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pJH76

Plasmid

#172144

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.2TAG, luxAB; Plpp pylS; PproK pylT

Expression

Bacterial

Mutation

gIII(P29*, Y184*)

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-EGFP-RanGAP

Plasmid

#175236

Purpose

Bacterial expression and purification, high affinity SUMOylation substrate that can recruited to FRB with rapamycin

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

RANGAP1 (RANGAP1 Human)

Expression

Bacterial

Promoter

tac

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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DDX41_p.L237F_p.P238T_EGFP

Plasmid

#175845

Purpose

Expresses DDX41 with mutation

Depositor

Evan Braunstein

Article

Li et al Haematologica. 2016 Jun;101(6):e228-31.

Insert

DDX41 (DDX41 Human)

Tags

EGFP

Expression

Mammalian

Mutation

c.711G>T, p.L237F and c.712C>A, p.P238T

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pJH858

Plasmid

#172176

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.3f2deopt]

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH859

Plasmid

#172177

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1deopt]

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBdeopt(N61S…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH578

Plasmid

#172162

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 3f2_v2); AraC

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH580

Plasmid

#172164

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 3f2v2+B); AraC

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K, E178K, G188R, E251D, G332…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH581

Plasmid

#172165

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 3f2+C); AraC

Expression

Bacterial

Mutation

PylB(N61S, E84K, E122K, E178K, G188R, E251D) PylC…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH750

Plasmid

#172170

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.3f2, deleted sumo

Expression

Bacterial

Mutation

Evolved PylBCD operon; SUMO tag deleted from PylB…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH751

Plasmid

#172171

Purpose

Microscopy inclusion body assay

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

pCDF.JM10.1, deleted sumo

Expression

Bacterial

Mutation

Evolved PylBCD operon; SUMO tag deleted from PylB…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH767

Plasmid

#172172

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.WT]

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB]

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH768v2

Plasmid

#172173

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.3f2]

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH769v2

Plasmid

#172174

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.JM10.1]

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH857

Plasmid

#172175

Purpose

PylB overexpression and purification

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/Lac-[His6-TEV-SUMO-PylB.WTopt]

Expression

Bacterial

Mutation

pET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylBopt]

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH191

Plasmid

#172149

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.2TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT

Expression

Bacterial

Mutation

gIII(P29*, Y184*); [strong promoter eG, strong RB…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH202

Plasmid

#172150

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.1TAG, luxAB; Plpp 32A; PproK pylT

Expression

Bacterial

Mutation

gIII(P29*); 32A pylS variant

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH223

Plasmid

#172151

Purpose

pylBCD variant in pCDF backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/lac (pylBCD 34B_sub-pop5); LacI

Expression

Bacterial

Mutation

PylB(N61S, E178K, E251D) PylD(A252V)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH224

Plasmid

#172152

Purpose

pylBCD variant in pCDF backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

PT7/lac (pylBCD 40C_sub-pop6); LacI

Expression

Bacterial

Mutation

PylB(N61S, E175K, E178K)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH463

Plasmid

#172155

Purpose

pylBCD variant in pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Para (pylBCD 36A_sub-pop1); AraC

Expression

Bacterial

Mutation

PylB(N61S, E122K, E178K, G188R)

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH572

Plasmid

#172156

Purpose

Negative control pBAD backbone

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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