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p3E-SGTAP no-pA

Plasmid

#82599

Purpose

3' entry vector containing streptavidin binding protein (SBP) followed by a TEV cleavage site and 2 protein G copies without pA for tandem affinity purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

SBP-TEV-2x protein G

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3b2-FL3

Plasmid

#78388

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xFLAG
hygML

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3c-PK3

Plasmid

#78368

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xPK
natMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pMM763

Plasmid

#68900

Purpose

IPTG-inducible CRISPRi vector targeting PcfiA (PR1), constiutive NanoLuc, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

dCas9
LacI
sgRNA
NanoLuc

Mutation

G1104E

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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LI B-C mCherry

Plasmid

#54426

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

mCherry + 16 aa GS linker

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI B-C GFP

Plasmid

#54422

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

sGFP + 16 aa GS linker

Use

Cloning vector

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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hSyn1-DIO-mCherry-CAAX

Plasmid

#223673

Purpose

Cre-dependently expresses mCherry-CAAX in neurons

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mCherry-CAAX

Use

AAV and Cre/Lox

Promoter

hSyn1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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HisMS2_VLP_RSV

Plasmid

#175958

Purpose

Generation of MS2 Virus-Like Particles packaged with the sequence for the RSV P and M genes [GenBank: M74568.1]

Depositor

Paul Freemont

Article

Unruh et al medRxiv 2024.04.17.24305965

Inserts

P gene
M gene

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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lentiGuide_puro-P2A-mNeonGreen

Plasmid

#221464

Purpose

Lentiviral expression of an sgRNA with mNeonGreen fluorescent marker

Depositor

James Eshleman

Insert

mNeonGreen

Use

CRISPR and Lentiviral

Promoter

hU6, EF-1a

Available Since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAbaMCi-GFP-gfp_pJMP2768

Plasmid

#208889

Purpose

Acinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).

Depositor

Jason Peters

Article

Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

Insert

dCas9, sgRNA expression cassette, sfGFP

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ4004

Plasmid

#204401

Purpose

A 3OHC14-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

Inserts

mCinR
GFP

Use

Synthetic Biology

Promoter

PftsH and Synthetic promoter PCin0102D

Available Since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHS-439

Plasmid

#195471

Purpose

Lentiviral Integratable Inducible superIL-2 cytokine using synZiFTR ZF3

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF3 BS - ybTATA - super IL-2 - 2A -EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEY26

Plasmid

#191031

Purpose

cho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

cho-1(3.3-2.6k) (cho-1 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiGuide_GFP_NT135

Plasmid

#183118

Purpose

pLentiGuide modified to express GFP and a non-targeting sgRNA

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

NT135

Use

Lentiviral

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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EZ-L439

Plasmid

#169146

Purpose

Encodes OptoINVRT7 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2020 Dec 18;9(12):3254-3

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pJRH056

Plasmid

#171637

Purpose

Plasmid containing a U6 promoter expressing a spyCas9 tdTom sgRNA298 and CAG promoter expressing mTagBFP2

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-tdTomato
mTagBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CAG and U6

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pPM2212

Plasmid

#167552

Purpose

Encodes rfb region of Shigella flexneri serotype 2a, which expresses Shigella O antigen in E. coli K-12

Depositor

Renato Morona

Article

Macpherson et al Mol Microbiol. 1991 Jun;5(6):1491-9. doi

Insert

rfb region of Shigella flexneri serotype 2a

Use

Cosmid

Available Since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAG552-MAP4-redFAST

Plasmid

#160367

Purpose

Expresses MAP4-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

MAP4-redFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAG469-LifeAct-redFAST

Plasmid

#160365

Purpose

Expresses LifeAct-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

LifeAct-RedFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2-LA-nanoLuc-RA

Plasmid

#128692

Purpose

Donor plasmid that promotes integration of the NanoLuc coding sequence at the apobb.1 locus

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

apobb.1 NanoLuc

Use

Multisite-gateway assembly

Tags

NanoLuc

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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