We narrowed to 7,445 results for: ski

1. pBetaActin-FRB-dCer3-CAAX

   Plasmid

   #191326

   Purpose

   Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   FRB-dCer-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBetaActin-TUBB2a-mEos3.2

   Plasmid

   #191320

   Purpose

   Expresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   TUBB2a-mEos3.2 (Tubb2a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBetaActin-TUBB2a-Dronpa

   Plasmid

   #191321

   Purpose

   Expresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV light

   Depositor

   Frank Bradke

   Article

   Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

   Insert

   TUBB2a-Dronpa (Tubb2a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJEP229-Lenti-TRE3G-4xMyc-GluN2B-WPRE-pA

   Plasmid

   #164794

   Purpose

   pLenti vector backbone designed to express 4XMyc Tagged-GluN2B from a TRE3G promoter.

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   GluN2B
   

   Use

   Lentiviral

   Tags

   4xMyc

   Expression

   Mutation

   Promoter

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p09008_AF1-4+GFP5-7

   Plasmid

   #173715

   Purpose

   Plasmid backbone for the HyperXpress workflow containing new to nature hybrid GFP with strong fluorescence in E. coli cell free protein synthesis extracts.

   Depositor

   Johannes Kabisch

   Article

   Zibulski et al Front Bioeng Biotechnol. 2022 Apr 1;10:8

   Inserts

   hybrid GFP
   mKate2
   

   Use

   E. coli cell free protein synthesis extracts

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJEP231-Lenti-TRE3G-Flag-GluN2B(E1479Q)-WPRE-pA

   Plasmid

   #164796

   Purpose

   pLenti vector backbone designed to express Flag Tagged-GluN2B(E1479Q) from a TRE3G promoter.

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   GluN2B
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJEP201-Lenti-TRE3G-Flag-GluN2B-WPRE-pA

   Plasmid

   #164775

   Purpose

   pLenti vector backbone designed to express Flag-GluN2B from a TRE3G promoter.

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   GluN2B
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJEP226-Lenti-CMV-Flag-GluN2B-WRPE-pA

   Plasmid

   #84275

   Purpose

   pLenti vector backbone designed to express Flag-GluN2B from a CMV promoter.

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   GluN2B
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   CMV

   Available since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJEP225-Lenti-CMV-Flag-GluN2A-WPRE-pA

   Plasmid

   #84274

   Purpose

   pLenti vector backbone designed to express Flag-GluN2A from a CMV promoter.

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   Flag-GluN2A
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJEP200-Lenti-TRE3G-Flag-GluN2A-WPRE-pA

    Plasmid

    #164724

    Purpose

    pLenti vector backbone designed to express Flag-GluN2A from a TRE3G promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    GluN2A
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    March 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJEP202-Lenti-TRE3G-GFPGluN2A-WPRE-pA

    Plasmid

    #164776

    Purpose

    pLenti vector backbone designed to express GFP-GluN2A from a TRE3G promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    GluN2A
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    1.3αCaMKII

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJEP214-Lenti-0.5Synapsin P-GFP- WPRE-pA

    Plasmid

    #62929

    Purpose

    pLenti vector backbone designed to express GFP from a 0.5Synapsin promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    0.5synapsin and 1.3αCaMKII

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJEP215-Lenti-0.4CaMKIIa P-GFP-WPRE-pA

    Plasmid

    #62927

    Purpose

    pLenti vector backbone designed to express GFP from a 0.4CaMKIIa promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    0.4αCaMKII and 1.3αCaMKII

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJEP213-Lenti-1.1Synapsin P-GFP-WPRE-pA

    Plasmid

    #62930

    Purpose

    pLenti vector backbone designed to express GFP from a 1.1Synapsin promoter .

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    1.1Synapsin and 1.3αCaMKII

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. RP51A-No5'ss-NoBS-NoPseudoBS

    Plasmid

    #100987

    Purpose

    RP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BS

    Depositor

    Aaron Hoskins

    Article

    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

    Insert

    RP51A (RPS17A Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pU6-TREX1 g3 Cas9-T2A-mCherry

    Plasmid

    #164252

    Purpose

    Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

    Depositor

    John Maciejowski

    Article

    Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

    Insert

    sgTREX1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSTAR2

    Plasmid

    #166940

    Purpose

    STAR-based multi-level gene expression controller (MLC)

    Depositor

    Thomas Gorochowski

    Article

    Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

    Insert

    Circuit expressing GFP- Small transcription activating RNAs
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PTAC

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSTAR

    Plasmid

    #166939

    Purpose

    STAR-based multi-level gene expression controller (MLC)

    Depositor

    Thomas Gorochowski

    Article

    Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

    Insert

    Genetic circuit expressing GFP- Small Transcription activating RNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PTAC

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVG97

    Plasmid

    #164849

    Purpose

    Plasmid for replacement of PEST sequence in strains with pr-yEVenus-PEST-ADH1t construct

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    Part of URA3 5' UTR and yEVenus with stop codon for recombination with pr-yEVenus-PEST-ADH1t:URA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS314_U6_A34G_A35G

    Plasmid

    #81232

    Purpose

    U6 telestem mutant (A34G/A35G) for in vivo studies

    Depositor

    Aaron Hoskins

    Article

    Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

    Insert

    U6 RNA with A34G/A35G mutation (snR6 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    U6 telestem mutant (A34G/A35G)

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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