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PB CRIV Gc Spike H6

Plasmid

#240163

Purpose

Plasmid for making PiggyBac stable cell line expressing Cristoli virus (CRIV) glycoprotein Gc spike with a C-terminal His6 tag

Depositor

Stephen Graham

Article

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

CRIV Gc spike

Use

Piggybac

Tags

6xHis

Expression

Mammalian

Mutation

contains residues 478-906 only

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLG-mLRRC45 (209 a.a.)

Plasmid

#240068

Purpose

ITT reporter plasmid for cold stress

Depositor

Hodaka Fujii

Article

Maruyama et al Biol Methods Protoc. 2025 Jun 14;10(1):b

Insert

LRRC45 C-terminal 209 a.a. (Lrrc45 Mouse)

Use

Retroviral

Tags

LexA DNA-binding (DB) domain + Gal4 transactivati…

Expression

Mammalian

Promoter

LTR

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pdCas9-tdTomato

Plasmid

#232631

Purpose

catalytically inactive dCas9 for localization; labeled with tdTomato

Depositor

Karsten Rippe

Insert

dCas9-tdTomato

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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CAG-VEGF120-T2A-mCherry

Plasmid

#229134

Purpose

Retrovirus driving overexpression of VEGF120 plus mCherry reporter

Depositor

Elizabeth Kirby

Article

Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

Inserts

VEGF120 (Vegfa Mouse)
mCherry

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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Tom20-EYFP-miLID

Plasmid

#210291

Purpose

mitochondria marker and "anchor" for optogenetics

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

Tom20-EYFP-miLID

Tags

EYFP and iLID

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pINQ-NbH4

Plasmid

#198690

Purpose

Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)

Depositor

Gualberto González Sapienza

Article

Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

Insert

anti-SARS-CoV-2 Nucleocapsid nanobody H4

Tags

6xHis tag, Avitag, OmpA signal peptide, and Ribos…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pOSV00157

Plasmid

#222937

Purpose

Kill switch plasmid for DNA sensor construction using Bacillus subtilis

Depositor

Ophelia Venturelli

Article

Cheng et al Nat Commun. 2023 Apr 10;14(1):2001. doi:

Insert

TxpA-RatA toxin-antitoxin

Expression

Bacterial

Promoter

Phyperspank

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL3346 PB-nicking guide-blast

Plasmid

#226665

Purpose

PiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidin

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

protective nicking sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-marmoset cGAS-HA

Plasmid

#186864

Purpose

Doxycycline-dependent expression of marmoset cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Marmoset cGAS with C-terminal HA tag

Use

Retroviral

Tags

HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PGK-LCB3_v1.2-NotchGal4VP64-T2A-mCherry (pZYW047)

Plasmid

#194230

Purpose

Lentiviral expression vector expressing LCB3 SynNotch and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Insert

PGK-LCB3-SynNotch-T2A-mCherry

Use

Lentiviral

Mutation

E45D mutation in LCB3

Promoter

PGK

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pPU-HSA-miR30-L1221

Plasmid

#174221

Purpose

All-in-one control rescue-control shRNA knockdown vector

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

HSA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPU-HSA-miR30-MDA5

Plasmid

#174222

Purpose

All-in-one control rescue-MDA5 shRNA knockdown vector

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

HSA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGFP-N1_CBPdCFID

Plasmid

#221909

Purpose

Transient expression of GFP-tagged CBPdeltaCFID in mammalian cells

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBPdeltaCFID (CREBBP Human)

Tags

GFP

Expression

Mammalian

Mutation

deletion of the CBP-FUS interaction domain (CFID)…

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-IFT56

Plasmid

#218727

Purpose

Expresses N-terminally EGFP-tagged IFT56 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nozaki et al J Cell Sci. 2016 Dec 7. pii: jcs.197004.

Insert

IFT56 (TTC26 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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AA435

Plasmid

#215962

Purpose

Fragmid fragment: (guide cassette) guide expression; positive control cell surface

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

U6_v1; DR_v0 [EnAs]; sgCD274_v1; DR_v1 [EnAs]; sgADGRE5_v3; DR_v2; sgCD4_v1; DR_v3; sgDPP4_v1

Use

CRISPR; Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPAP_scFv

Plasmid

#183733

Purpose

Expresses anti-lysozyme scFv antibody

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Anti-lysozyme scFv

Tags

His6-tag

Expression

Yeast

Promoter

AOX1 promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMBD2.1R (pc2813)

Plasmid

#211575

Purpose

Expresses MBD2 (aa1-152) RFP tagged in mammalian cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MBD2.1 aa1-152

Tags

RFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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β2AR-PPI-CALPon1.0 (APEX)

Plasmid

#213406

Purpose

Peroxidase (APEX) reporter for beta 2-adrenergic receptor (β2AR) and β-arrestin 2 interaction (component 1)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

β2AR-E5-TEVcs-APEX(201-250)-K5-EGFP-APEX(2-200)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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PET-HypaR-SpCas9-NLS-6xHis

Plasmid

#207387

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

HypaR-SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

R661A, N692A, M694A, Q695A, H698A

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-1 (a)

Plasmid

#204428

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

R627A K663A R683A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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