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YIp211-P30-His-pol30(K127/164R)-3'

Plasmid

#99549

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K127R, K164R

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF_6His-ZmSUMO1a-GG_ZmSCE1b-Myc

Plasmid

#91932

Purpose

Bacterial expression of maize SUMO1a and SCE1b

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

6His-ZmSUMO1a-GG
ZmSCE1b-Myc

Tags

6His and Myc

Expression

Bacterial

Mutation

Truncation of ZmSUMO1a after the diGlycine motif

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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PFKFB4 gRNA (BRDN0001149495)

Plasmid

#77123

Purpose

3rd generation lentiviral gRNA plasmid targeting human PFKFB4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PFKFB4 (PFKFB4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

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PIP5K1A gRNA (BRDN0001147420)

Plasmid

#77964

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIP5K1A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIP5K1A (PIP5K1A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PAK4 gRNA (BRDN0001148325)

Plasmid

#77576

Purpose

3rd generation lentiviral gRNA plasmid targeting human PAK4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PAK4 (PAK4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CRKL gRNA (BRDN0001145388)

Plasmid

#77508

Purpose

3rd generation lentiviral gRNA plasmid targeting human CRKL

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CRKL (CRKL Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CRKL gRNA (BRDN0001148341)

Plasmid

#77509

Purpose

3rd generation lentiviral gRNA plasmid targeting human CRKL

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CRKL (CRKL Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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GSK3A gRNA (BRDN0001148594)

Plasmid

#76370

Purpose

3rd generation lentiviral gRNA plasmid targeting human GSK3A

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

GSK3A (GSK3A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 TCF3

Plasmid

#67110

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCF3 (TCF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

Truncated isoform

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-TODestFLAGHA_RBPMS2

Plasmid

#59390

Purpose

Destination vector with RBPMS2 for stable cell line generation

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS2 (RBPMS2 Human)

Tags

FLAG HA and V5

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Feb. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pRKVSV FRNK

Plasmid

#50534

Depositor

Kenneth Yamada

Article

Segarra et al FASEB J. 2005 Nov;19(13):1875-7.

Insert

protein tyrosine kinase, domain

Tags

2X VSV

Expression

Mammalian

Mutation

truncated (NT2186-3268)

Promoter

CMV

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-Xl-VSP1 C301S

Plasmid

#51882

Purpose

expression of Xl-VSP1 inactive mutant in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP1 C301S (tpte2 Frog)

Use

Xenopus oocyte expression

Expression

Mammalian

Mutation

C301S, catalytically-inactive mutant of Xl-VSP1

Promoter

CMV

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pET15b-At 14-3-3 omega

Plasmid

#44930

Depositor

Robert Ferl

Article

Wu et al Arch Biochem Biophys. 1997 Mar 1;339(1):

Insert

14-3-3 omega (GRF2 Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
ABL2

Plasmid

#39178

Purpose

Baculovirus expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

ABL2 (ABL2 Human)

Use

Baculovirus expression

Tags

His Tev

Mutation

Truncated (see comments)

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
YFP-Gamma 9-3

Plasmid

#36074

Depositor

Narasimhan Gautam

Article

Chisari et al PLoS One. 2009 Nov 11;4(11):e7797.

Insert

G protein G9(gamma 3 tail)

Tags

YFP

Expression

Mammalian

Mutation

HIs tag between YFP and insert

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
Hox D13a

Plasmid

#36467

Depositor

Robert Ho

Article

Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

Insert

hoxa11b (hoxa11b Zebrafish)

Use

Riboprobe synthesis

Mutation

F63I, P75Q and M133I

Promoter

T7

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only
M48 GST-TEV-Chibby-N

Plasmid

#17196

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

Chibby (CBY1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-63

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
EC.RNe.TYB1.a1-400.wt

Plasmid

#17949

Depositor

David McKay

Article

Caruthers et al J Biol Chem. 2006 Sep 15. 281(37):27046-

Insert

Escherichia coli RNase E residues 1-400

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

residues 1-400

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
p067 pCS mTRPS1 P4

Plasmid

#11039

Depositor

Ramesh Shivdasani

Article

Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Insert

TRPS1 P4 (Trps1 Mouse)

Use

Xenopus, mammalian, avian, and zebrafish

Mutation

Truncation at aa1121 to mimic frameshift at codon…

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only
pGL2_136

Plasmid

#199083

Purpose

Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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