Skip to main content
Addgene
Showing: 841 - 860 of 2084 results

  1. enIscB-T5E

    Plasmid
    #205411
    Purpose
    Vector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Yingsi Zhou
    Article
    Han et al Nat Methods. 2023 May 25. doi: 10.1038/s
    Insert
    pCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E85R+H369R+S387R+S457R
    Promoter
    CAG, hU6, CMV
    Available since
    Dec. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    205411-plasmid-map-sequence-id-416557

    Enlarge

  2. pPBC-G6s

    Plasmid
    #62812
    Purpose
    pPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-GCaMP6s-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    62812-plasmid-map-sequence-id-123114

    Enlarge

  3. pPBC-LG6s

    Plasmid
    #62809
    Purpose
    pPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-Lck-GCaMP6s-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    62809-plasmid-map-sequence-id-123119

    Enlarge

  4. pPBC-G6f

    Plasmid
    #62811
    Purpose
    pPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-GCaMP6f-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    62811-plasmid-map-sequence-id-123116

    Enlarge

  5. pPBC-LG6f

    Plasmid
    #62808
    Purpose
    pPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-Lck-GCaMP6f-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    62808-plasmid-map-sequence-id-123121

    Enlarge

  6. pPBC-G3-tdT

    Plasmid
    #62814
    Purpose
    pPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked GCaMP3 and tdTomato under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-GCaMP3-IRES-tdTomato-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    62814-plasmid-map-sequence-id-121226

    Enlarge

  9. pRegev_ExonSkip_Backbone

    Plasmid
    #204187
    Purpose
    Backbone for cloning splicing library with truncated CAG promoter and first half of beta globin minigene reporter (exon 1 and intron 1).
    Depositor
    Oded Regev
    Article
    Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12
    Insert
    First exon and intron for beta globin splicing minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Truncated CAG
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    204187-plasmid-map-sequence-id-404040

    Enlarge

  10. PB-FLuc+GFPd2

    Plasmid
    #127190
    Purpose
    Piggybac transposon plasmid for expression of CMV promoter firefly luciferase (FLuc) and SV40 destabilized green fluorescent protein (GFPd2)
    Depositor
    Jordan Green
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    127190-plasmid-map-sequence-id-248023

    Enlarge

  11. TRE-MCS-WPRE-SV40 - GCH25

    Plasmid
    #230826
    Purpose
    AAV cloning vector for expression under the TRE promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    230826-plasmid-map-sequence-id-459123

    Enlarge

  12. Syn-MCS-WPRE-SV40 - GCH7

    Plasmid
    #230817
    Purpose
    AAV cloning vector for expression under the Syn promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230817-plasmid-map-sequence-id-457138

    Enlarge

  13. CaMKII-MCS-WPRE-SV40 - GCH19

    Plasmid
    #230823
    Purpose
    AAV cloning vector for expression under the CaMKII promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230823-plasmid-map-sequence-id-457140

    Enlarge

  14. EF1a-MCS-WPRE-SV40 - GCH13

    Plasmid
    #230820
    Purpose
    AAV cloning vector for expression under the EF1a promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230820-plasmid-map-sequence-id-457137

    Enlarge

  15. GFAP-MCS-WPRE-SV40 - GCH31

    Plasmid
    #230829
    Purpose
    AAV cloning vector for expression under the GFAP promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV; Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230829-plasmid-map-sequence-id-457134

    Enlarge

  16. pPBC-G6s

    Plasmid
    #62812
    Purpose
    pPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-GCaMP6s-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    62812-plasmid-map-sequence-id-123114

    Enlarge

  17. pPBC-LG6s

    Plasmid
    #62809
    Purpose
    pPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-Lck-GCaMP6s-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    62809-plasmid-map-sequence-id-123119

    Enlarge

  18. pPBC-G6f

    Plasmid
    #62811
    Purpose
    pPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-GCaMP6f-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    62811-plasmid-map-sequence-id-123116

    Enlarge

  19. pPBC-LG6f

    Plasmid
    #62808
    Purpose
    pPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-Lck-GCaMP6f-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    62808-plasmid-map-sequence-id-123121

    Enlarge

  20. pPBC-G3-tdT

    Plasmid
    #62814
    Purpose
    pPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked GCaMP3 and tdTomato under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Article
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-GCaMP3-IRES-tdTomato-ITR
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CAG
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    62814-plasmid-map-sequence-id-121226

    Enlarge

Showing: 841 - 860 of 2084 results