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BtARR3 short (1-392)

Plasmid

#236272

Purpose

Bacterial expression of bovine arrestin-3 short (1-392) L393Stop

Depositor

Vsevolod Gurevich

Article

Vishnivetskiy et al Curr Protoc. 2023 Sep;3(9):e832. doi: 10

Insert

arrestin-3 (ARR3 Bovine)

Expression

Bacterial

Mutation

short, (1-392) L393Stop

Promoter

lac

Available Since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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qTAG-KO-Puro

Plasmid

#227262

Purpose

Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-PIP5K1B

Plasmid

#202722

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:PIP5K1B (PIP5K1B Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-KO-Blast

Plasmid

#227263

Purpose

Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSWEET-bgaB

Plasmid

#210272

Purpose

Xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.

Depositor

Eric Brown

Article

Bhavsar et al Appl Environ Microbiol. 2001 Jan;67(1):4

Insert

bgaB

Expression

Bacterial

Promoter

PxylA

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-HIS-FLAG-AtUBQ11

Plasmid

#222720

Purpose

Expression HIS-FLAG-AtUBQ11 in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtUBQ11 (UBQ11 Mustard Weed)

Tags

2xFLAG and 6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TagBFP2- PIP4K2BD278K

Plasmid

#202729

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:PIP4K2B (D278K) (PIP4K2B Human)

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pMM117

Plasmid

#127212

Purpose

BeYDV replicon with constitutive Luc expression, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

Luc, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pRC319

Plasmid

#61272

Purpose

Also referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.

Depositor

Timothy Lu

Article

Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

Insert

Cas9, tracrRNA, crRNA

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pPAiRFP1-N1

Plasmid

#44272

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Nat Commun. 2013 Jul 10;4:2153. doi: 10.

Insert

PAiRFP1

Expression

Mammalian

Mutation

G127D/S141R/M163L/Q168L/A203V/G218S/R220P/V244F/A…

Promoter

CMV

Available Since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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pNP561

Plasmid

#222723

Purpose

Target and Recombinase Golden Gate Landing Pad Backbone for LT-RT with T7 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Tags

6xHis

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pDD382

Plasmid

#91830

Purpose

Lox2272-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Lox2272-SEC-Lox2272

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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CFP-tTA-ER-C3

Plasmid

#134569

Purpose

Expresses a CFP-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion protein

Depositor

Susan Janicki

Article

Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

Insert

tTA-ER

Tags

CFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pG12-Rluc8

Plasmid

#170272

Purpose

Renilla reniformisluciferase (RLuc8) expression in mammlian cells. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

RLuc8

Expression

Mammalian

Promoter

12xUAS with TATA-box minimal promoter

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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Lamin A (R527P)-mRFP

Plasmid

#124272

Purpose

Lamin A mutant (R527P), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.

Depositor

Eric Schirmer

Insert

LMNA (R527P) (LMNA Human)

Tags

mRFP

Expression

Mammalian

Mutation

LMNA (R527P)

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pET22b_PfSSB

Plasmid

#103000

Purpose

The expressed protein is a Plasmodium falciparum single-stranded DNA-binding protein, PfSSB

Depositor

Martin Webb

Article

Chisty et al PLoS One. 2018 Feb 21;13(2):e0193272. do

Insert

PfSSB

Expression

Bacterial

Promoter

T7

Available Since

May 24, 2018

Availability

Academic Institutions and Nonprofits only

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pegRNA_LRRK2-3

Plasmid

#199272

Purpose

pegRNA used to induce LRRK2 (c. 6055 G > A) mutation

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LRRK2 6055GtoA pegRNA

Expression

Mammalian

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pSC-hTDG-PreE2

Plasmid

#52272

Purpose

Bacterial expression of human TDG C-terminally fused to SUMO-E2 and GST

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Insert

hTDG (TDG Human)

Tags

Ubc9-GST

Expression

Bacterial

Promoter

T7

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pMSSM59: pCMV-HA-NLS-SpuFz1-D300R

Plasmid

#205272

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-D300R

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Mutation

D300R

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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Cherry-tTA-ER-C3

Plasmid

#134567

Purpose

Expresses a Cherry-tTA (tetracycline transcriptional activator)-ER (estrogen receptor hormone binding domain) fusion protein

Depositor

Susan Janicki

Article

Rafalska-Metcalf et al PLoS One. 2010 Apr 21;5(4):e10272. doi:

Insert

tTA-ER

Tags

Cherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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