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TadCBE-Cas9NG

Plasmid

#225093

Purpose

Plasmid for bacterial purification of codon-optimized TadCBE-Cas9NG

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Science. 2025 Apr 4;388(6742):52-59. doi

Insert

TadCBE-Cas9NG

Use

CRISPR

Tags

8xHis

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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TadCBE-Cas9NG

Plasmid

#225093

Purpose

Plasmid for bacterial purification of codon-optimized TadCBE-Cas9NG

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Science. 2025 Apr 4;388(6742):52-59. doi

Insert

TadCBE-Cas9NG

Use

CRISPR

Tags

8xHis

Expression

Bacterial

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pOxyR_gfp

Plasmid

#194154

Purpose

Green Fluorescent Reporter of intracellular hydrogen peroxide

Depositor

Sven Panke

Article

Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

Insert

transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP

Expression

Bacterial

Promoter

PoxyR, PoxyS

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pOxyR_gfp

Plasmid

#194154

Purpose

Green Fluorescent Reporter of intracellular hydrogen peroxide

Depositor

Sven Panke

Article

Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

Insert

transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein sfGFP

Expression

Bacterial

Promoter

PoxyR, PoxyS

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEmpty_Control

Plasmid

#162607

Purpose

Empty control for Cas9 experiments and to clone new guide RNAs for paper

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pEmpty_Control

Plasmid

#162607

Purpose

Empty control for Cas9 experiments and to clone new guide RNAs for paper

Depositor

Lars Steinmetz

Article

Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

Insert

Tef1-Cas9 with RPL25 Intron

Expression

Yeast

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBa.Kif1a 1-396.GFP

Plasmid

#45058

Depositor

Gary Banker, Marvin Bentley

Article

Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

Insert

Kif 1a (Kif1a Rat)

Tags

GFP

Expression

Mammalian

Mutation

motor domain aa 1-396

Promoter

Chicken Beta Actin

Available Since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only
pBa.Kif1a 1-396.GFP

Plasmid

#45058

Depositor

Gary Banker, Marvin Bentley

Article

Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

Insert

Kif 1a (Kif1a Rat)

Tags

GFP

Expression

Mammalian

Mutation

motor domain aa 1-396

Promoter

Chicken Beta Actin

Available Since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only
RNAPII_CTD27-52

Plasmid

#98678

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52

Plasmid

#98678

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pOxyR_rfp

Plasmid

#194155

Purpose

Red Fluorescent Reporter of intracellular hydrogen peroxide

Depositor

Sven Panke

Article

Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

Insert

transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry

Expression

Bacterial

Promoter

PoxyR, PoxyS

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pOxyR_rfp

Plasmid

#194155

Purpose

Red Fluorescent Reporter of intracellular hydrogen peroxide

Depositor

Sven Panke

Article

Kardashliev et al Chem Sci. 2021 Oct 29;12(44):14766-14772

Insert

transcriptional regulator, OxyR, cognate promoter PoxyS, fluorescent protein mCherry

Expression

Bacterial

Promoter

PoxyR, PoxyS

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAB1

Plasmid

#62547

Purpose

broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.

Depositor

Michael Franklin

Article

Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47

Insert

eGFP

Use

Broad host range plasmid

Expression

Bacterial

Promoter

pTrc

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pAB1

Plasmid

#62547

Purpose

broad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.

Depositor

Michael Franklin

Article

Walters MC et al Antimicrob Agents Chemother. 2003 Jan;47

Insert

eGFP

Use

Broad host range plasmid

Expression

Bacterial

Promoter

pTrc

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR

Plasmid

#194063

Purpose

Mammalian expression of evoCjCas9 PEmax_deltaRnH

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-PEmax_dRnH

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR

Plasmid

#194063

Purpose

Mammalian expression of evoCjCas9 PEmax_deltaRnH

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-PEmax_dRnH

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTlpA_mCherry_YafQ/DinJ

Plasmid

#229148

Purpose

Toxin-Antitoxin (Dinj/YafQ)

Depositor

Shrikrishnan Sankaran

Article

Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

Insert

mCherry, DinJ/YafQ

Expression

Bacterial

Promoter

PtlpA (mCherry), DinJ/YafQ (Native)

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTlpA_mCherry_YafQ/DinJ

Plasmid

#229148

Purpose

Toxin-Antitoxin (Dinj/YafQ)

Depositor

Shrikrishnan Sankaran

Article

Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

Insert

mCherry, DinJ/YafQ

Expression

Bacterial

Promoter

PtlpA (mCherry), DinJ/YafQ (Native)

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-ABE8e-BlastR

Plasmid

#194060

Purpose

Mammalian expression of evoCjCas9 ABE8e

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-ABE8e

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-ABE8e-BlastR

Plasmid

#194060

Purpose

Mammalian expression of evoCjCas9 ABE8e

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-ABE8e

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge