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pCSIIpuro-EGFP-ZO-1ΔPDZ2

Plasmid

#259704

Purpose

For introducing EGFP-ZO-1ΔPDZ2 into the genome using lentivirus.

Depositor

Kazuhiro Aoki

Publication

Hirano et al Nat Commun. 2026 May 9. doi: 10.1038/s41

Insert

ZO-1 (TJP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Lacks the PDZ2 domain

Promoter

hEF1a promoter

Available since

Sept. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-EGFP-ZO-1ΔPDZ3

Plasmid

#259705

Purpose

For introducing EGFP-ZO-1ΔPDZ3 into the genome using lentivirus.

Depositor

Kazuhiro Aoki

Publication

Hirano et al Nat Commun. 2026 May 9. doi: 10.1038/s41

Insert

ZO-1 (TJP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Lacks the PDZ3 domain

Promoter

hEF1a promoter

Available since

Sept. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-EGFP-ZO1ΔGUK

Plasmid

#259706

Purpose

For introducing EGFP-ZO-1ΔGUK into the genome using lentivirus.

Depositor

Kazuhiro Aoki

Publication

Hirano et al Nat Commun. 2026 May 9. doi: 10.1038/s41

Insert

ZO-1 (TJP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Lacks the GUK domain

Promoter

hEF1a promoter

Available since

Sept. 28, 2026

Availability

Academic Institutions and Nonprofits only

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BPK1520-sgRNA GLB1

Plasmid

#184378

Purpose

Expresses a sgRNA to edit GLB1 gene NM_000404.2_c.907A>G

Depositor

David Gilot

Publication

Leclerc et al CRISPR J. 2023 Feb;6(1):17-31. doi: 10.1

Insert

sgRNA targeting GLB1 (GLB1 Human)

Use

CRISPR

Expression

Mammalian

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pBoBi-hSEC61B-C-PEN2

Plasmid

#183648

Purpose

Lentiviral expression of a ER-residential PEN2.

Depositor

Sheng-cai Lin

Publication

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 and SEC61B (PSENEN, SEC61B Human)

Use

Lentiviral

Tags

HA

Mutation

WT

Promoter

CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pBoBi-hPEN2 2A

Plasmid

#183640

Purpose

Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the N-terminus (cytosolic face).

Depositor

Sheng-cai Lin

Publication

Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

Insert

PEN2 (PSENEN Human)

Use

Lentiviral

Tags

HA

Mutation

residues 35 and 40 of PEN2 mutated to alanine

Promoter

CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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Negative feedback (NF) plasmid

Plasmid

#169747

Purpose

Standalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.

Depositor

Francois St-Pierre

Publication

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

tetR-P2A-eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV-tetO2

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt2(K14A/R25E)-Halo-PS

Plasmid

#86632

Purpose

Expresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A/R25E)-Halo-PS (AKT2 )

Expression

Mammalian

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pOGGFPR

Plasmid

#64398

Purpose

Oat globulin Glo1 promoter driving expression of GFP

Depositor

Claudia Vickers

Publication

Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

Insert

Oat globulin Glo1 promoter

Tags

GFP

Expression

Plant

Promoter

Oat globulin Glo1

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only
pCSIIpuro-EGFP-ZO1_26E

Plasmid

#259712

Purpose

For introducing EGFP-ZO1_26E into the genome using lentivirus.

Depositor

Kazuhiro Aoki

Publication

Hirano et al Nat Commun. 2026 May 9. doi: 10.1038/s41

Insert

ZO-1 (TJP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

All S or T residues in SP or TP motifs are substi…

Promoter

hEF1a promoter

Available since

Sept. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-EGFP-ZO-1Δphos

Plasmid

#259696

Purpose

For introducing EGFP-ZO-1Δphos into the genome using lentivirus.

Depositor

Kazuhiro Aoki

Publication

Hirano et al Nat Commun. 2026 May 9. doi: 10.1038/s41

Insert

ZO-1 (TJP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

All S or T residues in SP or TP motifs are substi…

Promoter

hEF1a promoter

Available since

Sept. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-mScarlet-I-ZO-1ΔABR

Plasmid

#259698

Purpose

For introducing mScarlet-I-ZO-1ΔABR into the genome using the Tol2 transposon system.

Depositor

Kazuhiro Aoki

Publication

Hirano et al Nat Commun. 2026 May 9. doi: 10.1038/s41

Insert

ZO-1 (TJP1 Human)

Tags

mScarlet-I

Expression

Mammalian

Mutation

Lacks the actin-binding region

Promoter

CAG promoter

Available since

Sept. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pT2Apuro-mScarlet-I-ZO-1Δphos

Plasmid

#259699

Purpose

For introducing mScarlet-I-ZO-1Δphos into the genome using the Tol2 transposon system.

Depositor

Kazuhiro Aoki

Publication

Hirano et al Nat Commun. 2026 May 9. doi: 10.1038/s41

Insert

ZO-1 (TJP1 Human)

Tags

mScarlet-I

Expression

Mammalian

Mutation

All S or T residues in SP or TP motifs are substi…

Promoter

CAG promoter

Available since

Sept. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCSIIhyg-Tks5-miRFP670

Plasmid

#259701

Purpose

For introducing Tks5-miRFP670 into the genome using lentivirus.

Depositor

Kazuhiro Aoki

Publication

Hirano et al Nat Commun. 2026 May 9. doi: 10.1038/s41

Insert

Tks5 (SH3PXD2A Canis lupus familiaris (dog))

Tags

miRFP670

Expression

Mammalian

Promoter

hEF1a promoter

Available since

Sept. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-EGFP-ZO-1ΔABR

Plasmid

#259695

Purpose

For introducing EGFP-ZO-1ΔABR into the genome using lentivirus.

Depositor

Kazuhiro Aoki

Publication

Hirano et al Nat Commun. 2026 May 9. doi: 10.1038/s41

Insert

ZO-1 (TJP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Lacks the actin-binding region

Promoter

hEF1a promoter

Available since

Sept. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pT.cruzi-polyubiquitin_6xHis

Plasmid

#256080

Purpose

Production of a T. cruzi antigen, which can be used in serological assays

Depositor

Rick Tarleton

Publication

Bustamante et al PLoS Negl Trop Dis. 2022 Oct 31;16(10):e

Insert

Polyubiquitin

Tags

6xHis-STag

Expression

Bacterial

Promoter

T7lac

Available since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-Lyzc-mCherry-Rab4 DN

Plasmid

#254774

Purpose

Neutrophil specific Dominant Negative Rab4 in tol2 backbone

Depositor

Qing Deng

Publication

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Rab4 (RAB4A Human)

Use

Zebrafish expression

Mutation

DN(S27N)

Promoter

Lyzc

Available since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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Tol2-LyzC-FL Rabep1-T2A-RFP-Caax

Plasmid

#254770

Purpose

Neutrophil specific Full length Rabep1 in tol2 backbone

Depositor

Qing Deng

Publication

Kim et al J Leukoc Biol. 2026 Feb 9;118(2):qiaf174

Insert

Rabep1 (RABEP1 Human)

Use

Zebrafish expression

Promoter

Lyzc

Available since

May 4, 2026

Availability

Academic Institutions and Nonprofits only

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WoFabI-pNIC-Bsa4

Plasmid

#240437

Purpose

Expresses enoyl-ACP reductase FabI [Wolbachia endosymbiont of Brugia malayi] under T7 with HIS tag in bacterial cells)

Depositor

Josh Beckham

Insert

Enoyl-acyl carrier protein reductase

Tags

6x His-tag

Expression

Bacterial

Promoter

T7

Available since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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Dendra-Rab11a

Plasmid

#184038

Purpose

Expresses red to green photoconvertible Rab11a

Depositor

Heidi Hehnly

Publication

Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

Insert

Rab11 (RAB11A Human)

Tags

Dendra

Expression

Mammalian

Promoter

CMV

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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