We narrowed to 31,818 results for: LIS;

1. pET-28a RUVBL2 Delete

   Plasmid

   #159143

   Purpose

   Expression and protien purification of a mutated RUVBL2

   Depositor

   Eric Erquan Zhang

   Article

   Ju et al Sci Transl Med. 2020 May 6;12(542). pii:

   Insert

   RUVBL2 (RUVBL2 Human)
   

   Expression

   Bacterial

   Mutation

   amino acid 2-456, Thr127-Glu233 mutated to GPPG

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-SARS2-S-G614 (C-flag)

   Plasmid

   #156421

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

   Depositor

   Hyeryun Choe, Michael Farzan

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS CoV2)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV pTRE-FLEX-EGFP-WPRE-bGHpA

   Plasmid

   #65449

   Purpose

   Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   EGFP
   

   Use

   AAV

   Promoter

   tetO

   Available Since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNK7187

   Plasmid

   #219761

   Purpose

   MoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)

   Depositor

   Karen Sarkisyan

   Article

   Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

   Insert

   PpASCL
   

   Expression

   Plant

   Promoter

   p35s_0.4kb - 5'UTR TMV omega

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTwist-SARS-CoV-2 Spike-deltaC18 EG.5.1

   Plasmid

   #212454

   Purpose

   Encodes SARS-CoV-2 variant EG.5.1 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike EG.5.1 (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Jan. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA3-AUP1-FLAG-DATDCUE

   Plasmid

   #185340

   Purpose

   Assessing domain requirement sof AUP1

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Tags

   FLAG, 6xHis

   Expression

   Mammalian

   Mutation

   deletion of aa 90-337 corresponding to the acyl t…

   Available Since

   May 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TCF4-DN in pLX303

   Plasmid

   #42592

   Depositor

   William Hahn

   Article

   Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

   Insert

   TCF7L2 (TCF7L2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   31 first amino acids of hTcf4 have been deleted

   Promoter

   CMV

   Available Since

   Feb. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pUC19-Top2A-5X Gly-Venus-T2A-Hygro

   Plasmid

   #211115

   Purpose

   For tagging the C-term of Top2A with Venus

   Depositor

   Job Dekker

   Article

   Hildebrand et al Mol Cell. 2024 Mar 13:S1097-2765(24)0014

   Inserts

   5' C-term Top2A homology arm (TOP2A Human)
   Venus
   T2A
   Blastocidin Resistance
   5' C-term Top2A homology arm (TOP2A Human)
   

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   n/a

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. dCas9-p300 HAT

   Plasmid

   #179544

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) driven by AF4 promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of human p300 HAT domain (aa 1287-1664) (EP300 Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pXPR_023d-sgCiCh2-2

    Plasmid

    #202444

    Purpose

    Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2

    Depositor

    William Hahn

    Article

    Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

    Insert

    Chromosome 2 gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYLCRISPR/Cas9pUbi-N

    Plasmid

    #106331

    Purpose

    Expression of Cas9 in plants, ZmUbi promoter, Kanamycin selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    ZmUbi

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA6-N-3XFLAG-Ubxd8

    Plasmid

    #123599

    Depositor

    Bruce Beutler

    Article

    Choi et al Science. 2019 May 10;364(6440). pii: 364

    Insert

    Fas associated factor family member 2 (Faf2 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8m-p10

    Plasmid

    #162384

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Tags

    6xHis

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CL20_mEGFP_PAX5_NCOA5

    Plasmid

    #205885

    Purpose

    Express mEGFP-tagged fusion protein, PAX5_NCOA5 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    PAX5_NCOA5 (PAX5, NCOA5 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-Hygro-EF1A-BRCT2-Linker-eGFP

    Plasmid

    #176085

    Purpose

    EGFP fused to the C-terminus of a BRCT2 domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    BRCT2 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-Hygro-EF1A-BRCT1-Linker-eGFP

    Plasmid

    #176074

    Purpose

    EGFP fused to the C-terminus of a BRCT1 domain & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    BRCT1 (XRCC1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1A

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSUMO His6 SUMO SnRK2.6 (11-362)

    Plasmid

    #177847

    Purpose

    Bacterial Expression of SnRK2.6

    Depositor

    Karsten Melcher

    Article

    Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

    Insert

    SNF1-related protein kinase 2.6 (OST1 Mustard Weed)
    

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNK5712

    Plasmid

    #219752

    Purpose

    pGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pGAP - nnH3H_v2 - tAOX
    

    Expression

    Yeast

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pICOZ-SB100X[EF1α-CD19 4-1BB]

    Plasmid

    #218328

    Purpose

    Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

    Depositor

    Haoyi Wang

    Article

    Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

    Insert

    CD19 CAR mammalian expression cassette
    

    Expression

    Mammalian

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMCB306-GFP-mito

    Plasmid

    #229232

    Purpose

    Expresses GFP localized to mitochondria

    Depositor

    Suzanne Pfeffer

    Article

    Bagnoli et al Proc Natl Acad Sci U S A. 2025 Feb 4;122

    Insert

    EGFP-MAOA (MAOA Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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