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pEGFP-C3-PLK4 K41M-3xFLAG

Plasmid

#69838

Purpose

This plasmid encodes kinase dead PLK4 isoform 1 (K41M mutation) with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Publication

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

K41M

Promoter

CMV

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDEST47-ARF6-GFP

Plasmid

#67394

Purpose

Mammalian expression of hArf6 with C-term GFP tag

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL3-GFP

Plasmid

#67397

Purpose

Mammalian expression of hArl3 with C-term GFP tag

Depositor

Richard Kahn

Publication

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Cav3-YFP

Plasmid

#69507

Purpose

Expression of fluorescently tagged Caveolin-3 in mammalian cells

Depositor

Rob Parton

Publication

Pol et al Mol Biol Cell. 2004 Jan;15(1):99-110. Ep

Insert

Cav3 (Cav3 Mouse)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
pmPB2-VSV

Plasmid

#68038

Purpose

Expresses mouse Plexin-B2 with N-terminal VSV tag in mammalian cells

Depositor

Roland Friedel

Publication

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Plexin-B2 (Plxnb2 Mouse)

Tags

VSV

Expression

Mammalian

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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attB-GFP-Poly(A)‐NeoR

Plasmid

#65526

Purpose

NeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

Depositor

Sebastian Bultmann

Publication

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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attB-GFP

Plasmid

#65521

Purpose

Plasmid for the generation of GFP-knockin fusions via Bxb1-mediated recombination into MIN-tagged cell lines

Depositor

Sebastian Bultmann

Publication

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

attB-GFP

Use

Mouse Targeting; Bxb1

Expression

Mammalian

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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Slc17a7-IRES2-Cre targeting vector

Plasmid

#61574

Purpose

Target the Cre recombinase gene to the stop codon of the mouse Slc17a7 (VGlut1) gene

Depositor

Hongkui Zeng

Publication

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Slc17a7-IRES2-Cre

Use

Cre/Lox and Mouse Targeting

Available since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pFPL-Rg

Plasmid

#61654

Purpose

Gateway vector for generating red fluorescent protein fusions in fungi

Depositor

Mark Farman

Publication

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

monomeric red fluorescent protein

Expression

Plant

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOPINK-OTUD4 (OTU, aa 22-196)

Plasmid

#61413

Purpose

Expresses human OTUD4 (OTU domain) in E. coli.

Depositor

David Komander

Publication

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD4 (OTU domain-containing protein 4) (OTUD4 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-21 and aa 197-1114.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pTargeT gE

Plasmid

#60845

Purpose

Expression of the varicella zoster glycoprotein (gE) in mammalian cells

Depositor

Charles Grose

Publication

Carpenter et al J Virol. 2011 Sep;85(18):9414-24. doi: 1

Insert

ORF68 (ORF68 )

Expression

Mammalian

Promoter

CMV

Available since

Nov. 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pScalps_puro_mIL-2Rβ

Plasmid

#59916

Purpose

Expresses mouse interleukin-2 receptor Beta chain

Depositor

Silvia Monticelli

Publication

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

interleukin 2 receptor beta (Il2rb Mouse)

Use

Lentiviral

Available since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA SF-HERC2 F6

Plasmid

#55789

Purpose

Expresses N-terminal 3xFLAG/STREP tagged HERC2 (4421-4834aa)

Depositor

David Chan

Publication

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (4421-4834aa) (HERC2 Human)

Tags

3xFLAG and STREP

Expression

Mammalian

Promoter

CMV

Available since

July 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
NPC1 His6 EYFP

Plasmid

#53523

Depositor

Matthew Scott

Publication

Lopez et al J Neurosci. 2011 Mar 23;31(12):4367-78.

Insert

NPC1

Tags

EYFP and His6

Expression

Mammalian

Promoter

CMV

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

Plasmid

#51248

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Publication

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907,980,1006 mutated to A…

Promoter

EF1a

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only
pICH41414

Plasmid

#50337

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pBBRBB-Ppuf843-1200-DsRed

Plasmid

#50962

Purpose

Expressed DsRed using the Ppuf843-1200 promoter segment, for use in R. sphaeroides

Depositor

Claudia Schmidt-dannert

Publication

Tikh et al Appl Microbiol Biotechnol. 2014 Feb 9.

Insert

DsRed

Use

Synthetic Biology

Promoter

Ppuf843-1200

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-mRFP-ura4MX6

Plasmid

#49183

Purpose

genomic mRFP tagging in S. pombe

Depositor

Eishi Noguchi

Publication

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

mRFP

Expression

Yeast

Available since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-12Pk-kanMX6

Plasmid

#49176

Purpose

genomic 12Pk epitope tagging in S. pombe

Depositor

Eishi Noguchi

Publication

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

12Pk

Expression

Yeast

Available since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pT7-Venus-Emi1 (914)

Plasmid

#39854

Depositor

Jonathon Pines

Publication

Di Fiore et al J Cell Biol. 2007 May 7;177(3):425-37.

Insert

Emi1 (FBXO5 Human)

Use

In vitro transcription

Tags

Venus

Promoter

T7

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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