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p-sfGFP-pA-FRT-Amp-FRT

Plasmid

#176620

Purpose

for generating PCR templates with sfGFP and Amp for BAC recombination

Depositor

Nathan Lawson

Article

Shih et al Development. 2021 Nov 9. pii: 273393. do

Insert

super folder green fluorescent protein with FRT-flanked ampicillin cassette

Use

Unspecified

Mutation

H217R - please see dep. comments

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2298

Plasmid

#164603

Purpose

Expression and Complementation plasmid for MlaD (A17F, A20F, V24F)

Depositor

Gira Bhabha, Damian Ekiert

Article

Coudray et al Elife. 2020 Nov 25;9. pii: 62518. doi: 1

Insert

MlaFEDCB

Expression

Bacterial

Mutation

MlaF-(6xHis-MlaE)-MlaD(Ala17Phe, Ala20Phe, Val24P…

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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