We narrowed to 118,874 results for: MPI;

1. pPMS-2016

   Plasmid

   #232290

   Purpose

   Protein Expression

   Depositor

   Jeremy Nathans

   Insert

   Apcdd1 (Apcdd1 Mouse)
   

   Tags

   8X His-Avi-1D4

   Expression

   Mammalian

   Available Since

   Feb. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-N-TEV-Twin-Strep

   Plasmid

   #222014

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in E.coli

   Depositor

   Chiaki Murakami

   Type

   Empty backbone

   Tags

   TEV cleavable site (ENLYFQG) and Twin-strep-tag

   Expression

   Bacterial

   Promoter

   lac promoter

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSpec5'

   Plasmid

   #228549

   Purpose

   Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec3', to clone gRNA plasmid

   Depositor

   Jeferson Gross

   Insert

   Truncated 5' region of aadA
   

   Use

   CRISPR

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpec3'

   Plasmid

   #228548

   Purpose

   Template for PCR amplification for gRNA cloning plasmid. The PCR product recombines in vivo with a PCR product from pSpec5', to clone gRNA plasmid

   Depositor

   Jeferson Gross

   Insert

   Truncated 3' region of aadA
   

   Use

   CRISPR

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV_EF1a_mNeon

   Plasmid

   #212640

   Purpose

   Lentivirus vector to express mNeon

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   mNeon
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-EGFP-SKL

   Plasmid

   #183727

   Purpose

   For exrpression of GFP targeted to peroxisomes

   Depositor

   Jeffrey Gerst

   Article

   Haimovich et al bioRxiv 2024.11.06.622258

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   SKL (peroxisomal targeting sequence 1)

   Promoter

   UbiC

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUSE-URA3-Sec7-mEYFP

   Plasmid

   #218968

   Purpose

   Gene replacement plasmid to label S. cerevisiae Sec7 with mEYFP

   Depositor

   Benjamin Glick

   Insert

   SEC7-mEYFP (SEC7 Budding Yeast)
   

   Use

   Gene replacement

   Tags

   mEYFP

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUSE-URA-Sec7-mGold

   Plasmid

   #218967

   Purpose

   Gene replacement plasmid to label S. cerevisiae Sec7 with mGold

   Depositor

   Benjamin Glick

   Insert

   SEC7-mGold (SEC7 Budding Yeast)
   

   Use

   Gene replacement vector for yeast

   Tags

   mGold

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. YIplac211-Kar2-moxGFP2-HDEL

   Plasmid

   #218970

   Purpose

   Gene replacement plasmid to label S. cerevisiae Kar2 with moxGFP2

   Depositor

   Benjamin Glick

   Insert

   KAR2-moxGFP2-HDEL (KAR2 Budding Yeast)
   

   Use

   Gene replacement vector for yeast

   Tags

   moxGFP2

   Available Since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-IRES-tagBFP

    Plasmid

    #219965

    Purpose

    express TagBFP in mammalian cells

    Depositor

    Mohamed Fareh

    Insert

    TagBFP
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-35aaNSP1noMSA11-NSP5(io)

    Plasmid

    #220827

    Purpose

    Express the short Rotavirus SA11 NSP1 protein (35 amino acids) that lacks all methionines fused with the full-length recoded NSP5 protein

    Depositor

    Alex Borodavka

    Insert

    Rotavirus SA11 35aaNSP1noM-NSP5(io)
    

    Use

    Other

    Mutation

    the 35aa NSP1 lacks all Methionines

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. lentiGuideSLC35C1-neo

    Plasmid

    #220868

    Purpose

    encodes CRISPR sgRNA targeting human SLC3C1

    Depositor

    Jennifer Kohler

    Insert

    SLC35C1 (SLC35C1 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pT7-35aaNSP1SA11-P2A-NSP5(io)-GS-smURFP

    Plasmid

    #220828

    Purpose

    Express separately the short Rotavirus SA11 NSP1 protein (35 amino acids) and the full-length recoded NSP5 protein fused with GS-linker and smURFP

    Depositor

    Alex Borodavka

    Insert

    Rotavirus SA11 35aaNSP1-P2A-NSP5(io)-GS-smURFP
    

    Use

    Other

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-35aaNSP1noMSA11-NSP5(wt)

    Plasmid

    #220826

    Purpose

    Express the short Rotavirus SA11 NSP1 protein (35 amino acids) that lacks all methionines fused with the full-length SA11 NSP5 protein

    Depositor

    Alex Borodavka

    Insert

    Rotavirus SA11 35aaNSP1noM-NSP5(wt)
    

    Use

    Other

    Mutation

    the 35aa NSP1 lacks all Methionines

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT7-35aaNSP1SA11-Green Lantern(wt)

    Plasmid

    #220821

    Purpose

    Express the hybrid protein combining 35 amino acids from Rotavirus SA11 NSP1 protein with the Green Lantern wt protein carrying an ER-localization signal at the N- and C-termini

    Depositor

    Alex Borodavka

    Insert

    Rotavirus SA11 35aaNSP1-Green Latern(wt)
    

    Use

    Other

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-35aaNSP1SA11-Green Lantern(io)

    Plasmid

    #220822

    Purpose

    Express the hybrid protein combining 35 amino acids from Rotavirus SA11 NSP1 protein with the recoded Green Lantern protein carrying an ER-localization signal at the N- and C-termini

    Depositor

    Alex Borodavka

    Insert

    Rotavirus SA11 35aaNSP1-Green Latern(io)
    

    Use

    Other

    Available Since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. hSyn-pDisplay-SNAP[C145A]-T2A-EGFP

    Plasmid

    #223504

    Purpose

    Co-expression of dead-mutant SNAP-tags to outer surface in mammalian cells coupled with an EGFP transfection marker

    Depositor

    James Frank

    Insert

    dead-mutant SNAP-tag and EGFP
    

    Expression

    Mammalian

    Mutation

    SNAP-tag C145A

    Promoter

    hSyn

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. icLCT

    Plasmid

    #223179

    Purpose

    Native rat lactase gene (truncated) as an autologous marker (X-Gal, X-Fuc)

    Depositor

    Andreas Stuermer

    Insert

    cytosolic intestinal lactase protein fragment (Lct Rat)
    

    Expression

    Mammalian

    Available Since

    Aug. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pFastBac HTB His-TEV-rDPP9(S729A)-FLAG

    Plasmid

    #217329

    Purpose

    Purification of catalytically-dead rat DPP9 fusion with C-terminal FLAG tagfrom insect cells

    Depositor

    Yifan Wang

    Insert

    DPP9
    

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Mutation

    S729A

    Promoter

    polyhedrin

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV-GFAPp-PiGM-Iq(eGFP)

    Plasmid

    #221606

    Purpose

    A recombinant AAV2 plasmid encoding the PiGM-Iq system with CRY2PHR, CIBN and EGFP as expression marker. GFAP promoter for astrocyte-selective expression.

    Depositor

    John Lin

    Insert

    PiGM-Iq (eGFP)
    

    Use

    AAV

    Mutation

    RGS2 1-53 truncation

    Promoter

    GFAP promoter

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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