We narrowed to 180,175 results for: Mal;

1. pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)

   Plasmid

   #114092

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a (S542R/K548V/N552R)
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   S542R, K548V and N552R

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-NLS(SV40)x2-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-gs-UGI-NLS(SV40)(AsBE1.4) (RTW1008)

   Plasmid

   #114080

   Purpose

   CAG promoter expression plasmid for human codon optimized AsBE1.4

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.4)
   

   Tags

   2x SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-hAsCas12a(E174R/S542R)-NLS(nucleoplasmin)-3xHA (AAS826)

   Plasmid

   #114091

   Purpose

   CAG promoter expression plasmid for human codon optimized AsCas12a(E174R/S542R) nuclease with C-terminal NLS and HA tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized AsCas12a (E174R/S542R)
   

   Tags

   NLS(nucleoplasmin) and 3xHA

   Expression

   Mammalian

   Mutation

   E174R and S542R

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-rAPOBEC1-gs-XTEN-gs-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-UGI-NLS(SV40)(AsBE1.1) (RTW1351)

   Plasmid

   #114079

   Purpose

   CAG promoter expression plasmid for human codon optimized AsBE1.1

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   human codon optimized DNase-inactive (D908A) enAsCas12a fused to N-terminal rAPOBEC1 and C-terminal UGI (AsBE1.1)
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. Mirror GFP[ENE(C8351G)-mascRNA(mut 8356-8370)](pAVA3871)

   Plasmid

   #239352

   Purpose

   Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of MALAT1 ENE(C8351G)-mascRNA(mut 8356-8370) reporter.

   Depositor

   Andrei Alexandrov

   Article

   Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

   Insert

   MALAT1 ENE(C8351G)-mascRNA(mut 8356-8370)
   

   Expression

   Mammalian

   Mutation

   MALAT1 ENE(C8351G)-mascRNA(mut 8356-8370: ctacgac…

   Available Since

   Aug. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. CRaf-IRES-pErk reporter

   Plasmid

   #226170

   Purpose

   Used to transiently express Myc-tagged CRaf with the pErk reporter. Used in LABEL-seq MAPK activity assays.

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Insert

   Myc-CRaf-IRES-3xFlag-tdMCP-Erk2 (MAPK1, RAF1 Human)
   

   Tags

   3xFlag-tdMCP and Myc

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX304-BCL-XL-499/500R

   Plasmid

   #203575

   Purpose

   Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499, TRCN0000033500) in mammalian cells.

   Depositor

   Abhishek Chakraborty

   Article

   Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   V5-tagged

   Expression

   Mammalian

   Mutation

   silent point mutations to make resistant shRNA 49…

   Promoter

   CMV

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Akna-mOrange2

   Plasmid

   #196880

   Purpose

   Neuron-specific expression of the centrosomal protein Akna fused to mOrange2

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akna-mOrange2 (Akna Mouse)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST-Myc-FBW7 Delta WD40 (-2 to 7)

   Plasmid

   #197454

   Purpose

   The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 propellars 2 to 7 are deleted. Used for mammalian overexpression and detection by western blotting.

   Depositor

   Omar Khan

   Article

   Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

   Insert

   FBW7 Delta-WD40 propeller (FBXW7 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   FBW& dimerization domain deleted.

   Promoter

   CMV

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDEST-Myc-FBW7 WD40 (All deleted)

    Plasmid

    #197455

    Purpose

    The plasmid expresses delta WD40 deleted version of Myc-tagged FBW7 human isoform 1. WD40 All are deleted. Used for mammalian overexpression and detection by western blotting.

    Depositor

    Omar Khan

    Article

    Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

    Insert

    FBW7 (FBXW7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    FBW& dimerization domain deleted.

    Promoter

    CMV

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.3-hAP1 1-419

    Plasmid

    #183644

    Purpose

    To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    ATP6AP1 (ATP6AP1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    truncated ATP6AP1 mutant; contains only amino aci…

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-sPDGFRalpha-Fc-Y206S

    Plasmid

    #136439

    Purpose

    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (Y206S mutant)

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRalpha (PDGFRA Human)
    

    Tags

    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…

    Expression

    Mammalian

    Mutation

    Extracellular domain of PDGFR-alpha (a.a. 1-524) …

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-sPDGFRalpha-Fc-V242K

    Plasmid

    #136440

    Purpose

    Mammalian expression plasmid for soluble ectodomain of PDGFR-alpha fused to IgG1 Fc (V242K mutant)

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    PDGFRalpha (PDGFRA Human)
    

    Tags

    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and L…

    Expression

    Mammalian

    Mutation

    Extracellular domain of PDGFR-alpha (a.a. 1-524) …

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. SP-dCas9-VPR

    Plasmid

    #63798

    Purpose

    SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    SP-dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. (attB) tdMCP-tagged BRaf with MS2-circRNA

    Plasmid

    #226163

    Purpose

    Used to integrate 3xFlag-tdMCP-BRaf and MS2-circRNA barcodes into RNA-Protein Landing Pad cell lines

    Depositor

    Dustin Maly

    Article

    Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

    Inserts

    3xFlag-tdMCP-BRaf (BRAF Human)
    IRES-mCherry-P2A-PuroR
    MS2-circRNA barcode
    

    Use

    Bxb1 attb, no promoter

    Tags

    3xFlag-tdMCP

    Expression

    Mammalian

    Available Since

    Aug. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. EF1a_HNF4A_P2A_Hygro_Barcode

    Plasmid

    #120450

    Purpose

    Barcoded lentiviral vector to express HNF4A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    HNF4A (HNF4A Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)

    Plasmid

    #182287

    Purpose

    Encodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    

    Tags

    nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    Y306A/Y384F (AF) double mutant of Methanosarcina …

    Promoter

    CMV and U6

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBABE-hygro-2xMyc-Rac1-WT

    Plasmid

    #128580

    Purpose

    For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

    Depositor

    Julian Downward

    Article

    Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

    Insert

    Rac1 (Rac1 Mouse)
    

    Use

    Retroviral

    Tags

    2xMyc epitope

    Expression

    Mammalian

    Available Since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB-CAGGS-dCas9-KRAB-MeCP2

    Plasmid

    #110824

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB-MeCP2
    

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PEmax_AAVS1_knockin_HDR_donor

    Plasmid

    #223000

    Purpose

    HDR donor plasmid for PEmax knockin at AAVS1 locus. Left_homology_arm-splicing_acceptor-T2A-NeoR-bGHpA-pEF1α-PEmax-IRES2-EGFP-WPRE-βglobinpA-right_homology_arm

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PEmax
    

    Use

    Hdr donor

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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