We narrowed to 17,148 results for: tes

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pUPD2+p35SoTMV

Plasmid

#170876

Purpose

Phytobrick - p35SoTMV

Depositor

Markita Landry

Article

Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

Insert

p35SoTMV

Expression

Plant

Mutation

214C>T and 229C>T to remove BbsI restrictio…

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-GFP-SFPQY527A-IRES-mCherry

Plasmid

#166951

Purpose

Lentiviral plasmid expressing GFP-tagged SFPQ Y527A protein with IRES-mCherry from the EF1a promoter

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ-Y527A (SFPQ Human)

Use

Lentiviral

Tags

GFP and mCherry

Mutation

SFPQ-Y527A

Promoter

eF1a

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pRG659_lexO-FseI_LexA-TF_LEU2MX

Plasmid

#154816

Purpose

Inducible expression of FseI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

FseI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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EC71171

Plasmid

#154069

Purpose

Level 0 Golden Gate vector, CRE with U5 intron at 254bp, in pICH41308 backbone

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

CRE_U5intron

Use

Synthetic Biology

Mutation

All BsaI, Esp3I and BPiI restriction sites were r…

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaCTD-HA.SV40(polyA)

Plasmid

#156163

Purpose

Expresses truncated (AA 1-954) murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

Truncated THBS1 (amino acid residues 1-954)

Promoter

CMV

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaTSR1-HA.SV40(polyA)

Plasmid

#156162

Purpose

Expresses murine Thrombospondin-1 (THBS1) with deletion (AA 383-548) and HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

THBS1 with deletion of amino acid residues 383-548

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-2_dCrk

Plasmid

#131146

Purpose

Expresses GST::dCrk (based on crk isoform RA).

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

GST

Expression

Bacterial

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCIneoLuc-PP1A

Plasmid

#128348

Purpose

Vector for LUMIER assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

PP1A (PPP1CA Human)

Tags

Luciferase

Expression

Mammalian

Available Since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_sgFLNB-SK2

Plasmid

#119873

Purpose

gRNA expression vector

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgFLNB-SK2 (FLNB Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pXPR_sgFLNB-SK4

Plasmid

#119874

Purpose

gRNA expression vector

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgFLNB-SK4 (FLNB Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pF712-pET28a-Hs-delta44-GCDH-NHis wt

Plasmid

#85113

Purpose

expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Article

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human mature (delta44) GCDH (GCDH Human)

Tags

6xHis

Expression

Bacterial

Mutation

silent SNP: G1041T

Promoter

T7

Available Since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Braf R188L

Plasmid

#118330

Purpose

Expression of human Braf

Depositor

Philip Stork

Article

Li et al J Biol Chem. 2013 Sep 20;288(38):27646-5

Insert

Braf R188L (BRAF Human)

Tags

GFP

Expression

Mammalian

Mutation

R188L

Promoter

CMV

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ239-RR

Plasmid

#108862

Purpose

FnCpf1 Gateway entry plasmid with N623R and K687R mutations

Depositor

Yiping Qi

Article

Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

Insert

FnCpf1-RR (FnCpf1 with N623R and K687R mutations)

Use

CRISPR; Gateway compatible cpf1 entry clone

Tags

NLS

Expression

Plant

Mutation

N623R and K687R, Cpf1 is rice codon optimized

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING-V155M S366A

Plasmid

#102593

Purpose

Lentivector to express STING V155M S366A and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

V155M S366A

Promoter

SFFV

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pE-cadherin-GFP-N-WASP deltaVCA

Plasmid

#101283

Purpose

Mammalian expression of E-cadherin-GFP-N-WASP delta VCA

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-Cadherin-GFP-N-WASP (Cdh1, Wasl Mouse)

Tags

GFP

Expression

Mammalian

Mutation

delta VCA domain

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-mouse Gfi1-IRES-GFP

Plasmid

#91893

Purpose

Mammalian expression of mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

Gfi1 (Gfi1 Mouse)

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CAGGS

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
pGLS3-5xHRE-PHLDA3(wt)

Plasmid

#72557

Purpose

Hypoxia induced mutant PHLDA3. Contains the HRE from VEGF sequence (5 repeats) upstream of PHLDA3.

Depositor

Ester Hammond

Article

Leszczynska et al J Clin Invest. 2015 Jun;125(6):2385-98.

Insert

PHLDA3 (PHLDA3 Human)

Tags

5XHRE

Expression

Mammalian

Promoter

E1b minimal

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SLAP-FLAG

Plasmid

#74500

Purpose

Overexpression of SLAP in mammalian cells

Depositor

Lars Rönnstrand

Article

Kazi et al PLoS One. 2012;7(12):e53509. doi: 10.137

Insert

SLAP (SLA Human)

Expression

Mammalian

Mutation

N18K, G74A, and T149A

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pCDNA3 Flag MFF iso5 S172A

Plasmid

#74382

Purpose

Flag tagged human S172A MFF isoform 5 mutant

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser 172 Ala (numbering based on MFF isoform 1)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
mHDAC7-Nterm (23-504) pEF6-V5

Plasmid

#73270

Purpose

Expression of mouse HDAC7-Nterm in mammalian cells (V5 tag)

Depositor

Matthew Sweet

Article

Shakespear et al J Biol Chem. 2013 Aug 30;288(35):25362-7

Insert

HDAC7 (Hdac7 Mouse)

Tags

His/V5

Expression

Mammalian

Mutation

deleted amino acids 505-938

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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