We narrowed to 2,446 results for: PRK

Showing: 861 - 880 of 2446 results

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pRK5-mEGFP-HMGB1-MUT-RorKtoA

Plasmid

#194558

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R or K > A" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

R or K > A substitutions after Lys185

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-RtoA

Plasmid

#194557

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein, "R > A" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

R > A substitutions after Lys185

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG wildtype, catalytic active lipin 1

Plasmid

#32005

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 (Lpin1 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-MUT-Full-length

Plasmid

#194550

Purpose

Mammalian Expression of mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

K184Rfs*44

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK172-6xHis-0N4R-P301S-tau-HiBiT

Plasmid

#187835

Purpose

Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

6XHis-0N4R-P301S-tau-HiBiT

Tags

6xHistidine and HiBiT

Expression

Bacterial

Promoter

T7

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-TGF beta Type II receptor Flag

Plasmid

#31719

Depositor

Rik Derynck

Insert

TGF beta Type II Receptor (TGFBR2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only
pRK5 TGF beta type I receptor Flag

Plasmid

#14831

Depositor

Rik Derynck

Article

Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

Insert

TGF beta type I receptor (Tgfbr1 Rat)

Tags

flag

Expression

Mammalian

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG 17xS/T->A, catalytic active lipin 1

Plasmid

#32007

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 (Lpin1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

S106A, S150A, (S281A, T282A), S285A, S287A, S293A…

Promoter

CMV, SP6

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only
pRK5 FLAG wildtype, catalytic dead lipin 1

Plasmid

#32006

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

Lipin1 (Lpin1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

D712E and D714E

Promoter

CMV, SP6

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pRK DPC4 Flag (aka: Smad4 Flag)

Plasmid

#12627

Depositor

Rik Derynck

Article

Zhang et al Nature. 1996 Sep 12. 383(6596):168-72.

Insert

DPC4 (SMAD4 Human)

Tags

flag

Expression

Mammalian

Available Since

Oct. 10, 2006

Availability

Academic Institutions and Nonprofits only
pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)

Plasmid

#99144

Purpose

Inducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139A

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

UPF1 (UPF1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

First 127 codons are optimized to reduce GC conte…

Promoter

pTight

Available Since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG 17xS/T->A, catalytic dead lipin 1

Plasmid

#32008

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

Lipin1 (Lpin1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

D712E, D714E AND S106A, S150A, (S281A, T282A), S2…

Promoter

CMV, SP6

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only
prk5-MFSD12-TEV-3xFLAG-3xHA PM mutant

Plasmid

#149507

Purpose

Transient expression of human MFSD12 with recoded nucleotide sequence. Dileucine motif LL256-257 has been mutated to AA.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

MFSD12 (MFSD12 Human)

Use

Transient expression

Tags

TEV-3XHA-3XFLAG

Expression

Mammalian

Mutation

Codon Optimized Sequence, p.LL256-257AA

Promoter

CMV

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG membrane-bound lipin-1 (TMD)

Plasmid

#120277

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Tags

FLAG and in-frame fusion with the TMD Golgi-local…

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-TGF beta type I receptor (mL45)

Plasmid

#34701

Depositor

Ying Zhang

Article

Yu et al EMBO J. 2002 Jul 15;21(14):3749-59.

Insert

TGF beta type I receptor (Tgfbr1 Rat)

Tags

Flag

Expression

Mammalian

Mutation

N265A, D267A, N268A

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 494-1216 (USP)

Plasmid

#78588

Purpose

Expression flag-USP19 494-1216 (USP) in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 494-1216 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

deleted amino acid 1-493 and mutated 1216 residue…

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pRK5-TGF beta type I receptor (mL45TD)

Plasmid

#34702

Depositor

Ying Zhang

Article

Yu et al EMBO J. 2002 Jul 15;21(14):3749-59.

Insert

TGF beta type I receptor (Tgfbr1 Rat)

Tags

Flag

Expression

Mammalian

Mutation

T202D, N265A, D267A, N268A

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pRK5 Flag RagC(GD)/RagB(CTD) chimera

Plasmid

#112774

Purpose

express Flag-RagC (G domain) fused with with RagB (C terminal domain)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (G domain) fused with RagB (C terminal domain) (RRAGB Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagB(GD)/RagC(CTD) chimera

Plasmid

#112773

Purpose

express Flag-RagB (Gdomain) fused with RagC (C terminal domain)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagB (G domain) fused with RagC (C terminal domain) (RRAGC Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG

Plasmid

#238286

Purpose

For overexpression of mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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