We narrowed to 16,604 results for: LEA
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Plasmid#136367PurposeExpresses epitope-tagged human TET3 R752C variant in mammalian cellsDepositorAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only
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pINTO-N3::hTET3 F1072C
Plasmid#136366PurposeExpresses epitope-tagged human TET3 F1072C variant in mammalian cellsDepositorAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLY57
Plasmid#130926PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-LEA5B5, and PtetAvailable SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY56
Plasmid#130925PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-LEA4B4, and PtetAvailable SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY55
Plasmid#130924PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-LEA3B3, and PtetAvailable SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY53
Plasmid#130911PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-LEA1B1, and PtetAvailable SinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
mTet1 flox donor
Plasmid#117147PurposeMaking Tet1 conditional knock outDepositorArticleInsertTet1 (Tet1 Mouse)
UseMouse TargetingAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
mTet3 flox donor
Plasmid#117146PurposeMaking Tet3 conditional knock outDepositorArticleInsertTet3 (Tet3 Mouse)
UseMouse TargetingAvailable SinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hPC1_MTS_L4139P-eGFP-3HA
Plasmid#108404PurposeExpress putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.DepositorAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mPC1_4119-eGFP
Plasmid#108394PurposeGenomic region, including intron 45, to express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293DepositorInsertPKD1 (PKD1 Mouse)
TagseGFPExpressionMammalianMutationvariant ID rs13468401 missense variant R4237QPromoterCMVAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-chimeric-IgM-mouse/human_M18
Plasmid#91738PurposeExpression plasmid coding for modified heavy chain of mouse IgM antibody. Residues 203-239 (EU numbering) were exchanged to human homologue sequence.DepositorInsertimmunoglobulin heavy constant mu (Ighm Mouse)
ExpressionMammalianMutationResidues 203-239 (EU numbering) were exchanged to…PromoterhEF1-HTLV promAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mM-Asp212Ser_M18
Plasmid#91739PurposeExpression plasmid coding for mutated heavy chain of mouse IgM antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Asp212Ser (EU numbering) - N-glycosylated Asn209DepositorInsertimmunoglobulin heavy constant mu (Ighm Mouse)
ExpressionMammalianMutationAsp212 changed to Ser (EU numbering)PromoterhEF1-HTLV promAvailable SinceJune 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMSCV40-EF1a-hBCL10(1-114)-SO
Plasmid#75257Purposeretroviral expression vector for expression of StrepOne-Tagged human BCL10 (AS 1-116)DepositorInsertBcl10 (BCL10 Human)
UseRetroviralTagsStrepOne TagExpressionMammalianMutationAS 1-116 (CARD-domain)PromoterpMSCV-LTRs, EF1aAvailable SinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cer(159-238)-beta-5 in pcDNAI/Amp
Plasmid#55778PurposeA carboxyl-terminal mCerulean fragment was fused to Gbeta-5. When co-expressed with an amino terminal mCerulean fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertCer(159-238)-beta-5 (GNB5 Human, Aequorea victoria)
TagsmCer(159-238) was fused to the amino terminus of …ExpressionMammalianMutationGBeta-5 was amplified via PCR, which added an N-t…PromoterCMVAvailable SinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST26 Ago2 4A
Plasmid#59907PurposeExpresses 6xHis-Ago2 (N-termi tag) mutated four serines to alanines in mammalian cellsDepositorInsertArgonaute 2 (AGO2 Human)
Tags6xHis (N-termi tag)ExpressionMammalianMutationmutated four serines to alaninesPromoterCMVAvailable SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
im:7163548_L (OZ571)
Plasmid#35211DepositorInsertZinc finger array targeting im:7163548 (im:7163548 Zebrafish)
UseZebrafish targetingAvailable SinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
zgc:162148_L (OZ579)
Plasmid#35219DepositorInsertZinc finger array targeting zgc:162148 (micu1 Zebrafish)
UseZebrafish targetingAvailable SinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
im:7163548_R (OZ572)
Plasmid#35212DepositorInsertZinc finger array targeting im:7163548 (im:7163548 Zebrafish)
UseZebrafish targetingAvailable SinceMarch 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-OSK
Plasmid#136552PurposeDox-inducible polycistronic reprogramming lentiviral vector expressing mouse Oct4, Sox2, Klf4DepositorUseLentiviralExpressionBacterialPromotertetO-miniCMV (dox-inducible)Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Lamin-A
Plasmid#206027PurposeExpresses GFP-tagged Lamin A in mammalian cellsDepositorAvailable SinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only