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pKLV2-U6gRNA5(hRNF31_2b)-PGKpuro2ABFP-W

Plasmid

#208415

Purpose

Lentiviral gRNA plasmid targeting human RNF31 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RNF31 (RNF31 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hXIAP_1k)-PGKpuro2ABFP-W

Plasmid

#208420

Purpose

Lentiviral gRNA plasmid targeting human XIAP gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

XIAP (XIAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hXIAP_2b)-PGKpuro2ABFP-W

Plasmid

#208421

Purpose

Lentiviral gRNA plasmid targeting human XIAP gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

XIAP (XIAP Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRNF31_1k)-PGKpuro2ABFP-W

Plasmid

#208414

Purpose

Lentiviral gRNA plasmid targeting human RNF31 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RNF31 (RNF31 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Chim-Pchi-Q61A

Plasmid

#231520

Purpose

Expresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Pchi HMG Q61A flanked with N- and C-termini of mSox2

Use

Lentiviral

Mutation

Point mutation at position 61 Q to A of the HMG d…

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Chim-Salhel-I-K57E

Plasmid

#231519

Purpose

Expresses Salpingoeca helianthica chimeric Sox-I mutant in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Salhel-I HMG K57E flanked with N- and C-termini of mSox2

Use

Lentiviral

Mutation

Point mutation at position 57 K to E of the HMG

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-Chim-Pchi-Q61A/E64M

Plasmid

#231521

Purpose

Expresses Pigoraptor chileana chimeric Sox mutant in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Pchi HMG Q61A/E64M flanked with N- and C-termini of mSox2

Use

Lentiviral

Mutation

Point mutations at position 61 Q to A and 64 E to…

Available Since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pHIV-EF1a-Il1a-ZsGreen

Plasmid

#231987

Purpose

Express IL-1alpha-ZsGreen fusion protein

Depositor

Brian Ruffell

Article

Hanggi et al Cancer Cell. 2024 Nov 13:S1535-6108(24)0

Insert

IL-1alpha-ZsGreen fusion construct

Use

Lentiviral

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0_shRNAHsCavin1_Del4UC1_EGFP

Plasmid

#229691

Purpose

Lentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish mutant Cavin1 (Del4UC1)

Depositor

Alpha Yap

Article

Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

Inserts

shRNA for hCavin1 (CAVIN1 Human)
mutant cavin1b (Del4UC1) (cavin1b Zebrafish)

Use

Lentiviral

Tags

EGFP

Mutation

Del4UC1

Promoter

LTR viral promoter

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pLL5.0_shRNAHsCavin1_DrCavin1b_EGFP

Plasmid

#229690

Purpose

Lentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish Cavin1 (Cavin1 rescue)

Depositor

Alpha Yap

Article

Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.

Inserts

shRNA for hCavin1 (CAVIN1 Human)
cavin1b (cavin1b Zebrafish)

Use

Lentiviral

Tags

EGFP

Promoter

LTR viral promoter

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry

Plasmid

#229135

Purpose

Retrovirus driving mCherry reporter

Depositor

Elizabeth Kirby

Article

Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

Insert

mCherry

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4-CMV-3xFLAG-NLS/SV40-AcGFP-NES/PKI-P2A-Puro

Plasmid

#228399

Purpose

Encodes 3xFLAG-NLS/SV40-AcGFP-NES/PKI which is a AcGFP with an N-terminal SV40 NLS and a C-terminal PKI NES that shuttles between the nucleus and the cytoplasm

Depositor

Dirk Daelemans

Article

Vercruysse et al Commun Biol. 2024 Nov 23;7(1):1560. doi:

Insert

3xFLAG-NLS/SV40-AcGFP-NES/PKI

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pC130: pAAV.CMV-CasRx-VEGFA sgRNA

Plasmid

#203443

Purpose

Plasmid expressing active RfxCas13d with VEGFA mRNA targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA
RfxCas13d

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pM149: pAAV.CMV-Cas13e-VEGFA sgRNA

Plasmid

#203447

Purpose

Plasmid expressing active Cas13e with VEGFA mRNA targeting gRNA

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pM150: pAAV.CMV-Cas13e-VEGFA sgRNA array

Plasmid

#203448

Purpose

Plasmid expressing active Cas13e with an array of three VEGFA mRNA targeting gRNAs

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA array
Cas13e

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pTag4C_SP-DRD2-V2R-NTEV-tcs-GV-2xHA

Plasmid

#227103

Purpose

DRD2 receptor for split TEV assay

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

DRD2-NTEV (DRD2 Human)

Tags

2xHA

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pWT-IFl_WT-IFr

Plasmid

#192207

Purpose

Wildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and wildtype I-F Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pScr-IFl_WT-IFr

Plasmid

#192208

Purpose

Scrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Scrambled I-F Leader and wildtype I-F Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IFr

Plasmid

#192209

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-F Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IEr

Plasmid

#192210

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-E Locus DNA

Expression

Bacterial

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge