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pdest METTL1-S27A Neo

Plasmid

#223059

Purpose

METTL1-S27A gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-S27A (METTL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S27A

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pdest METTL1-S27D Neo

Plasmid

#223060

Purpose

METTL1-S27D gene expression in mammalian cells

Depositor

Alejandro Gutierrez

Article

Ali et al Mol Cell. 2025 Mar 6;85(5):948-961.e11.

Insert

METTL1-S27D (METTL1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

S27D

Promoter

CMV

Available Since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLYS6-METTL17-LYRMut-FLAG

Plasmid

#220877

Purpose

Expressing METTL17-LYRMut-FLAG

Depositor

Vamsi Mootha

Article

Ast et al Mol Cell. 2024 Jan 18;84(2):359-374.e8.

Insert

METTL17 (METTL17 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Changed the LYP domain to AAA

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV4-HA-p31(comet)-S102A

Plasmid

#221698

Purpose

Mammalian expression of HA-tagged human p31(comet) (S102A)

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

MAD2BP1

Expression

Mammalian

Mutation

S102A

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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LMPd Ametrine Got1 shRNA#1

Plasmid

#220592

Purpose

Retroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffold

Depositor

Russell Jones

Article

Ma et al bioRxiv [Preprint]. 2023 Jun 11:2023.06.

Insert

Got1 shRNA (Got1 Mouse)

Use

Retroviral

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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P1K-3HA-Atg8∆GR-MET15

Plasmid

#207033

Purpose

Expression of 3HA-Atg8∆GR. This variant cannot be conjugated to PE.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xHA

Expression

Yeast

Mutation

∆GR

Promoter

pATG8

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO shLuc/FLAG Metap2

Plasmid

#209316

Purpose

Lentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA)

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

METAP2 (METAP2 human, Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO shLYCHOS/FLAG Metap2

Plasmid

#209314

Purpose

Lentiviral construct expressing FLAG Metap2 and shRNA against LYCHOS

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

PGK

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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MacMT3 Musa acuminata Metallothionein 3

Plasmid

#200294

Purpose

Bacterial expression plasmids for production of recombinant Musa acuminata Metallothionein 3

Depositor

Artur Krężel

Article

Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

Insert

Gene of metallothionein with Chiting Binding Domanin-Intein

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available Since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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TvMT Thermosynechococcus vulcanus Metallothionein

Plasmid

#200299

Purpose

Bacterial expression plasmids for production of recombinant Thermosynechococcus vulcanus Metallothionein

Depositor

Artur Krężel

Article

Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

Insert

Gene of metallothionein with Chiting Binding Domanin-Intein

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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XlMT Xenopus laevis Metallothionein

Plasmid

#200293

Purpose

Bacterial expression plasmids for production of recombinant Xenopus laevis Metallothionein

Depositor

Artur Krężel

Article

Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

Insert

Gene of metallothionein with Chiting Binding Domanin-Intein

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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PflQ2MT Pseudomonas fluorescens Q2-87 Metallothionein

Plasmid

#200297

Purpose

Bacterial expression plasmids for production of recombinant Pseudomonas fluorescens Q2-87 Metallothionein

Depositor

Artur Krężel

Article

Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

Insert

Gene of metallothionein with Chiting Binding Domanin-Intein

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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BcrMT1B Blue Crab Metallothionein 1B

Plasmid

#200290

Purpose

Bacterial expression plasmids for production of recombinant Blue Crab Callinectes sapidus Metallothionein 1B

Depositor

Artur Krężel

Article

Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

Insert

Gene of metallothionein with Chiting Binding Domanin-Intein

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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LlMT Littorina littorea Metallothionein

Plasmid

#200291

Purpose

Bacterial expression plasmids for production of recombinant Littorina littorea Metallothionein

Depositor

Artur Krężel

Article

Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

Insert

Gene of metallothionein with Chiting Binding Domanin-Intein

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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OsMTI-1B Oryza sativa Metallothionein 1B

Plasmid

#200295

Purpose

Bacterial expression plasmids for production of recombinant Oryza sativa Metallothionein 1B

Depositor

Artur Krężel

Article

Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

Insert

Gene of metallothionein with Chiting Binding Domanin-Intein (LOC4332487 Oryza sativa)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-mAmetrine1.1-WPRE

Plasmid

#193333

Purpose

Expresses mAmetrine1.1 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mAmetrine1.1

Expression

Mammalian

Promoter

TRE

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pX458-MMETn-del-gRNA2

Plasmid

#185007

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

MMETn-del-gRNA2

Use

CRISPR

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMETn_2

Plasmid

#185019

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMETn

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMETn_1

Plasmid

#185018

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMETn

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-MMETn-del-gRNA1

Plasmid

#185006

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

MMETn-del-gRNA1

Use

CRISPR

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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