We narrowed to 16,278 results for: nes
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Plasmid#121667PurposeEFS-Cas9-wt-P2A-PuroDepositorInsertcas9
UseLentiviralPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBID2_UAS>MCS
Plasmid#194958PurposeDrosophila transgene expression under UAS/Gal4 control, PhiC31 transgenesisDepositorPublicationTypeEmpty backboneExpressionInsectMutation20 x UAS sequencesAvailable sinceOct. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBID2_MCS>Gal4
Plasmid#194961PurposeDrosophila Gal4 expression under introduced sequence control, PhiC31 transgenesisDepositorPublicationTypeEmpty backboneExpressionInsectMutationGal4.2Available sinceOct. 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
p5E-CAGGS (JDW 912)
Plasmid#224472PurposeGateway compatible 5' entry clone containing the CAG promoter for constitutive expression of downstream constructs.DepositorInsertCAGGS Promoter
ExpressionMammalianAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
ExpressionBacterialPromoterT7 promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pMBA323
Plasmid#214751PurposeTo create delta(glmS,nadE) working strains and maintaining their viabilityDepositorInsertBBa_J23105-cas9 + pBAD-λ Red genes + ptet-gRNA (pBR322ori)
UseCRISPRExpressionBacterialAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
IDG_KCNJ14_OE_1
Plasmid#161691PurposeOverexpresses KCNJ14DepositorAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICSL1P1_crU626
Plasmid#191776PurposeCas12a guide expression in dicotsDepositorInsertLbCas12a guide scaffold with AtU626 promoter/terminator & flanking ribozymes
UseCRISPRExpressionPlantAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-11
Plasmid#160711PurposeFor C-terminal tags with Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sitesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTrichoderma optimised Synthetic eGFP
UseSynthetic BiologyAvailable sinceMarch 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD421
Plasmid#163112PurposeProduction of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysSDepositorInsertHis-tag_TEV-cleavable-linker_mEos3.2_H-NSdbd
TagsHis-tag with TEV-cleavable linkerExpressionBacterialPromoterT7 promoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 TIM8,13
Plasmid#170282PurposeExpresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli productionDepositorInsertyeast Tim8 and Tim13 (TIM8 Budding Yeast)
TagsTEV-cleavable N-terminal His tag on Tim13 (on bac…ExpressionBacterialAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgNeg2
Plasmid#166999PurposeLentiviral expression of negative control sgRNA for CRISPRi knockdownDepositorInsertNegative control sgRNA 2
UseCRISPR and LentiviralPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL1-TTlight-4xDQNAT
Plasmid#128394PurposeIn vitro expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sitesDepositorInsertTetanus toxin light chain domain
Tags4xDQNAT glycosylation tag and 6xHis tagExpressionBacterialMutationInactivating E234A mutationPromoterT7Available sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2214 - Intron GG1 - 900 bp
Plasmid#159880PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG1 - 900 bp
ExpressionWormMutationNot applicableAvailable sinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-10
Plasmid#160710PurposeFor C-terminal tags with Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sitesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTrichoderma optimised Synthetic mCherry
UseSynthetic BiologyAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-8
Plasmid#160708PurposeFor N-terminal tags with Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sitesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTrichoderma optimised Synthetic eGFP
UseSynthetic BiologyAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-7
Plasmid#160707PurposeFor N-terminal tags with Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sitesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTrichoderma optimised Synthetic mCherry
UseSynthetic BiologyAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1359 - Intron GG2 - smu-1
Plasmid#159805PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertIntron GG2 - smu-1
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0384
Plasmid#133234PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable sinceApril 8, 2020AvailabilityAcademic Institutions and Nonprofits only