We narrowed to 32,761 results for: eng

1. pDestTol2-QUAS:GCaMP7a

   Plasmid

   #184817

   Purpose

   Used to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040).

   Depositor

   Marnie Halpern

   Article

   Spikol et al Elife. 2024 May 31;12:RP89516. doi: 10.7

   Insert

   QUAS:GCaMP7a-pA
   

   Use

   Zebrafish tol2 transgenesis

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0.423

   Plasmid

   #203947

   Purpose

   Level 0 part. 2,4-diacetylphloroglucinol (DAPG)-inducible phlF_PphlF promoter.

   Depositor

   Alistair McCormick

   Article

   Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

   Insert

   phlF-PphlF
   

   Use

   Synthetic Biology

   Available Since

   May 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cas9m4

   Plasmid

   #47316

   Purpose

   Cas9 D10A+D839A+H840A+N863A

   Depositor

   George Church

   Article

   Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

   Insert

   Cas9m4
   

   Use

   CRISPR

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR_TRE3GS-->sGFP-P2A-mCherry

   Plasmid

   #162232

   Purpose

   Lentiviral vector for the expression of TRE3GS-->sGFP-P2A-mCherry in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   EGFP and mCherry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_TRE3GS-->sGFP-P2A-BFP

   Plasmid

   #162234

   Purpose

   Lentiviral vector for the expression of TRE3GS-->sGFP-P2A-BFP in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2020 Oct 16;370(6514):327-331.

   Insert

   EGFP and BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCBFRE-luc

   Plasmid

   #26897

   Depositor

   Nicholas Gaiano

   Article

   Yu et al Cell Stem Cell. 2008 May 8. 2(5):461-71.

   Insert

   CBFRE
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   Dec. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

7. ePE2

   Plasmid

   #202029

   Purpose

   Prime Editing plasmid for tag insertion

   Depositor

   Pengcheng Wei

   Article

   Li et al Plant Commun. 2023 Mar 7:100572. doi: 10

   Insert

   Cas9，
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. HHM-iATPSnFR1.1

   Plasmid

   #102547

   Purpose

   expresses ATP sensor in bacteria

   Depositor

   Baljit Khakh

   Article

   Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

   Insert

   iATPSnFR1.1
   

   Expression

   Bacterial

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pP450cam

   Plasmid

   #85085

   Purpose

   Expresses P450cam (CYP101A1) in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   P450cam
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-myc-Wnk1

    Plasmid

    #38779

    Depositor

    Melanie Cobb

    Article

    Xu et al J Biol Chem. 2000 Jun 2;275(22):16795-80

    Insert

    Wnk1 (Wnk1 Rat)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

11. p2lox-cAMPr Y205A

    Plasmid

    #102438

    Purpose

    cAMPr with a point mutation Y205A which should render PKA-C catalytically dead

    Depositor

    Justin Blau

    Article

    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

    Insert

    cAMPr
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Mutation

    PKA-C Y205A

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRVV661

    Plasmid

    #108282

    Purpose

    Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

    Depositor

    Richard Mann

    Article

    Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

    Insert

    PhiC31 attP sites
    

    Use

    CRISPR

    Available Since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ132-tRNA

    Plasmid

    #179211

    Purpose

    Golden Gate entry vector; 2nd gRNA with tRNA

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Promoter

    no promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJW1311

    Plasmid

    #61254

    Purpose

    Template for cloning sgRNA(F+E) to generate new PU6::sgRNAs by PCR-fusion

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    sgRNA(F+E) targeting Y61A9L9.1
    

    Use

    CRISPR; Cloning template

    Available Since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMT-3P2A

    Plasmid

    #111772

    Purpose

    Quadcistronic construct: Co-express four genes by 3 P2A peptides

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Type

    Empty backbone

    Use

    Cloning intermediate

    Promoter

    T7

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEG03

    Plasmid

    #154138

    Purpose

    This plasmid carries fucO and aldA under the control of P_gyrA promoter, which is used for study about Improving ethylene glycol utilization in Escherichia coli fermentation.

    Depositor

    Kang Zhou

    Article

    Panda et al Biochemical Engineering Journal

    Insert

    fucO, aldA
    

    Expression

    Bacterial

    Promoter

    P_gyrA

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET24b-SUMO-BG2.4

    Plasmid

    #199438

    Purpose

    expresses BAmGreen2.4 in bacterial cells with a SUMO tag

    Depositor

    Andrew Woolley

    Article

    Jang et al Nat Methods. 2023 Feb 23. doi: 10.1038/s

    Insert

    BAMGreen2.4
    

    Tags

    his tag and sumo

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCM271rfp

    Plasmid

    #59774

    Purpose

    pCMrfp with TrfA mutation (271R to C)

    Depositor

    Nathan Hillson

    Article

    Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

    Insert

    RFP
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRVV669

    Plasmid

    #108283

    Purpose

    Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

    Depositor

    Richard Mann

    Article

    Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

    Insert

    PhiC31 attP sites
    

    Use

    CRISPR

    Available Since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAPInt-sfGFP-G418

    Plasmid

    #236481

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on G418.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    sfGFP
    

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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