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shNTC

Plasmid

#73549

Purpose

Non-targeting control shRNA cloned in retroviral vector pMLP (MSCV-based vector expressing shRNA in a mir30 context).

Depositor

Gerardo Ferbeyre

Publication

Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

Insert

shNTC

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only
pLJM1-EGFP-BarcodeV3

Plasmid

#78631

Purpose

GESTALT V3 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Publication

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V3

Use

Lentiviral

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLJM1-EGFP-BarcodeV4

Plasmid

#78630

Purpose

GESTALT V4 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Publication

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V4

Use

Lentiviral

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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mPlum-CAF1-C-10

Plasmid

#55953

Purpose

Localization: Chromatin Assembly Factor 1, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Insert

CAF1 (Chaf1a Mouse)

Tags

mPlum

Expression

Mammalian

Promoter

CMV

Available since

March 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTSN7A

Plasmid

#131319

Purpose

Expressing synthetic TetR-GFP protein in N. crassa (from the his-3 locus)

Depositor

Eugene Gladyshev

Publication

Nguyen et al Fungal Genet Biol. 2020 Mar;136:103316.

Insert

TetR-GFP

Use

Unspecified

Tags

EGFP

Promoter

TrpC (from pCSN44)

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_GPR137B_WT_V5

Plasmid

#83017

Purpose

Gateway Donor vector containing GPR137B, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Publication

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

GPR137B (GPR137B Human)

Use

Gateway Cloning

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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TFORF3452

Plasmid

#144928

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Publication

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF460 (ZNF460 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_H3962D

Plasmid

#187131

Purpose

mammalian cell expression of 3xFLAG-HUWE1 H3962D

Depositor

Eric Fischer

Publication

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

H3962D

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEMS1080

Plasmid

#29016

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple208 (SLITRK6 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pEMS1079

Plasmid

#29015

Purpose

Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested

Depositor

Elizabeth Simpson

Publication

Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

Insert

Ple207 (SLITRK6 Human)

Use

Pleiades promoter project [sic, pleaides plieades]

Tags

EGFP-Cre-NLS

Available since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE43

Plasmid

#90381

Purpose

RUNX - Runx1 gene reporter

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

RUNX

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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nuc-yHS1

Plasmid

#159166

Purpose

Nuclear labile heme reporter

Depositor

Amit Reddi

Publication

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

nuc-yHS1

Tags

SV40 NLS

Expression

Yeast

Promoter

GPD

Available since

Sept. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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yHS1-M7A,H102A

Plasmid

#159152

Purpose

Cytosolic labile heme reporter

Depositor

Amit Reddi

Publication

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Insert

yHS1-M7A,H102A

Expression

Yeast

Mutation

Mutated both Met 7 and His 102 of the cyt b562 mo…

Promoter

GPD

Available since

Sept. 21, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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LV-Let7d-5p

Plasmid

#117055

Purpose

Overexpression of Let-7d-5p using miR-451 backbone to enable DICER independent expression along with orange fluorescent protein (OFP) to track miRNA expression

Depositor

Michele De Palma

Publication

Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

Insert

Let-7d-5p - OFP

Use

Lentiviral

Promoter

SFFV promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hSDH5

Plasmid

#113046

Purpose

Expression of WT hSDH5

Depositor

Edward A. Berry

Publication

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

hSDH5 (SDHAF2 Human)

Expression

Bacterial

Mutation

Insert begins at amino acid 39

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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H6hSDHA

Plasmid

#113045

Purpose

Hetero expression of His-tagged human SDHA

Depositor

Edward A. Berry

Publication

Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

Insert

H6hSDHA (SDHA Human)

Tags

His6 (MGSSHHHHHHSQDP-)

Expression

Bacterial

Mutation

Insert begins at amino acid 43

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-Crispr-V2-HMID.v1

Plasmid

#103062

Purpose

LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes

Depositor

Jay Shendure

Publication

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

Cas9 plus expressed GESTALT V1-V5 guide

Use

Lentiviral

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pTX209

Plasmid

#89269

Purpose

Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA01 and OsPDS-gRNA02

Depositor

Daniel Voytas

Publication

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Insert

OsPDS-gRNA01 and OsPDS-gRNA02

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Promoter

Maize Ubiquitin1 promoter

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hP14Gal4

Plasmid

#84364

Purpose

Gal4-PRDM14 funsion plasmid for luciferase recruitment assay

Depositor

Danny Reinberg

Publication

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

PRDM14 (PRDM14 Human)

Tags

Gal4 and HA tag

Expression

Mammalian

Mutation

silent mutations at coding silent 1068CtoT, 1629T…

Promoter

CMV/TO

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(rhesus)

Plasmid

#79033

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(rhesus)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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