We narrowed to 9,345 results for: yeast expression

1. pDEST-3xFLAG-Ubc7

   Plasmid

   #212801

   Purpose

   Constitutive expression of 3xFLAG-tagged budding yeast Ubc7 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   Ubc7
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT-TO-Ubc13

   Plasmid

   #212806

   Purpose

   Constitutive or doxycycline-inducible expression of budding yeast Ubc13 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   Ubc13
   

   Expression

   Mammalian

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-FRT-TO-Mms2

   Plasmid

   #212807

   Purpose

   Constitutive or doxycycline-inducible expression of budding yeast Mms2 in mammalian cells

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Insert

   Mms2
   

   Expression

   Mammalian

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPG-GPD-TRPC

   Plasmid

   #190113

   Purpose

   Expression vector for fungal (Aspergillus flavus) genes based on Aspergillus parasiticus pyrG gene selection

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Fungal Genet Biol. 2017 Jul;104:29-37. d

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSCL.108

   Plasmid

   #184977

   Purpose

   Express -Eco1 LYP1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 12
   

   Expression

   Yeast

   Mutation

   LYP1 donor E27stop

   Promoter

   Gal7

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSCL.106

   Plasmid

   #184975

   Purpose

   Express -Eco1 CAN1 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 12
   

   Expression

   Yeast

   Mutation

   CAN1 donor G444stop

   Promoter

   Gal7

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSCL.72

   Plasmid

   #184984

   Purpose

   Express Cas9/-Eco1 RT fusion, using linker1

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Cas9-fusion_linker1-Eco1RT
   

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCL.95

   Plasmid

   #184986

   Purpose

   Express Cas9/-Eco1 RT fusion, using linker2

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Cas9-fusion_linker2-Eco1RT
   

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSCL.94

   Plasmid

   #184985

   Purpose

   Express -Eco1 RT/Cas9 fusion, using linker2

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1RT-fusion_linker2-Cas9
   

   Tags

   SV40NLS

   Expression

   Yeast

   Mutation

   human codon optimized RT

   Promoter

   Gal1-10

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSCL.037

    Plasmid

    #184966

    Purpose

    Express -Eco1 dead RT and ncRNA in yeasst

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT
    

    Expression

    Yeast

    Mutation

    Human codon optimized RT, YXDD catalytic region m…

    Promoter

    Gal7

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSCL.71

    Plasmid

    #184983

    Purpose

    Express -Eco1 RT/Cas9 fusion, using linker1

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1RT-fusion_linker1-Cas9
    

    Tags

    SV40NLS

    Expression

    Yeast

    Mutation

    human codon optimized RT

    Promoter

    Gal1-10

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCL.112

    Plasmid

    #184981

    Purpose

    Express -Eco1 FAA1 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12
    

    Expression

    Yeast

    Mutation

    FAA1 donor P233stop

    Promoter

    Gal7

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSCL.002

    Plasmid

    #184972

    Purpose

    Express -Eco1 ADE2 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12
    

    Expression

    Yeast

    Mutation

    ADE2 donor P272stop

    Promoter

    Gal7

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSCL.110

    Plasmid

    #184979

    Purpose

    Express -Eco1 TRP2 editing ncRNA and gRNA

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12
    

    Expression

    Yeast

    Mutation

    TRP2 donor E64stop

    Promoter

    Gal7

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMM4_14L

    Plasmid

    #183063

    Purpose

    Plasmid expressing a biosensor for acetic acid and pHluorin: BM3R1-Haa1-mTurquoise2 under RET2p, mCherry under HREsYGP1p and sfpHluorin under TDH3p with flanks for genome integration into HO locus

    Depositor

    Yvonne Nygård

    Article

    Mormino et al Microb Cell Fact. 2022 Oct 15;21(1):214.

    Inserts

    mCherry
    sfpHluorin
    BM3R1-HAA1-mTurquoise2
    

    Use

    Synthetic Biology

    Tags

    mCherry and mTurquoise2

    Expression

    Yeast

    Promoter

    RET2, Synthetic promoter, and TDH3

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIPKAH

    Plasmid

    #185869

    Purpose

    Knocking out GPP1 and expressing a phosphoketolase pathway (Lactobacillus reuteri xfp and Methanosarcina barkeri pta)

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    GPP1(-220,80)> PAg.TEF1>hphMX6>TAg.TEF1-PENO2> Lr.xfp>T_TPI1-PTDH3>Mb.pta>TFBA1-GPP1(754, 1400)
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pML104-natR412

    Plasmid

    #162042

    Purpose

    Expresses Cas9 and contains natR412 guide which targets the natR gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

    Insert

    natR412 guide
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pML107-natR854

    Plasmid

    #162043

    Purpose

    Expresses Cas9 and contains natR854 guide which targets the natR gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

    Insert

    natR854 guide
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGST-pol1-N2-2A2

    Plasmid

    #160991

    Purpose

    Express a fragment of yeast pol1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    pol1
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F58A/D62A

    Available Since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGST-pol1-N1-2A2

    Plasmid

    #160990

    Purpose

    Express a fragment of yeast pol1 protein

    Depositor

    Zhiguo Zhang

    Article

    Li et al Sci Adv. 2020 Aug 26;6(35):eabb5820. doi

    Insert

    pol1
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F58A/D62A

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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