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pET28 acid.3

Plasmid

#191897

Purpose

Encodes a GFP design, acid.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

acid.3

Tags

His Tag

Expression

Bacterial

Mutation

T65S Q69L S72A T108V Y145M V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 slow.2

Plasmid

#191894

Purpose

Encodes a GFP design, slow.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

slow.2

Tags

His Tag

Expression

Bacterial

Mutation

V68M Q69L S72T V112I Y145M T167V L220V V224I

Promoter

T7

Available Since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 sf:mid.10

Plasmid

#191934

Purpose

Encodes a GFP design, sf:mid.10

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:mid.10

Tags

His Tag

Expression

Bacterial

Mutation

L42V Q69L S72A Y145F T167I L220V V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 bleach.1

Plasmid

#191885

Purpose

Encodes a GFP design, bleach.1

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.1

Tags

His Tag

Expression

Bacterial

Mutation

T108L Y145M T167V L220V

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 mid.3

Plasmid

#191884

Purpose

Encodes a GFP design, mid.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.3

Tags

His Tag

Expression

Bacterial

Mutation

S72A T108V Y145M T167V H181I V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 thermo.9

Plasmid

#191882

Purpose

Encodes a GFP design, thermo.9

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

thermo.9

Tags

His Tag

Expression

Bacterial

Mutation

T167V T203H E222Q

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 basic.3

Plasmid

#191881

Purpose

Encodes a GFP design, basic.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

basic.3

Tags

His Tag

Expression

Bacterial

Mutation

T65S V68A S72A Y145S L220V V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 mid.2

Plasmid

#191880

Purpose

Encodes a GFP design, mid.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

mid.2

Tags

His Tag

Expression

Bacterial

Mutation

T108I Y145I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 bleach.6

Plasmid

#191879

Purpose

Encodes a GFP design, bleach.6

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

bleach.6

Tags

His Tag

Expression

Bacterial

Mutation

V61L S72C Y145M H181L V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 slow.3

Plasmid

#191877

Purpose

Encodes a GFP design, slow.3

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

slow.3

Tags

His Tag

Expression

Bacterial

Mutation

Q69L S72V Y145S T167V L220V V224I

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET28 basic.2

Plasmid

#191911

Purpose

Encodes a GFP design, basic.2

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

basic.2

Tags

His Tag

Expression

Bacterial

Mutation

S72A V112I Y145V L220V V224I

Promoter

T7

Available Since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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p7142 pHAGE-P-CMVt-N-HA-GAW-ZFYV9

Plasmid

#100162

Purpose

Expresses ZFYV9

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

ZFYV9 (ZFYVE9 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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KHBD00503

Plasmid

#39606

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG6686 (CG6686 Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pHCMM1

Plasmid

#167452

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

TEF1

Availability

Academic Institutions and Nonprofits only

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pHCMM2

Plasmid

#167453

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Availability

Academic Institutions and Nonprofits only

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pHCMM3

Plasmid

#167454

Purpose

A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Article

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Availability

Academic Institutions and Nonprofits only

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Human SLC KO Library

Pooled Library

#132552

Purpose

Knockout library targeting human SLC proteins.

Depositor

Giulio Superti-Furga

Article

Girardi et al Nat Chem Biol. 2020 Apr;16(4):469-478. d

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only
pcDNA3.3-NL63-D14

Plasmid

#172666

Purpose

encodes NL63 spike protein, for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

HCNV63gp2 (HCNV63gp2 human coronavirus NL63)

Use

Lentiviral and Retroviral

Tags

NA

Expression

Mammalian

Mutation

c-terminal deletion of 14aa

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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VEE-mScarlet3-IRES-EGFP-IRES-E3

Plasmid

#242408

Purpose

Self-amplifying RNA (saRNA) E3 construct; expresses the vaccinia virus E3 protein, which binds and sequesters dsRNA broadly inhibiting dsRNA-sensing pathways

Depositor

Ewan St. John Smith

Article

Lim et al bioRxiv 2024.09.24.614636

Inserts

mScarlet3
IRES-EGFP
IRES-E3

Use

Self-amplifying rna

Expression

Mammalian

Available Since

Sept. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pKeima-LiveDrop

Plasmid

#199695

Purpose

lipophagy reporter system based on the Keima-fluorophore

Depositor

Tobias Walther

Article

Chung et al Nat Cell Biol. 2023 Aug;25(8):1101-1110.

Insert

GPAT4 (Gpat4 Fly)

Tags

Keima

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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