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pcDNA3.2 mir-1-1 reporter mir16-1 wt
Plasmid#46683PurposeExpresses wild-type miR-16-1 in mammalian cellsDepositorInsertmir16-1 wt (MIR16-1 Human, Synthetic)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianMutationPromoterCMVAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVRa28_1088
Plasmid#49683DepositorInsertECF28_1088
UseSynthetic BiologyTagsHis6-PreScissionExpressionBacterialMutationCodon optimized for E.coliPromoterpT7-lacOAvailable sinceJan. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-plk4
Plasmid#136837PurposeMammalian expression of the centrosomal protein plk4 N-terminally fused to CLIP-tagDepositorInsertCLIP-plk4 (PLK4 Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 NLRP1 FIIND (F1079M-L1373) S1213A
Plasmid#166832PurposeGateway pDONR vector containing NLRP1 FIIND with autoprocessing deficient S1213A mutation (no stop codon)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsExpressionMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV hGRN2
Plasmid#213683PurposeExpresses human granulin-2 with an N-terminal twin-Strep-FLAG tagDepositorInserthuman Granulin-2
UseAAVTagstwin-Strep-FLAGExpressionMutationPromotercytomegalovirus enhancer/chicken β-actin promoterAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRB3 CTF-FLAG
Plasmid#217683PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRB3 (BAI3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-856PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_V222A RAD23B
Plasmid#201580PurposeExpresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23BDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
UseTagsFLAGExpressionMammalianMutationContains mutation V222APromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_F400A RAD23B
Plasmid#201581PurposeExpresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23BDepositorInsertUV excision repair protein RAD23 homolog B (RAD23B Human)
UseTagsFLAGExpressionMammalianMutationContains mutation F400APromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_aa 1-420 POLI
Plasmid#201582PurposeExpresses the catalytic domain (aa 1-420) of human DNA polymerase iota with a HA tag in mammalian cellsDepositorInsertDNA polymerase iota (POLI Human)
UseTagsHAExpressionMammalianMutationExpresses amino acids 1-420 of DNA polymerase iotaPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_aa 1-449 POLH
Plasmid#201583PurposeExpresses the catalytic domain (aa 1-449) of human DNA polymerase eta with a HA tag in mammalian cellsDepositorInsertPOLH (POLH Human)
UseTagsHAExpressionMammalianMutationExpresses amino acids 1-449 of DNA polymerase etaPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_aa 1-542 POLK
Plasmid#201585PurposeExpresses the N-terminal clasp domain and catalytic domain (aa 1-542) of human DNA polymerase kappa with a HA tag in mammalian cellsDepositorInsertPolymerase kappa (POLK Human)
UseTagsHAExpressionMammalianMutationExpresses amino acids 1-542 of DNA polymerase kap…PromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_WT POLI
Plasmid#201440PurposeExpresses human DNA polymerase iota with an N-terminal HA tag in mammalian cellsDepositorInsertDNA polymerase iota (POLI Human)
UseTagsHAExpressionMammalianMutationCoding sequence has been optimised for expression…PromoterCMVAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJM919_WT His POLI
Plasmid#201444PurposeLow copy vector for expressing human DNA polymerase iota in E. coli with an N-terminal 6x His tagDepositorInsertDNA polymerase iota (POLI Human)
UseTags6x HisExpressionBacterialMutationCoding sequence has been optimised for expression…PromoterlacIqAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 HCoV-NL63 PR3_stop
Plasmid#168683PurposeGateway-compatible Entry vectorDepositorInsertPR3 (HCNV63gp3 )
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
kiCAP-AAV-PAL1A
Plasmid#196683PurposeRep/Cap plasmid for the production of PAL1A, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationPTQGTVR insert between amino acids 588 and 589 of…Promoterp41Available sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmGW182-dsRNAres_L
Plasmid#146831PurposeInsect Expression of DmGW182-dsRNAresDepositorInsertDmGW182-dsRNAres (gw Fly)
UseTagsExpressionInsectMutationTwo non silent mutations V704A and N799S and one …PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP–acVHH (Y104F)–PB
Plasmid#169683PurposeA COSMO mutant (Y104F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.DepositorInsertanti-caffeine nanobody variant
UseTagsmodified STIM1–PB tagExpressionMammalianMutationY104FPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-MIOX only
Plasmid#166683PurposeYeast integrative plasmid for expressing mouse myo-inositol oxygenase fused to GFP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertGFP-MIOX
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL10Available sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 Ub-NLRP1 S1213 P1214R
Plasmid#166830PurposepDONR221 encoding ubiquitin-tagged NLRP1-CT to generate the native S1213 N-terminus, with the P1214R patient mutation (Grandemange et al., 2017) (no stop)DepositorInsertNLRP1 (NLRP1 Human)
UseGateway shuttling vectorTagsUbiquitinExpressionMutationP1214RPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only
