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pHTC-HaloTag_ACTB:1-375

Plasmid

#210971

Purpose

Mammalian expression of full-length human ACTB. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

ACTB:M1-F375 (ACTB Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTC-HaloTag_DCAF12:1-453

Plasmid

#210967

Purpose

Mammalian expression of full-length human DCAF12. C-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF12:M1-S453 (DCAF12 Human)

Tags

HaloTag

Expression

Mammalian

Mutation

R131Q natural variant (VAR_035322)

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pHTN-HaloTag_ACTB:1-375

Plasmid

#210970

Purpose

Mammalian expression of full-length human ACTB. N-terminal HaloTag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

ACTB:M1-F375 (ACTB Human)

Tags

HaloTag

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pNLF1-C_DCAF1:1038-1400

Plasmid

#210963

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_CORO1A:1-461

Plasmid

#210964

Purpose

Mammalian expression of full-length human CORO1A. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CORO1A:M1-K461 (CORO1A Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_CORO1A:1-461

Plasmid

#210965

Purpose

Mammalian expression of full-length human CORO1A. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CORO1A:M1-K461 (CORO1A Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_DCAF1:1038-1400

Plasmid

#210962

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_FBXW11:1-542

Plasmid

#210952

Purpose

Mammalian expression of full-length human FBXW11. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXW11:M1-R542 (FBXW11 Human)

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_SKIC8:1-305

Plasmid

#211038

Purpose

Mammalian expression of full-length human SKIC8. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

SKIC8:M1-I305 (SKIC8 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_COPB2:1-597

Plasmid

#211055

Purpose

Mammalian expression of human COPB2 fragment M1 to A597. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

COPB2:M1-A597 (COPB2 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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RLC12

Pooled Library

#163954

Purpose

Targets open reading frames and non-coding RNAs in M. tuberculosis.

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Expression

Bacterial

Use

CRISPR

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only
RLC11

Pooled Library

#163955

Purpose

Targets open reading frames and non-coding RNAs in M. smegmatis.

Depositor

Jeremy Rock

Article

Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

Expression

Bacterial

Use

CRISPR

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only
pGP-AAV-syn-jGCaMP8s-WPRE

Plasmid

#162374

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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H3K9me3 biosensor

Plasmid

#120802

Purpose

FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Truncated YPet-HP1-EV linker-ECFP-mouse histone H3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLU-CMV-Flag-hATG5 WT

Plasmid

#111803

Purpose

Mammalian Expression of hATG5

Depositor

Jun Hee Lee

Article

Kim et al Elife. 2016 Jan 26;5. pii: e12245. doi:

Insert

hATG5 (ATG5 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-GFP

Plasmid

#222996

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-EGFP

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pIG-641 JE-TRAC-BCMA(BB)28z-P2A

Plasmid

#186127

Purpose

BCMA-CAR DNA production

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

BCMA-CAR (TNFRSF17 Human)

Use

CRISPR

Mutation

WT

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-BSD

Plasmid

#224122

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-BSD

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-jGCaMP8m-WPRE

Plasmid

#162375

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL_SRSF2_WT_mCherry

Plasmid

#84020

Purpose

expression of SRSF2 in mammalian cells

Depositor

Robert Bradley

Article

Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

Insert

SRSF2 (SRSF2 Human)

Use

Lentiviral

Tags

2TA-mCherry and Flag

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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