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pNH-TrxT-S15

Plasmid

#127822

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pSAH0016

Plasmid

#79824

Purpose

Dual selection plasmid with lac operator, selection for binding with spectinomycin, for non-binding with chloramphenicol

Depositor

Katja Arndt

Article

Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

Inserts

cat
aadA

Promoter

AATTCAAAAAAATGCTTGACAATTAATCATCGGCTCGTATAATGTGTGG…

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-S15

Plasmid

#127823

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-Hook3 aa 1-160

Plasmid

#74607

Purpose

Expresses Hook domain of Hook3 in bacteria for purification

Depositor

Ron Vale

Article

Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

Insert

human Hook3 amino acids 1-160 (HOOK3 Human)

Tags

6x His, Strep II, and superfolder GFP

Expression

Bacterial

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pTW088

Plasmid

#115936

Purpose

3'UTR insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 3'UTR No E1

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pTW094

Plasmid

#115937

Purpose

3'UTR insert for replicon MoClo

Depositor

Ron Weiss

Article

Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

Insert

Lvl0 E1-3'UTR End

Use

Synthetic Biology

Available Since

Dec. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe hygro NFS1 CD

Plasmid

#102978

Purpose

Expression of NFS1 C381A mutant

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

NFS1 C381A (NFS1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

C381A catalytic site mutant, Silent mutations to …

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only
3563 pSFFV-neo Bad

Plasmid

#8785

Depositor

Stanley Korsmeyer

Article

Zha et al Cell 1996 Nov 15;87(4):619-28.

Insert

Bad (Bad Mouse)

Expression

Mammalian

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-FLAG-GFP-Ubl4A-C(90-157)

Plasmid

#86923

Purpose

To express Ubl4A (90-157 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 1-89 are deleted

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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spa-GCaMP6m

Plasmid

#67557

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6m

Tags

HIS-tag

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only