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pBABE-2xFLAG-2xSTREP-CCND1-Puro

Plasmid

#172634

Purpose

Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Publication

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

Plasmid

#169162

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

Depositor

John Diffley

Publication

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)

Tags

6His-3xFlag

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

Plasmid

#169163

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

Depositor

John Diffley

Publication

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-6His-nsp14 (ORF1ab SARS-CoV-2, Synthetic)

Tags

3xFlag-6His

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 T538N

Plasmid

#167181

Purpose

puromycin resistant lentiviral vector for expression of PAK5 T538N

Depositor

Christin Burd

Publication

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 T538N

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 V593I

Plasmid

#167179

Purpose

puromycin resistant lentiviral vector for expression of PAK5 V593I

Depositor

Christin Burd

Publication

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 V593I

Use

Lentiviral

Expression

Mammalian

Promoter

UBC

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-3NFLAG-mLIG3-179-936

Plasmid

#159316

Purpose

Mammalian cell expression of mouse LIG3-179-936

Depositor

Jianjun Zhao

Insert

mLIG3-179-936 (Lig3 Mouse)

Expression

Mammalian

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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p8195 MSCV-P C-FlagHA 6E7 R77S

Plasmid

#163309

Purpose

Expresses HPV6 E7 R77S

Depositor

Elizabeth White

Publication

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV6 E7 R77S

Use

Retroviral

Tags

FlagHA

Mutation

R77S

Promoter

MSCV LTR

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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p8194 MSCV-P C-FlagHA 31E7 R77S

Plasmid

#163308

Purpose

Expresses HPV31 E7 R77S

Depositor

Elizabeth White

Publication

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV31 E7 R77S

Use

Retroviral

Tags

FlagHA

Mutation

R77S

Promoter

MSCV LTR

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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p8193 MSCV-P C-FlagHA 18E7 R84S

Plasmid

#163307

Purpose

Expresses HPV18 E7 R84S

Depositor

Elizabeth White

Publication

Hatterschide et al J Virol. 2020 Aug 17;94(17). pii: JVI.01

Insert

HPV18 E7 R84S

Use

Retroviral

Tags

FlagHA

Mutation

R84S

Promoter

MSCV LTR

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS-Ala271 hygro

Plasmid

#158637

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

Depositor

Kevin Janes

Publication

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Mutation

Q271A

Available since

Nov. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Gln93,Ala271

Plasmid

#158632

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Publication

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway Cloning

Tags

3XFLAG

Mutation

E93Q/Q271A

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-MAVS-Ala148,Ala271

Plasmid

#158633

Purpose

Gateway entry vector for an inducible 3xFLAG-tagged MAVS mutant

Depositor

Kevin Janes

Publication

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Gateway Cloning

Tags

3XFLAG

Mutation

Q148A/Q271A

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS-Ala148 hygro

Plasmid

#158636

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

Depositor

Kevin Janes

Publication

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Mutation

Q148A

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-MAVS-Gln93 hygro

Plasmid

#158635

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS mutant construct

Depositor

Kevin Janes

Publication

Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

Insert

MAVS (MAVS Human)

Use

Lentiviral

Tags

3XFLAG

Mutation

E93Q

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-deltaKRMR-3xFLAG

Plasmid

#130676

Purpose

Expresses human SMCHD1 deltaKRMR with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Publication

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 deltaKRMR (SMCHD1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

K1987_R1990del

Promoter

CMV

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-NOS-IN133.3xFLAG-WPRE

Plasmid

#127869

Purpose

pAAV plasmid expressing an NOS-IN133.3xFLAG fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Publication

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1 (Nos1 Mouse)

Use

AAV

Tags

3xFLAG

Mutation

Amino acids 1-133 only

Promoter

hSyn

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1(+)/CUG-stop-nLuc-3XFLAG

Plasmid

#127309

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG-PEST

Plasmid

#127313

Purpose

Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from CUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and PEST

Expression

Mammalian

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GGG-stop-nLuc-3XFLAG

Plasmid

#127311

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from negative control GGG start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AAA-stop-nLuc-3XFLAG

Plasmid

#127310

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from negative control AAA start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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