We narrowed to 1,231 results for: POLI

1. pTU1-D-RFP

   Plasmid

   #72942

   Purpose

   Level 1 Destination vector - Position D

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 1 - D site
   

   Use

   Synthetic Biology

   Mutation

   Beta-lactamase - G717C (nucleotide) - silent muta…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTU1-D1-RFP

   Plasmid

   #72943

   Purpose

   Level 1 Destination vector - Position D1

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 1 - D1 site
   

   Use

   Synthetic Biology

   Mutation

   Beta-lactamase - G717C (nucleotide) - silent muta…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTU1-E-RFP

   Plasmid

   #72944

   Purpose

   Level 1 Destination vector - Position E

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 1 - E site
   

   Use

   Synthetic Biology

   Mutation

   Beta-lactamase - G717C (nucleotide) - silent muta…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBP-ORF-eGFP

   Plasmid

   #72960

   Purpose

   Level 0 Entry vector" ORF - eGFP "

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Enhanced green flourescent protein (eGFP)
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCellFree_G03 RRN3

   Plasmid

   #67112

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   RRN3 (RRN3 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCellFree_G03 TFAP4

   Plasmid

   #67073

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TFAP4 (TFAP4 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Mutation

   P338A

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBP-lacZ

   Plasmid

   #72948

   Purpose

   Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 0 - Bioparts
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ki67∆FHA-mNeonGreen-IRESpuro2

   Plasmid

   #183739

   Purpose

   Expresses Ki67∆FHA-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67_lacking FHA (MKI67 Human)
   

   Tags

   mNeonGreen

   Expression

   Bacterial and Mammalian

   Mutation

   MKI67(lacking FHA)

   Promoter

   CMV

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMP11

   Plasmid

   #215559

   Purpose

   pKD46 with constitutively expressed Cas9 and an aTc gRNA targeting the ColE1 origin

   Depositor

   Brian Pfleger

   Article

   Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

   Inserts

   Cas9
   gRNA targeting pBR322 origin of replication
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23105 and tet

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBP-J23100

    Plasmid

    #72963

    Purpose

    Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    J23100 promoter
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. H2B-2A-GFP-IRESpuro2

    Plasmid

    #183746

    Purpose

    Expresses H2B-2A-GFP

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    H2B (H2BC16P Human)
    

    Tags

    GFP with 2A protease cleavage site between H2B an…

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDECKO_mCherry

    Plasmid

    #78534

    Purpose

    Backbone to clone single or paired sgRNAs

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (for expressing sgRNA)

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. EGFP-Ki67-mCherry

    Plasmid

    #183748

    Purpose

    Expresses EGFP-Ki67-mCherry

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 (MKI67 Human)
    

    Tags

    Ki-67 with N-terminal EGFP tag and Ki-67 with C-…

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. EpsilonCOP EGFP

    Plasmid

    #128151

    Purpose

    Expression of epsilon COP

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

    Insert

    epsilon COP (COPE Bovine)
    

    Expression

    Mammalian

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCellFree_G03 CYP2D6

    Plasmid

    #67090

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CYP2D6 (CYP2D6 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCellFree_G03 CYP3A4

    Plasmid

    #67104

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    CYP3A4 (CYP3A4 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFAP-Cre

    Plasmid

    #24704

    Depositor

    Michael Sofroniew

    Article

    Bush et al Neuron. 1999 Jun . 23(2):297-308.

    Insert

    Cre
    

    Expression

    Mammalian

    Promoter

    GFAP (glial fibrillary acidic protein)

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ki67∆Nterm-mNeonGreen-IRESpuro2

    Plasmid

    #183740

    Purpose

    Expresses Ki67∆Nterm-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain + all Ki-67 repeats (MKI67 Human)
    

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EGFP-mCherry-Ki67

    Plasmid

    #183750

    Purpose

    Expresses EGFP-mCherry-Ki67

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 (MKI67 Human)
    

    Tags

    EGFP and mCherry

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2

    Plasmid

    #183741

    Purpose

    Expresses Ki67 (8 repeats+LR)-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
    

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    MKI67 LR domain + 8 Ki-67 repeats

    Promoter

    CMV

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge